Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03184-0007-0000-5
Chemical Name:	p-Nitrosodiphenylamine
CASRN:	156-10-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	722133
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	112	53	21	21
			86	111	54	21
			80	92	62	26
			78	85	43	18
Test Chemical	p-Nitrosodiphenylamine	0.25	79	77	62	26	26
			65	79	49	25
			62	64	47	25
		0.5	86	66	69	27	35*
			74#	57	74	33
			65	40	89	46
		0.75	57	37	68	40	40*
			54#	28	58.5	36
			60	40	79	44
		1	29#	4	114	131	164*
			12	1	102	287
			38	5	84	74
		1.25

			37	2	107	97
		1.5


Positive Control	Ethyl Methane Sulfonate	500	5r	1	156	1156
			7r	1	279	1431
			5r	1	232	1657

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	98	68	24	18
			86#	101	40.5	16
			119#	114	43.5	12
			92#	87	56	20
Test Chemical	p-Nitrosodiphenylamine	0.25	72	72	37	17	15
			79	79	28	12	15
		0.5	83	49	51	20	18
			68	47	42	21
			87#	59	33	13
		0.75	69	45	75	36	29*
			64	51	54	28
			79#	58	54	23
		1	67	22	74	37	46*
			73#	17	100	46
			64	14	101	53
			60#	17	88	49
		1.25	56	4	150	89	85*
			50	13	113	75
			42	2	115	92
		1.5



Positive Control	Ethyl Methane Sulfonate	250	66#	53	670.5	339	355*
Positive Control			55	53	612	371	355*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	95	53	22	25
			93	103	96	34
			97	113	58	20
			83	88	58	23
Test Chemical	p-Nitrosodiphenylamine	0.25	109	99	89	27	25
			87	60	60	23	25
		0.5	87	57	89	34	55*
			70	29	116	55
			76#	31	172.5	75
		0.75	63	24	89	47	126*
			46	4	330	241
			54	13	148	91
		1



Positive Control	Ethyl Methane Sulfonate	250	64	50	783	410	361*
			87	63	736	283
			65	50	754	390

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	107	98	149	47	48
			86	94	137	53
			71#	92	96	45
			100	116	140	47
Test Chemical	p-Nitrosodiphenylamine	0.125	81	89	67	28	33
			112	104	127	38	33
		0.25	92	79	86	31	39
			91	97	127	46	39
		0.5	98#	85	121.5	41	42
			99	88	129	44	42
		1	80	80	119	49	45
			85	70	101	40	45
		2	80	61	120	50	41
			98	70	95	32	41
		4	96	45	164	57	52
			86	39	121	47	52
Positive Control	3-Methylcholanthrene	5	30	4	321	357	309*
			34	5	241	240
			28	5	280	331

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	84	151	64	55
			76	119	111	49
			74	97	117	53
Test Chemical	p-Nitrosodiphenylamine	0.5	45	62	77	57	53
			45	63	83	61
			57	78	67	39
		1	64	65	136	71	57
			56	88	76	45
			49#	64	81	55
		2	62	92	76	41	40
			68#	73	99	49
			61	64	57	31
		4	64	59	44	23	37
			51	33	81	53
			60	59	64	35
		6	58	29	69	40	45
			63	31	93	50
			71	44	97	46
		8	63	20	99	53	59
			63	17	102	54

			78#	14	168	72
		10



Positive Control	3-Methylcholanthrene	5	14	2	182	449	391*
			23	3	198	289
			17#	2	226.5	436

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination