Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03142-0006-0000-8
Chemical Name:	o-Toluidine
CASRN:	95-53-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	923898
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	96	88	116	40	37
			91	99	100	37
			110	113	110	33
			122r	131	114	31
Test Chemical	o-Toluidine	0.0156	91	83	113	41	42
			83	76	102	41
			93	88	122	44
		0.0313	100	72	118	39	42
			87	90	113	43
			82	60	105	43
		0.0625	80	62	120	50	52
			61	51	112	62
			101	53	138	45
		0.125	101	33	173	57	65*
			72	21	165	76
			69	30	129	63
		0.25	90	20	179	66	59*
			90	22	138	51
			81	17	147	61
		0.5					105*
			72	10	225	104
			63	7	199	105
		1


Positive Control	Ethyl Methane Sulfonate	250	74	33	843	379	345*
			92#	47	988	359
			94	56	835	297

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	67	90	62	31	32
			67#	91	80	40
			64	102	59	31
			80	117	60	25
Test Chemical	o-Toluidine	0.1	58#	41	71	40	40
			55#	40	66	40
			54#	35	61	38
		0.2	56	24	71	43	39
			56	23	61	36
			72	36	83	39
		0.3	56	18	90	54	52*
			69	26	96	46
			39	13	64	55
		0.4	87	19	107	41	54*
			58	11	107	61
			66	16	117	59
		0.5	44	14	110	83	71*
			72	9	171	79
			65	11	98	50
		0.6	84#	15	128	51	51
			72#	11	109	51	51
Positive Control	Methyl Methane Sulfonate	10	20	6	380	644	702*
			24	10	551	760	702*
	Methyl Methane Sulfonate	250

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination