Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-03161-0017-0000-1
Chemical Name: p-Chloroaniline
CASRN: 106-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G13922
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 107 130 48 44
    86 93 104 40
Test Chemical p-Chloroaniline 
105         
 91 83 103 38 41
    101 86 132 43
  
131         
 147r 125 99 22
    82 62 162 66
  
164         
 70 55 61 29
    133r 113 127 32
  
205         
 95 69 81 29 35
    99 72 124 42
  
256         
 84 43 137 55 49
    78 41 102 44
  
320         
 77 25 101 44 51
    80 31 138 58
  
400         
 79 19 101 43 49
    80 16 135 56
  
500         
 55 4 102 62 62
    55 4 100 61
Positive Control Ethyl Methane Sulfonate 
500         
 53 43 593 371 361*
    49 37 512 351

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 91 98 40 31
    87 97 82 32
    102 119 72 24
    87 93 72 28
Test Chemical p-Chloroaniline 
83.9       
 109 116 145 44 37
    95 97 87 31
  
105         
 75 77 72 32 33
    87 85 87 33
  
131         
 72 71 104 48 40
    95 89 93 33
  
164         
 67 58 128 64 45
    106 95 85 27
  
205         
 98 84 88 30 35
    90 74 108 40
  
400         
 73 31 154 70
  
500         
 71 5 152 71 64*
    90 8 152 56
Positive Control Ethyl Methane Sulfonate 
500         
 53 35 978 619 543*
    54 36 821 505
    58 37 878 505

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95 86 96 34 34
    94 95 74 26
    111 111 132 40
    107 107 115 36
Test Chemical p-Chloroaniline 
147         
 93 80 112 40 38
    96 68 112 39
    92 79 98 36
  
230         
 90 64 117 43 47
    82 57 111 45
    84 69 130 52
  
288         
 80 52 135 56 47
    74 39 86 39
    81 48 111 46
  
450         
 92 39 102 37 43
    91 38 116 43
    80 38 118 49
  
500         
 87 15 130 50 65*
    77 11 168 73
    86 13 188 73
Positive Control Ethyl Methane Sulfonate 
500         
 56 24 1355 802 658*
    62 26 1076 580
    63 33 1109 591

Nonactivation Trial: 4 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 56 104 153 92 79
    52 88 143 92
    40r 87 135 113
    51 108 81 53
Test Chemical p-Chloroaniline 
226         
 61 144 172 94 71
    65 126 94 48
  
266         
 62 107 123 66 57
    70 119 99 47
  
313         
 51 57 111 72 86
    53 45 157 99
  
368         
 51 34 189 123 111
    55 43 164 99
  
434         
 43 18 156 121 127*
    41 18 164 132
  
510         
  
600         
   
Positive Control Ethyl Methane Sulfonate 
500         
 35 53 751 712 700*
    37 46 735 668
    33 43 703 721

Nonactivation Trial: 5 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 106 149 69 45
    78 98 91 39
    77 96 77 33
    85 101 99 39
Test Chemical p-Chloroaniline 
310         
 69 92 162 78 67
    68 78 115 56
  
344         
 65 74 134 69 70
    98 104 208 71
  
383         
 77 76 102 44 61
    68 71 158 78
  
425         
 66 67 127 64 56
    78 69 109 47
  
473         
 85 74 161 64 66
    63 68 127 68
  
525         
 101 75 124 41 53
    80 68 155 64
Positive Control Ethyl Methane Sulfonate 
500         
 44 46 859 656 627*
    44 43 811 612
    54 52 986 614

Nonactivation Trial: 6 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98# 94 73.5 25 23
    93 97 74 27
    90 98 58 22
    102# 111 63 21
Test Chemical p-Chloroaniline 
218         
 113# 160 85.5 25 19
    102# 125 42 14
  
256         
 94 107 81 29 30
    81 99 76 31
  
302         
 82 97 56 23 24
    103 128 79 26
  
355         
 91 82 72 26 28
    97 90 88 30
  
491         
 78 52 76 32 42*
    81# 63 124.5 52
  
578         
 100 59 118 40
  
680         
 71 22 82 39
  
800         
   
Positive Control Ethyl Methane Sulfonate 
500         
 48# 37 850.5 597 538*
    48# 39 706.5 491
    52 36 825 525

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 105 107 42 35
    77 95 63 27
Test Chemical p-Chloroaniline 
15.7       
 70 32 161 76 79*
    65 29 159 82
  
19.7       
 72 28 189 88 84*
    75 30 178 79
  
24.6       
 130r 51 193 49
    80 29 160 67
  
30.7       
 70 20 210 101 86*
    66 20 143 72
  
38.4       
 65 13 134 69 69*
    84 20 177 70
  
48         
 85 15 144 57 72*
    57 9 149 87
  
60         
 59 8 189 106 114*
    56 7 202 121
Positive Control 3-Methylcholanthrene 
5         
 47 32 414 295 240*
    87 59 486 186

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 103 122 49 55
    89 100 137 52
    79 94 117 49
    89 104 184 69
Test Chemical p-Chloroaniline 
19.7       
 61 26 171 93 85
    74 34 169 76
  
24.6       
 67 25 181 90 92*
    60 20 171 95
  
30.7       
 65 17 100 51 68
    75 19 193 85
  
38.4       
 43 9 172 132 120*
    61 12 197 107
  
48         
 64 9 225 117 119*
    55 8 196 120
Positive Control 3-Methylcholanthrene 
5         
 43 28 459 354 242*
    66 44 372 188
    74 47 406 182

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination