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Study Information
Study Attribute Value
CEBS Accession Number: 002-03138-0002-0000-9
Chemical Name: o-Phenylenediamine
CASRN: 95-54-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 773292_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 03/12/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: o-Phenylenediamine 
15.1       
 100 23 0.230 14.0 8 0.080 5.0 15 0.150 12.0 0 0.000 0.0 
20.1       
 50 17 0.340 32.0 2 0.040 4.0 15 0.300 30.0 0 0.000 0.0 
30         
 25 24 0.960 36.0 4 0.160 16.0 10 0.400 28.0 10 0.400 4.0
Positive Control: Mitomycin-C 
 0.05      
 50 20 0.400 24.0 10 0.200 16.0 10 0.200 14.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 24 0.960 52.0 10 0.400 32.0 14 0.560 36.0 0 0.000 0.0
Trend: 5.606 2.887 5.205  
Probability: 0.000 0.002 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/19/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: o-Phenylenediamine 
2513         
 200 77 0.385 13.0 6 0.030 2.5 21 0.105 9.5 50 0.250 2.5 
3750         
 200 30 0.150 6.0 1 0.005 0.5 9 0.045 4.5 20 0.100 1.0 
5000         
 200 71 0.355 9.5 4 0.020 1.0 17 0.085 6.5 50 0.250 2.5
Positive Control: Cyclophosphamide 
6.25      
 200 31 0.155 12.5 10 0.050 4.5 21 0.105 10.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 13 0.520 36.0 6 0.240 16.0 7 0.280 28.0 0 0.000 0.0
Trend: 1.560 -0.628 1.318  
Probability: 0.059 0.735 0.094  


Trial #:2   Activation: No Activation    Date: 02/19/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.0 0 0.000 0.0 3 0.015 1.0 0 0.000 0.0
Test Chemical: o-Phenylenediamine 
7.5       
 200 45 0.225 9.0 6 0.030 3.0 19 0.095 6.0 20 0.100 1.0 
11.3       
 200 75 0.375 19.0 13 0.065 4.5 42 0.210 16.0 20 0.100 1.0 
15         
 100 52 0.520 23.0 5 0.050 5.0 27 0.270 16.0 20 0.200 2.0
Positive Control: Mitomycin-C 
 0.05      
 200 62 0.310 22.0 31 0.155 14.0 31 0.155 11.5 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 12 0.480 28.0 3 0.120 12.0 9 0.360 24.0 0 0.000 0.0
Trend: 6.806 2.947 5.836  
Probability: 0.000 0.002 0.000