Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-03114-0009-0000-0
Chemical Name: o-Chlorobenzalmalononitrile (CS)
CASRN: 2698-41-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 668414
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 46r 76 58.5 42 27
    60 96 48 27
    68 104 56 28
Test Chemical o-Chlorobenzalmalononitrile (CS) 
 0.3125    
 74 90 62 28 27
    72 106 54 25
  
 0.625     
 168r 231 52 10
    68 94 174 85
  
1.25      
 74 65 71 32 38
    69 58 92 44
  
2.5       
 65 32 153 78 75*
    70 31 150 71
  
5         
   
Positive Control Ethyl Methane Sulfonate 
250         
 65 75 264 136 145*
    57 68 261 154

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 103 117 58 74
    89 133 186 70
    59# 92 192 108
    66 72 118 60
Test Chemical o-Chlorobenzalmalononitrile (CS) 
 0.3125    
 76 104 182 80 79
    69 87 160 78
  
 0.625     
 64 57 164 85 83
    85 112 206 80
  
1.25      
 74# 71 180 81 81
    72# 71 177 82
  
2.5       
 46 14 291 213 204*
    48 21 283 195
  
5         
   
Positive Control Ethyl Methane Sulfonate 
250         
 58 68 323 187 239*
    55 72 478 291

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination