COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-03114-0001-0000-2
Chemical Name: o-Chlorobenzalmalononitrile (CS)
CASRN: 2698-41-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 171099_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Test Failure 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 02/08/1983    Trial Call: Test Failure   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Acetone
0         
50 1047 557 0.532 11.14 25.5 0.000
Test Chemical o-Chlorobenzalmalononitrile (CS)
3.3       
50 1045 709 0.678 14.18 33.0 27.533
10         
0 0 0 0.000 0.00 33.0 N/A
Positive Control Mitomycin-C
 0.01      
5 101 173 1.713 34.60 25.5 221.971
Trend: 4.306        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 03/07/1983    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Acetone
0         
50 1042 494 0.474 9.88 25.7 0.000
Test Chemical o-Chlorobenzalmalononitrile (CS)
6         
50 1046 640 0.612 12.80 32.0 29.059
7         
50 1048 730 0.697 14.60 32.0 46.927
8         
50 1036 757 0.731 15.14 32.0 54.126
9         
0 0 0 0.000 0.00 32.0 N/A
Positive Control Mitomycin-C
 0.001     
50 1041 604 0.580 12.08 25.7 22.385
Mitomycin-C
 0.01      
5 105 165 1.571 33.00 25.7 231.463
Trend: 7.780        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/08/1983    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Acetone
0         
50 1044 464 0.444 9.28 25.5 0.000
Test Chemical o-Chlorobenzalmalononitrile (CS)
1         
50 1035 494 0.477 9.88 25.5 7.391
3.3       
50 1040 531 0.511 10.62 25.5 14.880
10         
50 1044 799 0.765 15.98 33.0 72.198
33         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
2         
5 105 161 1.533 32.20 25.5 245.000
Trend: 9.629        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/07/1983    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Acetone
0         
50 1045 449 0.430 8.98 25.7 0.000
Test Chemical o-Chlorobenzalmalononitrile (CS)
10         
50 1042 605 0.581 12.10 32.0 35.132
12.5       
50 1040 729 0.701 14.58 32.0 63.141
15         
50 1043 730 0.700 14.60 32.0 62.895
17.5       
0 0 0 0.000 0.00 32.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1035 634 0.613 12.68 25.7 42.567
Cyclophosphamide
2         
5 104 139 1.337 27.80 25.7 211.065
Trend: 8.706        
Probability: 0.000