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Study Information
Study Attribute Value
CEBS Accession Number: 002-03184-0001-0000-9
Chemical Name: p-Nitrosodiphenylamine
CASRN: 156-10-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 976337_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/01/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 448 0.429 8.96 25.5 0.000
Test Chemical p-Nitrosodiphenylamine 
 0.125     
 50 1037 540 0.521 10.80 25.5 21.349 
 0.417     
 50 1041 624 0.599 12.48 31.3 39.687 
1.3       
 50 1040 630 0.606 12.60 31.3 41.166 
4.2       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1046 588 0.562 11.76 25.5 30.999
Mitomycin-C 
 0.01      
 5 105 200 1.905 40.00 25.5 343.878
Trend: 6.005        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/29/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 514 227 0.442 9.08 25.7 0.000
Test Chemical p-Nitrosodiphenylamine 
 0.51      
 25 520 242 0.465 9.68 25.7 5.378 
 0.75      
 25 511 272 0.532 10.88 31.4 20.527 
1.3       
 25 519 305 0.588 12.20 31.4 33.067 
2.5       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 25 519 310 0.597 12.40 25.7 35.248
Mitomycin-C 
 0.01      
 5 104 223 2.144 44.60 25.7 385.522
Trend: 3.579        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/01/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 450 0.430 9.00 25.5 0.000
Test Chemical p-Nitrosodiphenylamine 
12.5       
 50 1047 473 0.452 9.46 25.5 5.011 
41.7       
 50 1038 690 0.665 13.80 25.5 54.515 
125         
 50 1039 560 0.539 11.20 25.5 25.283 
416.7       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1048 606 0.578 12.12 25.5 34.410
Cyclophosphamide 
2         
 5 105 123 1.171 24.60 25.5 172.292
Trend: 5.507        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 10/29/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 527 226 0.429 9.04 25.7 0.000
Test Chemical p-Nitrosodiphenylamine 
24.9       
 25 521 414 0.795 16.56 25.7 85.295 
37.7       
 25 524 297 0.567 11.88 31.4 32.168 
50.3       
 25 522 306 0.586 12.24 31.4 36.695 
125.7       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 25 524 337 0.643 13.48 25.7 49.969
Cyclophosphamide 
2         
 5 105 165 1.571 33.00 25.7 266.435
Trend: 2.322        
Probability: 0.010