Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03161-0017-0000-1
Chemical Name:	p-Chloroaniline
CASRN:	106-47-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G13922
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	107	130	48	44
Vehicle Control			86	93	104	40	44
Test Chemical	p-Chloroaniline	105	91	83	103	38	41
			101	86	132	43	41
		131	147r	125	99	22
			82	62	162	66
		164	70	55	61	29
			133r	113	127	32
		205	95	69	81	29	35
			99	72	124	42	35
		256	84	43	137	55	49
			78	41	102	44	49
		320	77	25	101	44	51
			80	31	138	58	51
		400	79	19	101	43	49
			80	16	135	56	49
		500	55	4	102	62	62
			55	4	100	61	62
Positive Control	Ethyl Methane Sulfonate	500	53	43	593	371	361*
Positive Control			49	37	512	351	361*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	91	98	40	31
			87	97	82	32
			102	119	72	24
			87	93	72	28
Test Chemical	p-Chloroaniline	83.9	109	116	145	44	37
			95	97	87	31	37
		105	75	77	72	32	33
			87	85	87	33	33
		131	72	71	104	48	40
			95	89	93	33	40
		164	67	58	128	64	45
			106	95	85	27	45
		205	98	84	88	30	35
			90	74	108	40	35
		400	73	31	154	70
		500	71	5	152	71	64*
			90	8	152	56	64*
Positive Control	Ethyl Methane Sulfonate	500	53	35	978	619	543*
			54	36	821	505
			58	37	878	505

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	86	96	34	34
			94	95	74	26
			111	111	132	40
			107	107	115	36
Test Chemical	p-Chloroaniline	147	93	80	112	40	38
			96	68	112	39
			92	79	98	36
		230	90	64	117	43	47
			82	57	111	45
			84	69	130	52
		288	80	52	135	56	47
			74	39	86	39
			81	48	111	46
		450	92	39	102	37	43
			91	38	116	43
			80	38	118	49
		500	87	15	130	50	65*
			77	11	168	73
			86	13	188	73
Positive Control	Ethyl Methane Sulfonate	500	56	24	1355	802	658*
			62	26	1076	580
			63	33	1109	591

Nonactivation Trial: 4 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	56	104	153	92	79
			52	88	143	92
			40r	87	135	113
			51	108	81	53
Test Chemical	p-Chloroaniline	226	61	144	172	94	71
			65	126	94	48	71
		266	62	107	123	66	57
			70	119	99	47	57
		313	51	57	111	72	86
			53	45	157	99	86
		368	51	34	189	123	111
			55	43	164	99	111
		434	43	18	156	121	127*
			41	18	164	132	127*
		510
		600

Positive Control	Ethyl Methane Sulfonate	500	35	53	751	712	700*
			37	46	735	668
			33	43	703	721

Nonactivation Trial: 5 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	106	149	69	45
			78	98	91	39
			77	96	77	33
			85	101	99	39
Test Chemical	p-Chloroaniline	310	69	92	162	78	67
			68	78	115	56	67
		344	65	74	134	69	70
			98	104	208	71	70
		383	77	76	102	44	61
			68	71	158	78	61
		425	66	67	127	64	56
			78	69	109	47	56
		473	85	74	161	64	66
			63	68	127	68	66
		525	101	75	124	41	53
			80	68	155	64	53
Positive Control	Ethyl Methane Sulfonate	500	44	46	859	656	627*
			44	43	811	612
			54	52	986	614

Nonactivation Trial: 6 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98#	94	73.5	25	23
			93	97	74	27
			90	98	58	22
			102#	111	63	21
Test Chemical	p-Chloroaniline	218	113#	160	85.5	25	19
			102#	125	42	14	19
		256	94	107	81	29	30
			81	99	76	31	30
		302	82	97	56	23	24
			103	128	79	26	24
		355	91	82	72	26	28
			97	90	88	30	28
		491	78	52	76	32	42*
			81#	63	124.5	52	42*
		578	100	59	118	40
		680	71	22	82	39
		800

Positive Control	Ethyl Methane Sulfonate	500	48#	37	850.5	597	538*
			48#	39	706.5	491
			52	36	825	525

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	105	107	42	35
Vehicle Control			77	95	63	27	35
Test Chemical	p-Chloroaniline	15.7	70	32	161	76	79*
			65	29	159	82	79*
		19.7	72	28	189	88	84*
			75	30	178	79	84*
		24.6	130r	51	193	49
			80	29	160	67
		30.7	70	20	210	101	86*
			66	20	143	72	86*
		38.4	65	13	134	69	69*
			84	20	177	70	69*
		48	85	15	144	57	72*
			57	9	149	87	72*
		60	59	8	189	106	114*
			56	7	202	121	114*
Positive Control	3-Methylcholanthrene	5	47	32	414	295	240*
Positive Control			87	59	486	186	240*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	103	122	49	55
			89	100	137	52
			79	94	117	49
			89	104	184	69
Test Chemical	p-Chloroaniline	19.7	61	26	171	93	85
			74	34	169	76	85
		24.6	67	25	181	90	92*
			60	20	171	95	92*
		30.7	65	17	100	51	68
			75	19	193	85	68
		38.4	43	9	172	132	120*
			61	12	197	107	120*
		48	64	9	225	117	119*
			55	8	196	120	119*
Positive Control	3-Methylcholanthrene	5	43	28	459	354	242*
			66	44	372	188
			74	47	406	182

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination