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Study Information
Study Attribute Value
CEBS Accession Number: 002-03131-0001-0000-1
Chemical Name: o-Nitrobenzoic acid
CASRN: 552-16-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 778268_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Test Failure 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/03/1985    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1024 549 0.536 10.98 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
167         
 50 1013 485 0.479 9.70 26.0 -10.698 
500         
 50 1009 547 0.542 10.94 26.0 1.117 
1670         
 50 1006 643 0.639 12.86 32.0 19.218 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1028 562 0.547 11.24 26.0 1.970
Mitomycin-C 
 0.01      
 5 102 257 2.520 51.40 26.0 369.960
Trend: 3.597        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/23/1985    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1033 507 0.491 10.14 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
750         
 50 1022 518 0.507 10.36 26.0 3.269 
970         
 50 1019 542 0.532 10.84 26.0 8.372 
1650         
 50 1036 546 0.527 10.92 26.0 7.380 
3000         
 0 0 0 0.000 0.00 36.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1035 522 0.504 10.44 26.0 2.760
Mitomycin-C 
 0.01      
 5 105 181 1.724 36.20 26.0 251.222
Trend: 1.264        
Probability: 0.103        

Trial #: 3    Activation: No Activation    Date: 08/13/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1028 510 0.496 10.20 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
750         
 50 1024 473 0.462 9.46 26.0 -6.893 
1500         
 50 1008 607 0.602 12.14 31.0 21.381 
2225         
 50 1008 601 0.596 12.02 31.0 20.181 
3000         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1034 1024 0.990 20.48 26.0 99.619
Mitomycin-C 
 0.01      
 5 101 185 1.832 37.00 26.0 269.210
Trend: 4.545        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/03/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1024 497 0.485 9.94 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
500         
 50 1018 457 0.449 9.14 26.0 -7.506 
1670         
 50 1019 468 0.459 9.36 26.0 -5.373 
5000         
 50 1014 591 0.583 11.82 33.0 20.086
Positive Control Cyclophosphamide 
 0.4       
 50 1020 778 0.763 15.56 26.0 57.153
Cyclophosphamide 
2         
 5 104 209 2.010 41.80 26.0 314.054
Trend: 3.015        
Probability: 0.001        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 04/23/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1015 426 0.420 8.52 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
1500         
 50 1025 503 0.491 10.06 26.0 16.923 
3000         
 50 1009 518 0.513 10.36 26.0 22.319 
5000         
 50 1003 507 0.505 10.14 26.0 20.438
Positive Control Cyclophosphamide 
 0.4       
 50 1032 573 0.555 11.46 26.0 32.291
Cyclophosphamide 
2         
 5 105 166 1.581 33.20 26.0 276.682
Trend: 2.916        
Probability: 0.002        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 08/13/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1009 420 0.416 8.40 26.0 0.000
Test Chemical o-Nitrobenzoic acid 
3000         
 50 1033 456 0.441 9.12 26.0 6.049 
4000         
 50 1009 486 0.482 9.72 26.0 15.714 
5000         
 50 1014 457 0.451 9.14 26.0 8.273
Positive Control Cyclophosphamide 
 0.4       
 50 1029 650 0.632 13.00 26.0 51.754
Cyclophosphamide 
2         
 5 101 167 1.653 33.40 26.0 297.225
Trend: 1.593        
Probability: 0.056