Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03161-0015-0000-9
Chemical Name:	p-Chloroaniline
CASRN:	106-47-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	147947
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	86	86	122	47	48
			89	109	128	48
			88	79	167	64
			116#	126	108	31
Test Chemical	p-Chloroaniline	100	60	27	183	101	109*
			61	36	214	117	109*
		150	56	20	310	184	168*
			73	27	335	153	168*
		200	44	17	178	134	165*
			47	27	277	195	165*
		250	47	19	226	160	192*
			50	25	336	223	192*
		300	54#	33	255	159
Positive Control	Ethyl Methane Sulfonate	250	67	69	435	216	186*
Positive Control			80#	82	373.5	156	186*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	108	167	85	102
			65	102	229	118
			62	97	200	108
			53	94	154	96
Test Chemical	p-Chloroaniline	25	50	95	221	148	146
			62	96	270	144	146
		50	43	77	251	195	156
			64	97	224	117	156
		100	47	51	289	204	180*
			55	84	257	155	180*
		200	49	122	194	133	159*
			47	70	260	184	159*
		400	39	25	243	206	189*
			58	27	299	172	189*
Positive Control	Ethyl Methane Sulfonate	250	60	54	643	355	375*
Positive Control			44	61	518	394	375*

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	120	145	55	35
			78	92	73	31
			56	93	38	23
			75	95	67	30
Test Chemical	p-Chloroaniline	25	76	57	301	132	93*
			74	72	119	54	93*
		50	66	59	139	70	102*
			53	43	212	134	102*
		100	90	89	207	77	80*
			68	62	170	83	80*
		200	126r	90	257	68
			47	44	136	97
		400	92	34	299	109	101*
			60	46	168	93	101*
Positive Control	3-Methylcholanthrene	2.5	92	87	264	96	102*
Positive Control			91	105	291	107	102*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	56	116	39	23	28
			62	89	53	28
			51	95	49	32
Test Chemical	p-Chloroaniline	12.5	58	77	47	27	36
			58	82	80	46	36
		25	58#	79	94.5	54	51*
			38#	58	54	48	51*
		50	55	60	109	66	81*
			36	39	104	95	81*
		100	47	43	128	91	131*
			30	22	156	171	131*
		200	16	4	73	152
			7r	2	41	186
		400

Positive Control	3-Methylcholanthrene	2.5	47	59	182	130	108*
Positive Control			55#	75	141	86	108*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	101	124	48	53
			83	81	157	63
			108	113	151	47
			107	105	175	54
Test Chemical	p-Chloroaniline	25	71	74	99	47	56
			70	57	136	65	56
		50	73	70	99	45	58
			60	51	127	71	58
		100	51	59	126	82	77
			62	61	132	71	77
		200	82	68	147	60	75
			61	46	163	89	75
		400	91	36	209	76	83*
			76	29	207	90	83*
Positive Control	3-Methylcholanthrene	2.5	56	27	565	335	316*
Positive Control			52	31	463	297	316*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination