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Study Information
Study Attribute Value
CEBS Accession Number: 002-03205-0002-0000-4
Chemical Name: t-Butylhydroquinone
CASRN: 1948-33-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 089956_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Test Failure 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 05/28/1986    Harvest Time: 10 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: t-Butylhydroquinone 
5         
 200 6 0.030 3.0 2 0.010 1.0 4 0.020 2.0 0 0.000 0.0 
7.6       
 200 5 0.025 2.5 4 0.020 2.0 1 0.005 0.5 0 0.000 0.0 
10.1       
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 
25.2       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 200 37 0.185 16.0 16 0.080 8.0 21 0.105 9.5 0 0.000 0.0
Mitomycin-C 
 0.75      
 25 13 0.520 32.0 7 0.280 28.0 6 0.240 16.0 0 0.000 0.0
Trend: 0.340 0.882 -0.404  
Probability: 0.367 0.189 0.657  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 05/28/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: t-Butylhydroquinone 
149.4       
 25 22 0.880 44.0 10 0.400 28.0 12 0.480 32.0 0 0.000 0.0 
199.2       
 10 31 3.100 90.0 14 1.400 80.0 17 1.700 90.0 0 0.000 0.0 
249         
 10 39 3.900 90.0 13 1.300 80.0 26 2.600 90.0 0 0.000 0.0 
300         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 22 0.220 18.0 7 0.070 6.0 15 0.150 12.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 20 0.800 48.0 8 0.320 20.0 12 0.480 32.0 0 0.000 0.0
Trend: 9.280 8.685 9.617  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 04/29/1986    Harvest Time: 10 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: t-Butylhydroquinone 
25         
 200 8 0.040 3.0 4 0.020 1.5 4 0.020 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.75      
 25 14 0.560 36.0 8 0.320 20.0 6 0.240 20.0 0 0.000 0.0
Trend: 0.000 0.000 0.000  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 04/29/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 11 0.055 2.0 1 0.005 0.5 10 0.050 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 19 0.095 4.5 4 0.020 2.0 3 0.015 1.0 12 0.060 1.5
Test Chemical: t-Butylhydroquinone 
100.5       
 100 79 0.790 36.0 33 0.330 23.0 36 0.360 24.0 10 0.100 1.0 
150         
 20 53 2.650 75.0 23 1.150 65.0 20 1.000 50.0 10 0.500 5.0 
200         
 10 36 3.600 80.0 15 1.500 70.0 11 1.100 50.0 10 1.000 10.0
Positive Control: Cyclophosphamide 
6.25      
 200 43 0.215 20.0 17 0.085 8.0 24 0.120 11.5 2 0.010 1.0
Cyclophosphamide 
12.5       
 25 22 0.880 40.0 6 0.240 24.0 6 0.240 24.0 10 0.400 4.0
Trend: 10.109 9.782 8.542  
Probability: 0.000 0.000 0.000