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Study Information
Study Attribute Value
CEBS Accession Number: 002-03206-0002-0000-5
Chemical Name: tert-Butyl alcohol
CASRN: 75-65-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 263726_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/03/1984    Harvest Time: 11 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Medium 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: tert-Butyl alcohol 
160         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
500         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
1600         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 42 0.420 32.0 11 0.110 10.0 29 0.290 24.0 2 0.020 2.0
Mitomycin-C 
1         
 50 27 0.540 28.0 8 0.160 16.0 8 0.160 14.0 11 0.220 2.0
Trend: -0.387 -1.225 0.209  
Probability: 0.651 0.890 0.417  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/02/1984    Harvest Time: 11.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: tert-Butyl alcohol 
160         
 100 4 0.040 4.0 1 0.010 1.0 2 0.020 2.0 1 0.010 1.0 
500         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
1600         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
5000         
 100 6 0.060 6.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 20 0.200 19.0 7 0.070 7.0 13 0.130 12.0 0 0.000 0.0
Cyclophosphamide 
50         
 100 61 0.610 42.0 36 0.360 29.0 24 0.240 20.0 1 0.010 1.0
Trend: 2.110 1.741 1.617  
Probability: 0.017 0.041 0.053  


Trial #:2   Activation: No Activation    Date: 03/23/1984    Harvest Time: 11.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: tert-Butyl alcohol 
1600         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
3000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
4000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 5 0.050 5.0 4 0.040 4.0 0 0.000 0.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.25      
 100 22 0.220 20.0 8 0.080 8.0 14 0.140 13.0 0 0.000 0.0
Mitomycin-C 
1         
 50 17 0.340 26.0 8 0.160 14.0 9 0.180 16.0 0 0.000 0.0
Trend: 0.358 0.940 -1.129  
Probability: 0.360 0.174 0.870  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/22/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: tert-Butyl alcohol 
1600         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
3000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
4000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
5000         
 13 1 0.077 8.0 0 0.000 0.0 1 0.077 8.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 23 0.230 19.0 12 0.120 10.0 11 0.110 10.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 61 1.220 70.0 24 0.480 36.0 37 0.740 50.0 0 0.000 0.0
Trend: 0.429 -0.342 0.829  
Probability: 0.334 0.634 0.204