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Study Information
Study Attribute Value
CEBS Accession Number: 002-03206-0023-0000-8
Chemical Name: tert-Butyl alcohol
CASRN: 75-65-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 097469
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
100# 90 94.5 32 33
    86 111 84 33
    86 94 100 39
    98 105 80 27
Test Chemical tert-Butyl alcohol
625         
125r 85 68 18
    99 131 67 23
 
1250         
116 102 130 38 38
    115 88 130 38
 
2500         
110 86 104 31
 
5000         
115 96 86 25 31
    95 74 108 38
Positive Control Methyl Methane Sulfonate
15         
46 27 352 255 227*
    52 36 309 199

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
54 93 157 98 92
    67 111 207 103
    60 96 160 90
    62 100 144 78
Test Chemical tert-Butyl alcohol
1000         
62 91 185 100 96
    55 112 153 93
 
2000         
41 87 160 130 127
    53 98 198 124
 
3000         
62 81 257 139 127
    55 90 191 115
 
4000         
51 78 243 158 142
    50 78 191 126
 
5000         
58 75 275 157 152*
    52 71 227 146
Positive Control Methyl Methane Sulfonate
15         
23 25 337 499 552*
    20 22 369 605

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
124r 110 160 43 49
    73 91 87 40
    80 108 135 56
    71 101 110 52
Test Chemical tert-Butyl alcohol
1000         
66 92 95 48 45
    95 106 122 43
 
2000         
92# 108 124 45 43
    77 101 96 42
 
3000         
82 99 110 45 41
    74 92 83 37
 
4000         
71 115 110 52
 
5000         
84 118 97 39 40
    81 102 100 41
Positive Control 3-Methylcholanthrene
2.5       
51 38 480 317 326*
    50 41 506 335

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
93 104 34 12 18
    103 105 67 22
    92 96 52 19
    70 95 37 18
Test Chemical tert-Butyl alcohol
2000         
80 94 44 18 26
    62 105 61 33
 
3000         
71 90 69 33 26
    78 90 45 19
 
4000         
80 90 47 20 17
    79 95 33 14
 
5000         
70 88 58 28 26
    97 101 69 24
Positive Control 3-Methylcholanthrene
2.5       
67 38 461 231 231*
    77 33 531 231

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination