Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-03184-0008-0000-6
Chemical Name: p-Nitrosodiphenylamine
CASRN: 156-10-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 986345
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 99 130 56 48
    80 112 109 46
    76# 91 121 53
    82# 98 93 38
Test Chemical p-Nitrosodiphenylamine 
 0.5       
 77 72 111 48 47
    80 82 110 46
  
 0.7       
 52 44 116 75 62
    72 57 104 48
  
1         
 47 13 204 144 135*
    46 14 173 126
  
1.4       
   
Positive Control Ethyl Methane Sulfonate 
500         
 42 39 758 597 569*
    37 36 713 642
    53 52 747 468

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 50 98 19 13 13
    47r 85 11 8
    55 102 21 13
    44r 79 44 33
Test Chemical p-Nitrosodiphenylamine 
 0.42      
 38 54 12 11 17
    35 47 24 23
  
 0.52      
 37 42 26 24 29*
    39 46 40 34
  
 0.66      
    31 21 37 40
  
 0.82      
 36 23 32 30 31*
    33 17 32 32
  
1.02      
   
Positive Control Ethyl Methane Sulfonate 
500         
 32 42 445 468 523*
    27 33 429 526
    27 33 465 574

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 89 91 41 41
    72 97 73 34
    87 116 144 55
    78 98 84 36
Test Chemical p-Nitrosodiphenylamine 
2.8       
 95 140 130 46 39
    90 129 87 32
  
4         
 96 128 101 35 31
    91 132 76 28
  
5.7       
 76 91 120 52 44
    94 122 100 36
  
8.2       
 93 84 137 49 56
    88 80 165 63
  
11.7       
 83 52 141 57 60
    76 42 144 63
  
16.7       
 42 5 232 184
   
  
23.8       
   
Positive Control 3-Methylcholanthrene 
5         
 61 76 361 197 215*
    61 73 365 198
    60 68 445 249

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 114 17 9 12
    47r 83 34 24
    42r 75 25 20
    54 86 25 16
Test Chemical p-Nitrosodiphenylamine 
3.9       
 55 82 48 29 23
    44 63 22 17
  
4.9       
 46 57 35 26 33*
    52 68 63 40
  
6.1       
 48 54 39 27 30*
    42 44 41 33
  
7.7       
 51 39 29 19 24*
    45 39 40 30
  
9.6       
 43 29 28 22 26*
    45 24 42 31
  
12         
 50 17 52 35 36*
    42 16 46 36
Positive Control 3-Methylcholanthrene 
5         
 26 19 248 314 357*
    24 17 287 404
    23 16 243 352

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination