Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03176-0011-0000-1
Chemical Name:	p-Nitroaniline
CASRN:	100-01-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	368298
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	107	106	135	42	48
			122r	129	160	44
			146r	118	205	47
			101	94	164	54
Test Chemical	p-Nitroaniline	15.6	91	69	157	58	50
			105#	74	152	48
			101#	78	132	44
		31.3	99#	91	140	47	50
			108	87	175	54
			98	81	147	50
		62.5	126r	123	131	35
			102	96	128	42
		125	107	87	163	51	54
			97	65	167	57
			129r	112	161	42
		250	111#	80	139	42	51
			102	68	184	60
			130r	88	169	44
		500	124r	42	273	73	137*
			105#	10	531	169
			91	32	285	105
		1000


Positive Control	Ethyl Methane Sulfonate	250	70	67	997	475	421*
			99	67	1242	418
			89#	56	988	369

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	102	104	62	20	23
			92	124	60	22
			104	79	85	27
			72	94	46	21
Test Chemical	p-Nitroaniline	15.6	74	81	62	28	35*
			57#	62	64	38
			75	54	88	39
		31.3	59	58	40	23	28
			58	64	56	32
			78	80	70	30
		62.5	81	69	61	25	31
			59	52	70	39
			57	60	49	29
		125	84	47	71	28	34
			66	58	57	29
			65	36	88	45
		250	68	26	135	66	81*
			71	21	192	90
			65	12	170	87
		500	63	8	217	115


		1000


Positive Control	Ethyl Methane Sulfonate	250	71	68	711	335	324*
			70	52	683	324
			68	60	644	314

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	76	99	46	20	33
			79	110	96	41
			72	97	96	44
			67#	94	56	28
Test Chemical	p-Nitroaniline	50	60	77	66	37	45
			58	64	106	61
			51	66	58	38
		100	89	85	91	34	29
			71	78	47	22
			72	78	65	30
		200	79	52	127	54	49
			80	57	150	63
			92	61	88	32
		300	54	16	146	90	96*
			52	20	195	125
			60	12	132	74
		400


Positive Control	Ethyl Methane Sulfonate	250	56	74	630	375	398*
			70	72	839	401
			64	71	803	417

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	125r	171	94	25	34
			88	106	78	30
			70	95	60	29
			67	99	86	43
Test Chemical	p-Nitroaniline	25	60	109	67	37	34
			68	111	69	34
			77	97	72	31
		50	64	96	98	51	47
			88	88	107	41
			73	83	108	49
		100	71	67	74	35	50
			62	55	104	56
			92	101	166	60
		200	70	82	99	47	47
			74	67	107	48
			71	70	96	45
		300	74	57	126	57	58*
			51	35	102	67
			79	48	122	51
		500	82	12	346	141	105*
			80	15	245	102
			77	53	162	71
Positive Control	3-Methylcholanthrene	2.5	118	100	507	144	159*
			91	101	464	170
			94	93	465	164

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination