Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03147-0011-0000-9
Chemical Name:	p,p'-Dichlorodiphenyl dichloroethylene
CASRN:	72-55-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	202120
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	84	104	107	43	47
			84	96	111	44
			69	85	104	50
			81	115	119	49
Test Chemical	p,p'-Dichlorodiphenyl dichloroethylene	3.125	84	106	101	40	39
			71	73	80	37	39
		6.25	57	74	104	60	59
			86	108	149	58	59
		12.5	75	82	126	56	70
			50	67	127	85	70
		25	47	40	100	71	74*
			64	63	150	78	74*
		50	39	9	257	220	224*
			50	7	340	228	224*
		100

Positive Control	Methyl Methane Sulfonate	15	31	20	170	185	192*
Positive Control			26	19	155	199	192*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	99	170	55	56
			87	114	154	59
			84	90	117	46
			82	96	157	64
Test Chemical	p,p'-Dichlorodiphenyl dichloroethylene	25	74	59	132	60	64
			77	63	157	68	64
		30	87	42	254	97	90*
			107	47	265	83	90*
		35	80	27	285	119	120*
			89	24	324	121	120*
		40	69#	12	354	171
		45	39	5	460	391	496*
			37	5	664	601	496*
Positive Control	Methyl Methane Sulfonate	15	55	28	419	255	263*
Positive Control			41	37	333	271	263*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination