Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03161-0018-0000-2
Chemical Name:	p-Chloroaniline
CASRN:	106-47-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G75586
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	120	101	32	41
			66	81	105	53
			90	110	104	38
			71	89	85	40
Test Chemical	p-Chloroaniline	31.3	115	117	81	24
			134r	133	107	27
		62.5	85	86	108	42	36
			80	91	73	30	36
		125	78	82	87	37
			132r	124	114	29
		250	103	71	141	46	51
			75	53	127	56	51
		375	68	14	202	100	80*
			109	24	196	60	80*
		500	81	12	244	100

		1000

Positive Control	Ethyl Methane Sulfonate	500	47	37	921	658	645*
Positive Control			52	37	983	632	645*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	121r	108	74	20	28
			101	105	90	30
			105	95	85	27
			127r	108	78	21
Test Chemical	p-Chloroaniline	125	106	79	88	28	29
			106	96	99	31	29
		250	83	46	74	30	29
			118	54	96	27	29
		375	79	21	73	31	36
			95	27	115	40	36
		425	89	18	123	46	45
			90	20	122	45	45
		500	82	3	167	68	64*
			80#	7	144	60	64*
		600

Positive Control	Ethyl Methane Sulfonate	500	74	43	911	413	447*
Positive Control			65	42	936	480	447*

Nonactivation Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	87	53	22	16
			81	94	31	13
			85	108	32	13
			98	111	45	15
Test Chemical	p-Chloroaniline	200	38	46	20	18	19
			54	50	33	20	19
		300	62	16	40	22	24
			51	35	48	32
			43	35	23	18
		400	40	13	31	26	23
			76	7	49	21
			58	28	37	21
		450	54	24	37	23	26*
			86	31	73	28
			83	22	70	28
		500	100	22	77	26	25*
			89	15	62	23
			77	17	62	27
		550	85	12	78	31	27*
			86	9	64	25
			54	22	42	26
Positive Control	Ethyl Methane Sulfonate	500	22	28	277	420	378*
			39	27	406	348
			41	24	455	367

Nonactivation Trial: 4 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	96	34	17	15
			66	105	21	11
			68	111	30	15
			62	88	36	19
Test Chemical	p-Chloroaniline	100	50	48	57	38	34*
			52	54	50	32
			42	47	42	34
		200	39	39	35	30	31*
			44	30	44	34
			48	28	44	31
		300	31	8	28	30	32*
			29	12	24	28
			29	12	32	37
		400					35*
			42	5	31	25
			29	5	40	45
		500

			50	6	43	29
		550
			38	4	30	26

		600


Positive Control	Ethyl Methane Sulfonate	500	7r	4	436	2180	1075*
			13	10	466	1195
			19	12	535	955

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	96	96	37	37
			81	111	95	39
			65#	101	63	33
			75	91	84	37
Test Chemical	p-Chloroaniline	7.8	62	65	113	61	55
			68	69	100	49	55
		15.6	78	63	144	62	65*
			70	48	145	69	65*
		31.3	83#	51	131	53	63*
			51	35	114	74	63*
		62.5	66	33	132	66	77*
			82	28	216	88	77*
		125	63	21	188	99	102*
			60	21	189	106	102*
Positive Control	3-Methylcholanthrene	5	39#	18	372	322	353*
Positive Control			37	21	421	384	353*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	84	100	108	43	45
			78	112	107	46
			72	98	92	43
			88	90	128	48
Test Chemical	p-Chloroaniline	31.3	85	40	140	55	51
			73	34	104	47	51
		62.5	72	23	172	80	68
			78	30	130	56	68
		83.4	87	21	163	63	65
			70	21	140	66	65
		125	79	17	206	87	88*
			66	15	174	88	88*
		166.7	67	15	119	59	67
			83	15	186	75	67
Positive Control	3-Methylcholanthrene	5	41	29	317	260	244*
Positive Control			55	24	378	228	244*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	108	55	21	22
			71	83	48	23
			102	94	74	24
			89	115	51	19
Test Chemical	p-Chloroaniline	75	84	30	93	37	39*
			80	39	102	43
			85	36	92	36
		100	81	33	79	33	41*
			65	25	80	41
			78#	29	112.5	48
		125	80	27	123	51	55*
			79	31	125	53
			91	29	162	60
		150	90	25	124	46	50*
			90	23	133	49
			85	22	141	55
		166.7	67	17	111	55	50*
			75	16	98	44
			86#	13	135	52
		200	58	15	116	66	61*
			71	19	137	65
			68	17	103	51
Positive Control	3-Methylcholanthrene	5	55	46	324	195	187*
			54	37	245	150
			47	36	305	216

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination