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Study Information
Study Attribute Value
CEBS Accession Number: 002-03136-0001-0000-6
Chemical Name: o-Phenanthroline
CASRN: 66-71-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 864285_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Weakly Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Test Failure 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/12/1983    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 495 0.474 9.90 26.3 0.000
Test Chemical o-Phenanthroline 
 0.2       
 50 1040 461 0.443 9.22 26.3 -6.510
Positive Control Mitomycin-C 
 0.001     
 50 1047 607 0.580 12.14 26.3 22.275
Mitomycin-C 
 0.01      
 5 105 200 1.905 40.00 26.3 301.732
Trend: 0.000        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/26/1983    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 554 0.528 11.08 25.8 0.000
Test Chemical o-Phenanthroline 
 0.2       
 25 525 258 0.491 10.32 25.8 -6.859 
 0.3       
 50 1035 905 0.874 18.10 35.5 65.725 
 0.4       
 0 0 0 0.000 0.00 35.5 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1046 786 0.751 15.72 25.8 42.420
Mitomycin-C 
 0.01      
 5 107 220 2.056 44.00 25.8 289.689
Trend: 9.924        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 08/02/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 476 0.456 9.52 25.5 0.000
Test Chemical o-Phenanthroline 
 0.2       
 50 1035 590 0.570 11.80 33.8 25.027 
 0.225     
 50 1030 619 0.601 12.38 33.8 31.810 
 0.25      
 50 1043 650 0.623 13.00 33.8 36.686 
 0.275     
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1053 705 0.670 14.10 25.5 46.843
Mitomycin-C 
 0.01      
 5 100 181 1.810 36.20 25.5 296.983
Trend: 5.117        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 06/15/1983    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 515 0.490 10.30 25.8 0.000
Test Chemical o-Phenanthroline 
16.7       
 50 1050 649 0.618 12.98 25.8 26.019 
50         
 0 0 0 0.000 0.00 34.8 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1050 849 0.809 16.98 25.8 64.854
Cyclophosphamide 
2         
 5 102 249 2.441 49.80 25.8 397.716
Trend: 3.928        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/12/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 480 0.459 9.60 26.3 0.000
Test Chemical o-Phenanthroline 
35         
 50 1040 642 0.617 12.84 26.3 34.393 
40         
 50 1044 762 0.730 15.24 33.3 58.902 
50         
 50 1046 685 0.655 13.70 33.3 42.572 
60         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1046 744 0.711 14.88 26.3 54.852
Cyclophosphamide 
2         
 5 104 235 2.260 47.00 26.3 391.937
Trend: 6.045        
Probability: 0.000