Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03139-0009-0000-7
Chemical Name:	o-Phenylphenol
CASRN:	90-43-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	904368
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	88	107	167	64	48
			116#	126	108	31
			86	87	122	47
			89	80	128	48
Test Chemical	o-Phenylphenol	2.5	67	69	139	69	49
			99	146	87	29	49
		5	77	99	101	44	55
			69	73	136	66	55
		10	93#	111	166.5	60	58
			69#	68	117	57	58
		20	86	72	102	40	42
			87#	84	117	45	42
		40	91	80	139	51
		80

Positive Control	Ethyl Methane Sulfonate	250	67	69	139	69	112*
Positive Control			80#	82	373.5	156	112*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	57	109	95	56	45
			54	91	64	40
			77	97	76	33
			57	103	91	53
Test Chemical	o-Phenylphenol	20	86	80	127	49	43
			73	70	86	39
			66	69	82	42
		30	94	77	115	41	40
			118	78	145	41
			97	76	108	37
		40	108	61	214	66	57
			107	53	178	56
			84	56	121	48
		50	95	39	195	68	85*
			79	34	201	84
			81	26	247	102
		60	103	16	397	128
Positive Control	Ethyl Methane Sulfonate	250	92	83	759	275	299*
Positive Control			78	74	751	323	299*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	57	88	130	76	59
			63#	106	116	61
			57	95	93	54
			64	111	88	46
Test Chemical	o-Phenylphenol	10	78	124	104	45	47
			59	106	86	49	47
		20	84	104	102	41	45
			66	132	99	50	45
		40	54	62	65	40	44
			71	47	102	48	44
		50	46	33	100	72	55
			44	35	50	38	55
		60	68	19	150	74	80
			44	9	115	87	80
		70

Positive Control	Methyl Methane Sulfonate	15	36	30	187	172	202*
Positive Control			22	20	153	232	202*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	90	116	49	52
			82	109	106	43
			103	102	197	64
			81	99	131	54
Test Chemical	o-Phenylphenol	10	93	104	157	56	61
			77	97	151	66	61
		20	68	71	120	59	64
			79	63	163	69	64
		40	60	15	277	154	130*
			71	12	225	105	130*
		50	68	7	318	155	146*
			66	8	273	137	146*
		60

Positive Control	Methyl Methane Sulfonate	15	30	20	222	245	206*
Positive Control			32	22	157	166	206*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	69	79	122	59	59
			76	105	129	57
			80	118	165	69
			68	97	107	52
Test Chemical	o-Phenylphenol	0.625	64	88	246	129	142*
			56	60	260	155	142*
		1.25	59	54	331	186	189*
			58	57	333	192	189*
		2.5	39	38	443	382	366*
			42	42	439	350	366*
		5	35	17	710	679	669*
			40	19	796	658	669*
Positive Control	3-Methylcholanthrene	2.5	30	16	663	737	691*
Positive Control			39	24	755	645	691*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	101	176	90	86
			70	88	172	82
			65	110	141	72
			76	101	225	99
Test Chemical	o-Phenylphenol	0.3125	73	106	112	51	65
			71	82	167	79	65
		0.625	60	85	86	48	51
			53	90	87	55	51
		1.25	60	72	105	58	65
			73	77	156	71	65
		2.5	46	25	149	108	97
			63	19	166	87	97
		5	29	4	192	222	219*
			38	4	247	216	219*
		10

Positive Control	3-Methylcholanthrene	2.5	47	34	645	454	583*
Positive Control			27	26	577	712	583*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	89	154	65	64
			104	99	210	68
			89	98	154	58
			76	114	147	64
Test Chemical	o-Phenylphenol	10	77	54	168	73	78
			68	51	171	83	78
		20	51	25	167	108	114*
			60	23	216	120	114*
		40	50	8	215	143	171*
			40	5	238	198	171*
		50	42	5	245	196	204*
			41	5	263	213	204*
		60
		70

Positive Control	3-Methylcholanthrene	2.5	58	31	449	256	268*
Positive Control			44	29	369	280	268*

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	102	121	50	53
			99	85	173	58
			89	115	129	48
			100	98	171	57
Test Chemical	o-Phenylphenol	7	65	64	112	58	56
			72	56	119	55	56
		14	53	20	148	92	99*
			45	17	141	106	99*
		21	24	7	95	132	128*
			35	8	129	125	128*
		28

Positive Control	3-Methylcholanthrene	2.5	57	33	433	255	266*
Positive Control			57	35	470	277	266*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination