Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03184-0008-0000-6
Chemical Name:	p-Nitrosodiphenylamine
CASRN:	156-10-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	986345
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	99	130	56	48
			80	112	109	46
			76#	91	121	53
			82#	98	93	38
Test Chemical	p-Nitrosodiphenylamine	0.5	77	72	111	48	47
			80	82	110	46	47
		0.7	52	44	116	75	62
			72	57	104	48	62
		1	47	13	204	144	135*
			46	14	173	126	135*
		1.4

Positive Control	Ethyl Methane Sulfonate	500	42	39	758	597	569*
			37	36	713	642
			53	52	747	468

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	50	98	19	13	13
			47r	85	11	8
			55	102	21	13
			44r	79	44	33
Test Chemical	p-Nitrosodiphenylamine	0.42	38	54	12	11	17
			35	47	24	23	17
		0.52	37	42	26	24	29*
			39	46	40	34	29*
		0.66
			31	21	37	40
		0.82	36	23	32	30	31*
			33	17	32	32	31*
		1.02

Positive Control	Ethyl Methane Sulfonate	500	32	42	445	468	523*
			27	33	429	526
			27	33	465	574

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	89	91	41	41
			72	97	73	34
			87	116	144	55
			78	98	84	36
Test Chemical	p-Nitrosodiphenylamine	2.8	95	140	130	46	39
			90	129	87	32	39
		4	96	128	101	35	31
			91	132	76	28	31
		5.7	76	91	120	52	44
			94	122	100	36	44
		8.2	93	84	137	49	56
			88	80	165	63	56
		11.7	83	52	141	57	60
			76	42	144	63	60
		16.7	42	5	232	184

		23.8

Positive Control	3-Methylcholanthrene	5	61	76	361	197	215*
			61	73	365	198
			60	68	445	249

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	114	17	9	12
			47r	83	34	24
			42r	75	25	20
			54	86	25	16
Test Chemical	p-Nitrosodiphenylamine	3.9	55	82	48	29	23
			44	63	22	17	23
		4.9	46	57	35	26	33*
			52	68	63	40	33*
		6.1	48	54	39	27	30*
			42	44	41	33	30*
		7.7	51	39	29	19	24*
			45	39	40	30	24*
		9.6	43	29	28	22	26*
			45	24	42	31	26*
		12	50	17	52	35	36*
			42	16	46	36	36*
Positive Control	3-Methylcholanthrene	5	26	19	248	314	357*
			24	17	287	404
			23	16	243	352

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination