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Study Information
Study Attribute Value
CEBS Accession Number: 002-03161-0004-0000-7
Chemical Name: p-Chloroaniline
CASRN: 106-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 883820_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Weakly Positive* 
Induced Rat Liver S9  3_S9  Negative 
No Activation  4  Weakly Positive 
Induced Rat Liver S9  4_S9  Negative 

Trial #:1   Activation: No Activation    Date: 07/01/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Chloroaniline 
30         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
300         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
600         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 26 0.260 24.0 8 0.080 8.0 11 0.110 11.0 7 0.070 7.0
Mitomycin-C 
 0.5       
 100 42 0.420 33.0 20 0.200 17.0 13 0.130 13.0 9 0.090 7.0
Trend: 1.835 0.956 1.635  
Probability: 0.033 0.169 0.051  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/01/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Chloroaniline 
1.6       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
16         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
800         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 125 1.250 59.0 40 0.400 29.0 22 0.220 21.0 63 0.630 31.0
Trend: 0.000 0.000 0.000  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 07/01/1988    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: p-Chloroaniline 
400         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0 
500         
 200 11 0.055 5.5 2 0.010 1.0 9 0.045 4.5 0 0.000 0.0 
600         
 200 14 0.070 6.5 3 0.015 1.5 11 0.055 5.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 30 0.150 14.0 8 0.040 4.0 22 0.110 10.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 25 0.500 38.0 5 0.100 8.0 20 0.400 34.0 0 0.000 0.0
Trend: 3.052 1.831 2.477  
Probability: 0.001 0.034 0.007  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 07/01/1988    Harvest Time: 18 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: p-Chloroaniline 
400         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
600         
 200 2 0.010 0.5 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
5         
 200 37 0.185 17.0 9 0.045 4.5 28 0.140 13.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 18 0.360 30.0 3 0.060 6.0 15 0.300 26.0 0 0.000 0.0
Trend: -1.375 1.306 -1.375  
Probability: 0.915 0.096 0.915  


Trial #:3   Activation: No Activation    Date: 07/01/1988    Harvest Time: 17 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: p-Chloroaniline 
400         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0 
500         
 200 6 0.030 3.0 1 0.005 0.5 5 0.025 2.5 0 0.000 0.0 
600         
 200 15 0.075 7.0 6 0.030 3.0 9 0.045 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 39 0.195 17.0 19 0.095 7.5 20 0.100 10.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 32 0.640 40.0 18 0.360 26.0 14 0.280 24.0 0 0.000 0.0
Trend: 3.851 2.484 2.912  
Probability: 0.000 0.006 0.002  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 08/05/1982    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Chloroaniline 
100         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
300         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
550         
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 1.0 
600         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 115 1.150 61.0 26 0.260 25.0 48 0.480 34.0 41 0.410 36.0
Trend: 1.647 0.950 0.950  
Probability: 0.050 0.171 0.171  


Trial #:4   Activation: No Activation    Date: 08/05/1982    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Chloroaniline 
100         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
300         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 4 0.040 4.0 1 0.010 1.0 2 0.020 2.0 1 0.010 1.0 
550         
 100 6 0.060 6.0 2 0.020 2.0 3 0.030 3.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.5       
 100 58 0.580 39.0 24 0.240 22.0 18 0.180 16.0 16 0.160 14.0
Trend: 3.280 1.767 2.310  
Probability: 0.001 0.039 0.010  


Trial #:4_S9   Activation: Induced Rat Liver S9    Date: 06/30/1988    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: p-Chloroaniline 
400         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
500         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
600         
 200 5 0.025 2.5 1 0.005 0.5 4 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 25 0.125 12.0 5 0.025 2.5 20 0.100 10.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 14 0.280 28.0 4 0.080 8.0 10 0.200 20.0 0 0.000 0.0
Trend: 0.447 1.306 0.121  
Probability: 0.327 0.096 0.452