Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03114-0009-0000-0
Chemical Name:	o-Chlorobenzalmalononitrile (CS)
CASRN:	2698-41-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	668414
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	46r	76	58.5	42	27
			60	96	48	27
			68	104	56	28
Test Chemical	o-Chlorobenzalmalononitrile (CS)	0.3125	74	90	62	28	27
			72	106	54	25	27
		0.625	168r	231	52	10
			68	94	174	85
		1.25	74	65	71	32	38
			69	58	92	44	38
		2.5	65	32	153	78	75*
			70	31	150	71	75*
		5

Positive Control	Ethyl Methane Sulfonate	250	65	75	264	136	145*
Positive Control			57	68	261	154	145*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	103	117	58	74
			89	133	186	70
			59#	92	192	108
			66	72	118	60
Test Chemical	o-Chlorobenzalmalononitrile (CS)	0.3125	76	104	182	80	79
			69	87	160	78	79
		0.625	64	57	164	85	83
			85	112	206	80	83
		1.25	74#	71	180	81	81
			72#	71	177	82	81
		2.5	46	14	291	213	204*
			48	21	283	195	204*
		5

Positive Control	Ethyl Methane Sulfonate	250	58	68	323	187	239*
Positive Control			55	72	478	291	239*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination