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Study Information
Study Attribute Value
CEBS Accession Number: 002-03184-0007-0000-5
Chemical Name: p-Nitrosodiphenylamine
CASRN: 156-10-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 722133
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 112 53 21 21
    86 111 54 21
    80 92 62 26
    78 85 43 18
Test Chemical p-Nitrosodiphenylamine 
 0.25      
 79 77 62 26 26
    65 79 49 25
    62 64 47 25
  
 0.5       
 86 66 69 27 35*
    74# 57 74 33
    65 40 89 46
  
 0.75      
 57 37 68 40 40*
    54# 28 58.5 36
    60 40 79 44
  
1         
 29# 4 114 131 164*
    12 1 102 287
    38 5 84 74
  
1.25      
   
    37 2 107 97
  
1.5       
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 5r 1 156 1156
    7r 1 279 1431
    5r 1 232 1657

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 98 68 24 18
    86# 101 40.5 16
    119# 114 43.5 12
    92# 87 56 20
Test Chemical p-Nitrosodiphenylamine 
 0.25      
 72 72 37 17 15
    79 79 28 12
  
 0.5       
 83 49 51 20 18
    68 47 42 21
    87# 59 33 13
  
 0.75      
 69 45 75 36 29*
    64 51 54 28
    79# 58 54 23
  
1         
 67 22 74 37 46*
    73# 17 100 46
    64 14 101 53
    60# 17 88 49
  
1.25      
 56 4 150 89 85*
    50 13 113 75
    42 2 115 92
  
1.5       
   
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 66# 53 670.5 339 355*
    55 53 612 371

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 95 53 22 25
    93 103 96 34
    97 113 58 20
    83 88 58 23
Test Chemical p-Nitrosodiphenylamine 
 0.25      
 109 99 89 27 25
    87 60 60 23
  
 0.5       
 87 57 89 34 55*
    70 29 116 55
    76# 31 172.5 75
  
 0.75      
 63 24 89 47 126*
    46 4 330 241
    54 13 148 91
  
1         
   
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 64 50 783 410 361*
    87 63 736 283
    65 50 754 390

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 107 98 149 47 48
    86 94 137 53
    71# 92 96 45
    100 116 140 47
Test Chemical p-Nitrosodiphenylamine 
 0.125     
 81 89 67 28 33
    112 104 127 38
  
 0.25      
 92 79 86 31 39
    91 97 127 46
  
 0.5       
 98# 85 121.5 41 42
    99 88 129 44
  
1         
 80 80 119 49 45
    85 70 101 40
  
2         
 80 61 120 50 41
    98 70 95 32
  
4         
 96 45 164 57 52
    86 39 121 47
Positive Control 3-Methylcholanthrene 
5         
 30 4 321 357 309*
    34 5 241 240
    28 5 280 331

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 84 151 64 55
    76 119 111 49
    74 97 117 53
Test Chemical p-Nitrosodiphenylamine 
 0.5       
 45 62 77 57 53
    45 63 83 61
    57 78 67 39
  
1         
 64 65 136 71 57
    56 88 76 45
    49# 64 81 55
  
2         
 62 92 76 41 40
    68# 73 99 49
    61 64 57 31
  
4         
 64 59 44 23 37
    51 33 81 53
    60 59 64 35
  
6         
 58 29 69 40 45
    63 31 93 50
    71 44 97 46
  
8         
 63 20 99 53 59
    63 17 102 54
   
    78# 14 168 72
  
10         
   
   
   
Positive Control 3-Methylcholanthrene 
5         
 14 2 182 449 391*
    23 3 198 289
    17# 2 226.5 436

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination