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Study Information
Study Attribute Value
CEBS Accession Number: 002-03176-0011-0000-1
Chemical Name: p-Nitroaniline
CASRN: 100-01-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 368298
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 107 106 135 42 48
    122r 129 160 44
    146r 118 205 47
    101 94 164 54
Test Chemical p-Nitroaniline 
15.6       
 91 69 157 58 50
    105# 74 152 48
    101# 78 132 44
  
31.3       
 99# 91 140 47 50
    108 87 175 54
    98 81 147 50
  
62.5       
 126r 123 131 35
    102 96 128 42
  
125         
 107 87 163 51 54
    97 65 167 57
    129r 112 161 42
  
250         
 111# 80 139 42 51
    102 68 184 60
    130r 88 169 44
  
500         
 124r 42 273 73 137*
    105# 10 531 169
    91 32 285 105
  
1000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 70 67 997 475 421*
    99 67 1242 418
    89# 56 988 369

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 102 104 62 20 23
    92 124 60 22
    104 79 85 27
    72 94 46 21
Test Chemical p-Nitroaniline 
15.6       
 74 81 62 28 35*
    57# 62 64 38
    75 54 88 39
  
31.3       
 59 58 40 23 28
    58 64 56 32
    78 80 70 30
  
62.5       
 81 69 61 25 31
    59 52 70 39
    57 60 49 29
  
125         
 84 47 71 28 34
    66 58 57 29
    65 36 88 45
  
250         
 68 26 135 66 81*
    71 21 192 90
    65 12 170 87
  
500         
 63 8 217 115
   
   
  
1000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 71 68 711 335 324*
    70 52 683 324
    68 60 644 314

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 76 99 46 20 33
    79 110 96 41
    72 97 96 44
    67# 94 56 28
Test Chemical p-Nitroaniline 
50         
 60 77 66 37 45
    58 64 106 61
    51 66 58 38
  
100         
 89 85 91 34 29
    71 78 47 22
    72 78 65 30
  
200         
 79 52 127 54 49
    80 57 150 63
    92 61 88 32
  
300         
 54 16 146 90 96*
    52 20 195 125
    60 12 132 74
  
400         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 56 74 630 375 398*
    70 72 839 401
    64 71 803 417

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 125r 171 94 25 34
    88 106 78 30
    70 95 60 29
    67 99 86 43
Test Chemical p-Nitroaniline 
25         
 60 109 67 37 34
    68 111 69 34
    77 97 72 31
  
50         
 64 96 98 51 47
    88 88 107 41
    73 83 108 49
  
100         
 71 67 74 35 50
    62 55 104 56
    92 101 166 60
  
200         
 70 82 99 47 47
    74 67 107 48
    71 70 96 45
  
300         
 74 57 126 57 58*
    51 35 102 67
    79 48 122 51
  
500         
 82 12 346 141 105*
    80 15 245 102
    77 53 162 71
Positive Control 3-Methylcholanthrene 
2.5       
 118 100 507 144 159*
    91 101 464 170
    94 93 465 164

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination