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Study Information
Study Attribute Value
CEBS Accession Number: 002-03176-0001-0000-0
Chemical Name: p-Nitroaniline
CASRN: 100-01-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 491256_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/08/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1037 428 0.413 8.56 26.0 0.000
Negative (Not Specified) 
0         
 50 1040 381 0.366 7.62 26.0 -11.238
Test Chemical p-Nitroaniline 
 0.5       
 50 1027 392 0.382 7.84 26.0 -7.519 
1.6       
 50 1027 414 0.403 8.28 26.0 -2.329 
5         
 50 1035 443 0.428 8.86 26.0 3.705 
16         
 50 1027 415 0.404 8.30 26.0 -2.093 
50         
 50 1032 445 0.431 8.90 26.0 4.476 
160         
 50 1046 571 0.546 11.42 26.0 32.263
Positive Control Mitomycin-C 
 0.01      
 50 1036 1761 1.700 35.22 26.0 311.846
Trend: 4.656        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/10/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 381 0.365 7.62 26.0 -20.688
Dimethyl Sulfoxide 
0         
 50 1041 479 0.460 9.58 26.0 0.000
Test Chemical p-Nitroaniline 
50         
 50 1040 440 0.423 8.80 26.0 -8.054 
100         
 50 1037 485 0.468 9.70 26.0 1.643 
200         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1050 1393 1.327 27.86 26.0 188.322
Mitomycin-C 
 0.01      
 50 1045 2049 1.961 40.98 26.0 326.129
Trend: 0.255        
Probability: 0.399        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/08/1982    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 505 0.485 10.10 26.0 0.895
Dimethyl Sulfoxide 
0         
 50 1043 501 0.480 10.02 26.0 0.000
Test Chemical p-Nitroaniline 
16         
 50 1045 472 0.452 9.44 26.0 -5.969 
50         
 50 1040 466 0.448 9.32 26.0 -6.718 
160         
 50 1036 458 0.442 9.16 26.0 -7.965 
500         
 50 1036 460 0.444 9.20 26.0 -7.563 
1600         
 50 1049 563 0.537 11.26 26.0 11.732 
5000         
 50 1031 550 0.533 11.00 26.0 11.058
Positive Control Cyclophosphamide 
2         
 50 1045 2671 2.556 53.42 26.0 432.113
Trend: 2.875        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/10/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 387 0.369 7.74 26.0 -11.000
Dimethyl Sulfoxide 
0         
 50 1046 434 0.415 8.68 26.0 0.000
Test Chemical p-Nitroaniline 
160         
 50 1048 419 0.400 8.38 26.0 -3.640 
500         
 50 1048 436 0.416 8.72 26.0 0.269 
1000         
 0 0 0 0.000 0.00 26.0 N/A 
2000         
 0 0 0 0.000 0.00 26.0 N/A 
3000         
 0 0 0 0.000 0.00 26.0 N/A 
4000         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1044 1894 1.814 37.88 26.0 337.242
Cyclophosphamide 
2         
 50 1049 3083 2.939 61.66 26.0 608.337
Trend: 0.142        
Probability: 0.443        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 10/08/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 513 0.492 10.26 26.0 0.000
Negative (Not Specified) 
0         
 50 1037 461 0.445 9.22 26.0 -9.617
Test Chemical p-Nitroaniline 
250         
 50 1038 508 0.489 10.16 31.0 -0.498 
500         
 50 1044 540 0.517 10.80 31.0 5.162 
750         
 50 1029 604 0.587 12.08 31.0 19.341 
1000         
 50 1039 566 0.545 11.32 31.0 10.756
Positive Control Cyclophosphamide 
2         
 50 1043 2345 2.248 46.90 26.0 357.115
Trend: 2.969        
Probability: 0.001