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Study Information
Study Attribute Value
CEBS Accession Number: 002-03161-0003-0000-6
Chemical Name: p-Chloroaniline
CASRN: 106-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 883820_SCE
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Test Failure 
No Activation  3  Weakly Positive 
No Activation  4  Test Failure 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 07/01/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 377 0.363 7.54 26.0 -12.144
Dimethyl Sulfoxide 
0         
 50 1046 432 0.413 8.64 26.0 0.000
Test Chemical p-Chloroaniline 
 0.5       
 50 1041 437 0.420 8.74 26.0 1.643 
1.6       
 50 1041 406 0.390 8.12 26.0 -5.567 
5         
 50 1037 405 0.391 8.10 26.0 -5.436 
16         
 50 1035 495 0.478 9.90 26.0 15.801 
50         
 50 1046 443 0.424 8.86 26.0 2.546 
160         
 50 1041 524 0.503 10.48 26.0 21.879 
500         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1047 1399 1.336 27.98 26.0 223.533
Mitomycin-C 
 0.01      
 50 1033 1964 1.901 39.28 26.0 360.351
Trend: 3.405        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 08/06/1982    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1034 443 0.428 8.86 26.0 0.000
Negative (Not Specified) 
0         
 50 1036 406 0.392 8.12 26.0 -8.529
Test Chemical p-Chloroaniline 
200         
 0 0 0 0.000 0.00 26.0 N/A 
300         
 0 0 0 0.000 0.00 26.0 N/A 
400         
 0 0 0 0.000 0.00 26.0 N/A 
500         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1043 1512 1.450 30.24 26.0 238.364
Mitomycin-C 
 0.01      
 50 1046 2235 2.137 44.70 26.0 398.727
Trend: 0.000        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 08/23/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1041 402 0.386 8.04 28.0 -6.639
p-Chloroaniline 
0         
 50 1042 431 0.414 8.62 28.0 0.000
Test Chemical p-Chloroaniline 
50         
 50 1043 453 0.434 9.06 28.0 5.004 
100         
 50 1050 521 0.496 10.42 28.0 19.961 
200         
 50 1042 550 0.528 11.00 28.0 27.610 
300         
 0 0 0 0.000 0.00 0.0 N/A 
400         
 0 0 0 0.000 0.00 0.0 N/A 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1034 1246 1.205 24.92 28.0 191.332
Mitomycin-C 
 0.01      
 50 1040 1912 1.838 38.24 28.0 344.473
Trend: 4.301        
Probability: 0.000        

Trial #: 4    Activation: No Activation    Date: 08/23/1982    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1041 402 0.386 8.04 28.0 -6.639
Dimethyl Sulfoxide 
0         
 50 1042 431 0.414 8.62 28.0 0.000
Test Chemical p-Chloroaniline 
50         
 50 1025 504 0.492 10.08 37.0 18.877 
100         
 50 1036 503 0.486 10.06 37.0 17.381 
200         
 50 1029 582 0.566 11.64 37.0 36.741 
300         
 50 1029 604 0.587 12.08 37.0 41.910 
400         
 0 0 0 0.000 0.00 0.0 N/A 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1034 1246 1.205 24.92 28.0 191.332
Mitomycin-C 
 0.01      
 50 1040 1912 1.838 38.24 28.0 344.473
Trend: 5.958        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/01/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 426 0.406 8.52 26.0 3.803
Dimethyl Sulfoxide 
0         
 50 1047 410 0.392 8.20 26.0 0.000
Test Chemical p-Chloroaniline 
1.6       
 50 1047 467 0.446 9.34 26.0 13.902 
5         
 50 1043 447 0.429 8.94 26.0 9.443 
16         
 50 1046 453 0.433 9.06 26.0 10.593 
50         
 50 1045 479 0.458 9.58 26.0 17.053 
160         
 50 1045 469 0.449 9.38 26.0 14.609 
500         
 50 1043 472 0.453 9.44 26.0 15.563 
1600         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1044 1593 1.526 31.86 26.0 289.653
Trend: 2.017        
Probability: 0.022        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/06/1982    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1034 434 0.420 8.68 26.0 0.000
Negative (Not Specified) 
0         
 50 1044 378 0.362 7.56 26.0 -13.737
Test Chemical p-Chloroaniline 
100         
 50 1033 492 0.476 9.84 26.0 13.474 
200         
 50 1029 521 0.506 10.42 26.0 20.629 
300         
 50 1038 476 0.459 9.52 26.0 9.255 
400         
 50 1033 465 0.450 9.30 26.0 7.247 
500         
 50 1031 482 0.468 9.64 26.0 11.383 
600         
 50 1036 480 0.463 9.60 26.0 10.386
Positive Control Cyclophosphamide 
1.5       
 50 1035 1918 1.853 38.36 26.0 341.508
Cyclophosphamide 
2         
 50 1038 2959 2.851 59.18 26.0 579.170
Trend: 0.707        
Probability: 0.240