Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03129-0011-0000-9
Chemical Name:	o-Nitroanisole
CASRN:	91-23-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	578918
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	115	103	198	58	69
			100#	94	225	75
			139r	121	230	55
			99	103	219	74
Test Chemical	o-Nitroanisole	0.0125	94	114	158	56	49
			116	127	136	39
			111	101	171	52
		0.025	114	124	135	39	47
			101	99	173	57
			79	83	106	45
		0.05	99#	117	158	53	54
			92	96	171	62
			105	102	145	46
		0.1	114	116	179	52
			129r	105	149	38
			159r	125	201	42
		0.2	88#	73	195	74
			138r	81	216	52
			132r	83	217	55
		0.3	108	62	261	81	81
			92	42	219	79
			94	59	229	81
		0.5


Positive Control	Ethyl Methane Sulfonate	250	103	70	1101	356	362*
			73	50	920	420
			88	70	819	311

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	82	105	90	36	38
			68	108	64	32
			72	109	77	36
			61	78	90	49
Test Chemical	o-Nitroanisole	0.025	74	99	52	23	27
			72	85	45	21
			58	80	62	36
		0.05	78	76	86	37	28
			66	78	45	23
			74	88	57	26
		0.1	65	70	80	41	45
			66	56	80	41
			56	57	91	54
		0.15	89	45	191	71	63*
			54#	50	92	57
			72	43	133	62
		0.2	71#	42	141	67	69*
			77	40	183	80
			69	43	126	61
		0.3	67	13	297	149	152*
			49	10	181	123
			63	10	346	184
		0.4


Positive Control	Ethyl Methane Sulfonate	250	73	76	669	305	338*
			70	58	783	373
			84	68	847	336

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	106	107	123	39	36
			114	121	104	30
			88	80	95	36
			79	92	94	40
Test Chemical	o-Nitroanisole	0.025	65	72	58	30	34
			49	59	56	38	34
		0.05	79	75	105	44	37
			59	65	77	43
			62	62	46	25
		0.1	69	68	70	34	45
			60	47	75	42
			68	42	124	61
		0.15	70	62	86	41	44
			94	50	140	50
			84	52	103	41
		0.2	67	24	188	94	72*
			77	41	139	60
			56	29	105	63
		0.3	82	29	196	80	85*
			61	34	146	79
			68	24	193	95
		0.4


Positive Control	Ethyl Methane Sulfonate	250	56	43	616	370	424*
			73#	62	865	395
			57	37	857	506

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	105#	118	151	48	49
			140r	122	157	37
			101	105	147	49
			91#	77	136	50
Test Chemical	o-Nitroanisole	0.0078	72	78	155	71	64
			85	65	166	65
			102	64	167	55
		0.0156	83	81	176	71	81*
			71#	83	164	77
			56	50	158	94
		0.0313	86	48	204	79	84*
			117	102	193	55
			62	22	216	117
		0.0625	92	73	193	70	69
			74	73	146	66
			88	38	189	72
		0.125	111	49	168	51	56
			103#	54	182	59
			98	47	168	57
Positive Control	3-Methylcholanthrene	2.5	128r	67	609	159	213*
			104	55	668	213
			130r	57	632	162

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination