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Study Information
Study Attribute Value
CEBS Accession Number: 002-03161-0018-0000-2
Chemical Name: p-Chloroaniline
CASRN: 106-47-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G75586
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 120 101 32 41
    66 81 105 53
    90 110 104 38
    71 89 85 40
Test Chemical p-Chloroaniline 
31.3       
 115 117 81 24
    134r 133 107 27
  
62.5       
 85 86 108 42 36
    80 91 73 30
  
125         
 78 82 87 37
    132r 124 114 29
  
250         
 103 71 141 46 51
    75 53 127 56
  
375         
 68 14 202 100 80*
    109 24 196 60
  
500         
 81 12 244 100
   
  
1000         
   
Positive Control Ethyl Methane Sulfonate 
500         
 47 37 921 658 645*
    52 37 983 632

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 121r 108 74 20 28
    101 105 90 30
    105 95 85 27
    127r 108 78 21
Test Chemical p-Chloroaniline 
125         
 106 79 88 28 29
    106 96 99 31
  
250         
 83 46 74 30 29
    118 54 96 27
  
375         
 79 21 73 31 36
    95 27 115 40
  
425         
 89 18 123 46 45
    90 20 122 45
  
500         
 82 3 167 68 64*
    80# 7 144 60
  
600         
   
Positive Control Ethyl Methane Sulfonate 
500         
 74 43 911 413 447*
    65 42 936 480

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 87 53 22 16
    81 94 31 13
    85 108 32 13
    98 111 45 15
Test Chemical p-Chloroaniline 
200         
 38 46 20 18 19
    54 50 33 20
  
300         
 62 16 40 22 24
    51 35 48 32
    43 35 23 18
  
400         
 40 13 31 26 23
    76 7 49 21
    58 28 37 21
  
450         
 54 24 37 23 26*
    86 31 73 28
    83 22 70 28
  
500         
 100 22 77 26 25*
    89 15 62 23
    77 17 62 27
  
550         
 85 12 78 31 27*
    86 9 64 25
    54 22 42 26
Positive Control Ethyl Methane Sulfonate 
500         
 22 28 277 420 378*
    39 27 406 348
    41 24 455 367

Nonactivation Trial: 4 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 96 34 17 15
    66 105 21 11
    68 111 30 15
    62 88 36 19
Test Chemical p-Chloroaniline 
100         
 50 48 57 38 34*
    52 54 50 32
    42 47 42 34
  
200         
 39 39 35 30 31*
    44 30 44 34
    48 28 44 31
  
300         
 31 8 28 30 32*
    29 12 24 28
    29 12 32 37
  
400         
 35*
    42 5 31 25
    29 5 40 45
  
500         
   
    50 6 43 29
  
550         
    38 4 30 26
   
  
600         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 7r 4 436 2180 1075*
    13 10 466 1195
    19 12 535 955

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 96 96 37 37
    81 111 95 39
    65# 101 63 33
    75 91 84 37
Test Chemical p-Chloroaniline 
7.8       
 62 65 113 61 55
    68 69 100 49
  
15.6       
 78 63 144 62 65*
    70 48 145 69
  
31.3       
 83# 51 131 53 63*
    51 35 114 74
  
62.5       
 66 33 132 66 77*
    82 28 216 88
  
125         
 63 21 188 99 102*
    60 21 189 106
Positive Control 3-Methylcholanthrene 
5         
 39# 18 372 322 353*
    37 21 421 384

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 100 108 43 45
    78 112 107 46
    72 98 92 43
    88 90 128 48
Test Chemical p-Chloroaniline 
31.3       
 85 40 140 55 51
    73 34 104 47
  
62.5       
 72 23 172 80 68
    78 30 130 56
  
83.4       
 87 21 163 63 65
    70 21 140 66
  
125         
 79 17 206 87 88*
    66 15 174 88
  
166.7       
 67 15 119 59 67
    83 15 186 75
Positive Control 3-Methylcholanthrene 
5         
 41 29 317 260 244*
    55 24 378 228

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 108 55 21 22
    71 83 48 23
    102 94 74 24
    89 115 51 19
Test Chemical p-Chloroaniline 
75         
 84 30 93 37 39*
    80 39 102 43
    85 36 92 36
  
100         
 81 33 79 33 41*
    65 25 80 41
    78# 29 112.5 48
  
125         
 80 27 123 51 55*
    79 31 125 53
    91 29 162 60
  
150         
 90 25 124 46 50*
    90 23 133 49
    85 22 141 55
  
166.7       
 67 17 111 55 50*
    75 16 98 44
    86# 13 135 52
  
200         
 58 15 116 66 61*
    71 19 137 65
    68 17 103 51
Positive Control 3-Methylcholanthrene 
5         
 55 46 324 195 187*
    54 37 245 150
    47 36 305 216

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination