Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-03142-0006-0000-8
Chemical Name: o-Toluidine
CASRN: 95-53-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 923898
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 96 88 116 40 37
    91 99 100 37
    110 113 110 33
    122r 131 114 31
Test Chemical o-Toluidine 
 0.0156    
 91 83 113 41 42
    83 76 102 41
    93 88 122 44
  
 0.0313    
 100 72 118 39 42
    87 90 113 43
    82 60 105 43
  
 0.0625    
 80 62 120 50 52
    61 51 112 62
    101 53 138 45
  
 0.125     
 101 33 173 57 65*
    72 21 165 76
    69 30 129 63
  
 0.25      
 90 20 179 66 59*
    90 22 138 51
    81 17 147 61
  
 0.5       
 105*
    72 10 225 104
    63 7 199 105
  
1         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 74 33 843 379 345*
    92# 47 988 359
    94 56 835 297

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 67 90 62 31 32
    67# 91 80 40
    64 102 59 31
    80 117 60 25
Test Chemical o-Toluidine 
 0.1       
 58# 41 71 40 40
    55# 40 66 40
    54# 35 61 38
  
 0.2       
 56 24 71 43 39
    56 23 61 36
    72 36 83 39
  
 0.3       
 56 18 90 54 52*
    69 26 96 46
    39 13 64 55
  
 0.4       
 87 19 107 41 54*
    58 11 107 61
    66 16 117 59
  
 0.5       
 44 14 110 83 71*
    72 9 171 79
    65 11 98 50
  
 0.6       
 84# 15 128 51 51
    72# 11 109 51
Positive Control Methyl Methane Sulfonate 
10         
 20 6 380 644 702*
    24 10 551 760
Methyl Methane Sulfonate 
250         

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination