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Study Information
Study Attribute Value
CEBS Accession Number: 002-03176-0002-0000-1
Chemical Name: p-Nitroaniline
CASRN: 100-01-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 491256_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Questionable 
Induced Rat Liver S9  4_S9  Weakly Positive* 
Induced Rat Liver S9  5_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 04/08/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
16         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 24 0.240 19.0 11 0.110 10.0 6 0.060 6.0 7 0.070 6.0
Trend: 1.415 1.415 0.000  
Probability: 0.079 0.079 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/08/1982    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
16         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
160         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
500         
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 0.0 
1600         
 100 11 0.110 11.0 0 0.000 0.0 4 0.040 4.0 7 0.070 0.0
Positive Control: Cyclophosphamide 
50         
 100 136 1.360 61.0 50 0.500 38.0 32 0.320 26.0 54 0.540 24.0
Trend: 4.488 0.505 2.562  
Probability: 0.000 0.307 0.005  


Trial #:2   Activation: No Activation    Date: 08/22/1982    Harvest Time: 12.8 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
100         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
600         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
800         
 100 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 
1200         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 33 0.330 28.0 13 0.130 12.0 15 0.150 15.0 5 0.050 5.0
Trend: 1.026 0.725 -0.328  
Probability: 0.152 0.234 0.628  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 10/09/1982    Harvest Time: 22 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
400         
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 1.0 0 0.000 0.0 
600         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
800         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
1200         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
1600         
 100 120 1.200 73.0 21 0.210 16.0 91 0.910 65.0 8 0.080 6.0 
2000         
 100 77 0.770 63.0 18 0.180 18.0 54 0.540 48.0 5 0.050 5.0
Positive Control: Cyclophosphamide 
50         
 100 209 2.090 90.0 78 0.780 67.0 98 0.980 39.0 33 0.330 26.0
Trend: 14.671 6.389 13.369  
Probability: 0.000 0.000 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 08/22/1982    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 1.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
200         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
400         
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
600         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
800         
 100 5 0.050 4.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
1200         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1600         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 183 1.830 71.0 50 0.500 38.0 58 0.580 41.0 75 0.750 43.0
Trend: 1.992 1.670 1.548  
Probability: 0.023 0.047 0.061  


Trial #:4_S9   Activation: Induced Rat Liver S9    Date: 08/22/1982    Harvest Time: 15 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
400         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
600         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
800         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1200         
 100 60 0.600 42.0 6 0.060 6.0 37 0.370 30.0 17 0.170 16.0
Positive Control: Cyclophosphamide 
50         
 100 183 1.830 71.0 50 0.500 38.0 58 0.580 41.0 75 0.750 43.0
Trend: 8.960 2.989 7.473  
Probability: 0.000 0.001 0.000  


Trial #:5_S9   Activation: Induced Rat Liver S9    Date: 09/30/1982    Harvest Time: 12.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: p-Nitroaniline 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
600         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
800         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
1000         
 100 81 0.810 74.0 1 0.010 1.0 42 0.420 37.0 38 0.380 38.0
Positive Control: Cyclophosphamide 
25         
 100 76 0.760 45.0 23 0.230 15.0 45 0.450 36.0 8 0.080 8.0
Trend: 11.234 1.285 7.082  
Probability: 0.000 0.099 0.000