Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03161-0016-0000-0
Chemical Name:	p-Chloroaniline
CASRN:	106-47-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	845528
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	85#	134	60	24	22
			93	114	49	18
			61	74	51	28
			76	78	43	19
Test Chemical	p-Chloroaniline	12.5	54	71	81	50	39*
			82	80	69	28	39*
		25	53	58	79	49	37*
			72#	83	54	25	37*
		50	77	75	94	41	44*
			41#	47	57	47	44*
		100	61	47	87	47	67*
			38	40	99	86	67*
		200	63	50	93	49	48*
			38#	30	54	48	48*
Positive Control	Ethyl Methane Sulfonate	250	120#	110	390	108	108*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	54#	90	121.5	75	71
			60	96	146	82
			61	96	124	68
			66	118	117	59
Test Chemical	p-Chloroaniline	25	57	85	73	43	60
			72	86	164	76	60
		50	61	82	72	39	54
			62	74	128	68	54
		100	63#	82	33	18	43
			50	67	103	69	43
		200	62	77	83	44	31
			63	75	33	18	31
		400	49	39	103	70	42
			61	52	27	15	42
Positive Control	Ethyl Methane Sulfonate	250	52	62	357	231	160*
Positive Control			51	71	138	90	160*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	76	101	212	93	77
			80	96	185	77
			84	104	188	75
			71	98	137	64
Test Chemical	p-Chloroaniline	50	63	65	254	134	145*
			58	68	246	141
			41	54	196	160
		100	74	58	351	159	166*
			63	58	288	152
			64	53	361	188
		200	55	32	349	213	170*
			68	43	260	127
			74	44	381	171
		400	60	13	472	264	260*
			58	10	411	238
			57	14	473	278
		600


Positive Control	Ethyl Methane Sulfonate	250	74	100	579	260	297*
Positive Control			74	89	742	333	297*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	71	103	126	59	58
			66	99	122	62
			98	95	169	58
			97	103	159	54
Test Chemical	p-Chloroaniline	25	59	60	599	339	314*
			68	65	589	289	314*
		50	55	36	599	365	315*
			85	52	675	265	315*
		100	47	11	950	681	627*
			52	18	903	573	627*
		200

Positive Control	3-Methylcholanthrene	2.5	48	19	843	590	667*
Positive Control			42	21	926	744	667*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Methanol	0	53	76	148	93	79
			58	107	122	70
			69	122	151	73
			57	95	137	80
Test Chemical	p-Chloroaniline	9.375	45	55	195	144	133*
			59	64	216	121	133*
		18.75	41	47	229	187	165*
			47	51	202	144	165*
		37.5	61	34	560	304	318*
			39	26	384	332	318*
		75	31	7	470	505	427*
			32	8	335	349	427*
		150

Positive Control	3-Methylcholanthrene	2.5	34	30	424	416	442*
Positive Control			29	21	402	467	442*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination