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Study Information
Study Attribute Value
CEBS Accession Number: 002-03178-0002-0000-3
Chemical Name: p-Nitrobenzoic acid
CASRN: 62-23-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 195725_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 06/19/1985    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 7 0.035 3.0 1 0.005 0.5 6 0.030 2.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 8 0.040 3.0 2 0.010 1.0 5 0.025 2.0 1 0.005 0.5
Test Chemical: p-Nitrobenzoic acid 
875         
 200 21 0.105 8.5 5 0.025 2.5 15 0.075 6.5 1 0.005 0.5 
1313         
 200 30 0.150 12.5 3 0.015 1.5 27 0.135 11.5 0 0.000 0.0 
1750         
 50 20 0.400 26.0 1 0.020 2.0 19 0.380 24.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 50 22 0.440 30.0 5 0.100 8.0 17 0.340 24.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 37 1.480 56.0 11 0.440 36.0 25 1.000 48.0 1 0.040 4.0
Trend: 4.947 0.379 5.247  
Probability: 0.000 0.352 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/19/1985    Harvest Time: 12.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 7 0.035 3.0 0 0.000 0.0 7 0.035 3.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 10 0.050 3.5 1 0.005 0.5 7 0.035 3.0 2 0.010 1.0
Test Chemical: p-Nitrobenzoic acid 
875         
 200 13 0.065 5.5 5 0.025 2.5 8 0.040 3.5 0 0.000 0.0 
1313         
 200 14 0.070 5.5 1 0.005 0.5 11 0.055 4.5 2 0.010 1.0 
1750         
 200 24 0.120 9.0 5 0.025 2.0 17 0.085 6.5 2 0.010 1.0
Positive Control: Cyclophosphamide 
7.5       
 200 30 0.150 12.0 11 0.055 5.5 17 0.085 7.5 2 0.010 1.0
Cyclophosphamide 
37.5       
 25 38 1.520 84.0 10 0.400 32.0 28 1.120 80.0 0 0.000 0.0
Trend: 2.160 0.586 1.751  
Probability: 0.015 0.279 0.040  


Trial #:2   Activation: No Activation    Date: 08/21/1985    Harvest Time: 21.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: p-Nitrobenzoic acid 
439.5       
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0 
879         
 200 7 0.035 3.5 4 0.020 2.0 3 0.015 1.5 0 0.000 0.0 
1313         
 200 17 0.085 7.5 1 0.005 0.5 16 0.080 7.0 0 0.000 0.0 
1750         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 48 0.240 15.0 24 0.120 11.5 24 0.120 10.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 28 1.120 48.0 13 0.520 36.0 15 0.600 40.0 0 0.000 0.0
Trend: 4.034 1.277 3.876  
Probability: 0.000 0.101 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/22/1985    Harvest Time: 12.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 50 1 0.020 2.0 0 0.000 0.0 1 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: p-Nitrobenzoic acid 
1249         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
1505         
 200 10 0.050 4.5 1 0.005 0.5 9 0.045 4.0 0 0.000 0.0 
1750         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 16 0.080 7.0 3 0.015 1.5 13 0.065 6.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 12 0.480 36.0 5 0.200 20.0 7 0.280 24.0 0 0.000 0.0
Trend: 1.626 1.804 0.908  
Probability: 0.052 0.036 0.182