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Study Information
Study Attribute Value
CEBS Accession Number: 002-03206-0001-0000-4
Chemical Name: tert-Butyl alcohol
CASRN: 75-65-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 263726_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/31/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 441 0.424 8.82 26.0 5.251
Medium 
0         
 50 1039 419 0.403 8.38 26.0 0.000
Test Chemical tert-Butyl alcohol 
160         
 50 1045 444 0.425 8.88 26.0 5.358 
500         
 50 1047 457 0.436 9.14 26.0 8.236 
1600         
 50 1046 486 0.465 9.72 26.0 15.214 
5000         
 50 1049 509 0.485 10.18 26.0 20.322
Positive Control Mitomycin-C 
 0.001     
 50 1039 661 0.636 13.22 26.0 57.757
Mitomycin-C 
 0.01      
 10 209 556 2.660 55.60 26.0 559.675
Trend: 3.128        
Probability: 0.001        

Trial #: 2    Activation: No Activation    Date: 03/08/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 366 0.352 7.32 26.0 -14.802
Medium 
0         
 50 1040 430 0.413 8.60 26.0 0.000
Test Chemical tert-Butyl alcohol 
2000         
 50 1037 437 0.421 8.74 26.0 1.922 
3000         
 50 1039 433 0.417 8.66 26.0 0.795 
4000         
 50 1046 478 0.457 9.56 26.0 10.525 
5000         
 50 1040 453 0.436 9.06 26.0 5.349
Positive Control Mitomycin-C 
 0.001     
 50 1048 1287 1.228 25.74 26.0 197.018
Mitomycin-C 
 0.01      
 10 210 674 3.210 67.40 26.0 676.257
Trend: 1.261        
Probability: 0.104        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/31/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 373 0.359 7.46 26.0 -21.611
Medium 
0         
 50 1035 474 0.458 9.48 26.0 0.000
Test Chemical tert-Butyl alcohol 
160         
 50 1037 459 0.443 9.18 26.0 -3.351 
500         
 50 1048 438 0.418 8.76 26.0 -8.741 
1600         
 50 1040 452 0.435 9.04 26.0 -5.100 
5000         
 50 1047 400 0.382 8.00 26.0 -16.579
Positive Control Cyclophosphamide 
 0.3       
 50 1041 606 0.582 12.12 26.0 27.111
Cyclophosphamide 
2         
 10 210 322 1.533 32.20 26.0 234.810
Trend: -2.495        
Probability: 0.994        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/08/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1038 469 0.452 9.38 26.0 0.000
Negative (Not Specified) 
0         
 50 1026 488 0.476 9.76 26.0 5.268
Test Chemical tert-Butyl alcohol 
2000         
 50 1047 505 0.482 10.10 26.0 6.750 
3000         
 50 1043 454 0.435 9.08 26.0 -3.662 
4000         
 50 1042 448 0.430 8.96 26.0 -4.844 
5000         
 50 1037 482 0.465 9.64 26.0 2.871
Positive Control Cyclophosphamide 
 0.3       
 50 1044 622 0.596 12.44 26.0 31.860
Cyclophosphamide 
2         
 10 210 319 1.519 31.90 26.0 236.199
Trend: -0.569        
Probability: 0.715