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Study Information
Study Attribute Value
CEBS Accession Number: 002-03187-0002-0000-3
Chemical Name: p-Phenylenediamine dihydrochloride
CASRN: 624-18-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 921738_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Weakly Positive* 
Induced Rat Liver S9  2_S9  Questionable 

Trial #:1   Activation: No Activation    Date: 11/16/1983    Harvest Time: 12.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Medium 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: p-Phenylenediamine dihydrochloride 
5         
 100 4 0.040 0.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
16         
 100 7 0.070 6.0 4 0.040 3.0 3 0.030 3.0 0 0.000 0.0 
30         
 100 15 0.150 12.0 3 0.030 2.0 12 0.120 10.0 0 0.000 0.0 
50         
 100 51 0.510 35.0 23 0.230 20.0 27 0.270 19.0 1 0.010 1.0 
100         
 15 10 0.667 53.0 4 0.267 27.0 6 0.400 33.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 81 0.810 46.0 47 0.470 34.0 34 0.340 26.0 0 0.000 0.0
Trend: 8.168 6.110 5.077  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/16/1983    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Medium 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: p-Phenylenediamine dihydrochloride 
250         
 100 16 0.160 11.0 8 0.080 6.0 8 0.080 7.0 0 0.000 0.0 
500         
 100 29 0.290 10.0 9 0.090 5.0 10 0.100 7.0 10 0.100 1.0 
1000         
 100 41 0.410 23.0 20 0.200 13.0 19 0.190 15.0 2 0.020 2.0 
2000         
 40 29 0.725 43.0 11 0.275 15.0 14 0.350 20.0 4 0.100 10.0
Positive Control: Cyclophosphamide 
50         
 100 172 1.720 78.0 84 0.840 52.0 86 0.860 50.0 2 0.020 2.0
Trend: 6.083 3.388 4.272  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 09/20/1983    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Medium 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: p-Phenylenediamine dihydrochloride 
5         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
16         
 100 24 0.240 23.0 5 0.050 5.0 19 0.190 19.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 105 1.050 59.0 48 0.480 37.0 57 0.570 40.0 0 0.000 0.0
Trend: 5.307 2.163 4.966  
Probability: 0.000 0.015 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/20/1983    Harvest Time: 14 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: p-Phenylenediamine dihydrochloride 
5         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
16         
 100 10 0.100 9.0 4 0.040 4.0 6 0.060 5.0 0 0.000 0.0 
50         
 100 10 0.100 8.0 2 0.020 2.0 7 0.070 7.0 1 0.010 1.0 
160         
 100 6 0.060 5.0 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 
500         
 100 4 0.040 4.0 4 0.040 4.0 0 0.000 0.0 0 0.000 0.0 
1000         
 100 33 0.330 12.0 10 0.100 8.0 12 0.120 7.0 11 0.110 2.0
Positive Control: Cyclophosphamide 
50         
 100 103 1.030 56.0 48 0.480 34.0 52 0.520 40.0 3 0.030 3.0
Trend: 2.100 3.057 0.698  
Probability: 0.018 0.001 0.243