Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03222-0010-0000-2
Chemical Name:	tris(2-Ethylhexyl)phosphate
CASRN:	78-42-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	094765
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	115	96	100	29	30
			113	87	106	31
			113	117	98	29
Test Chemical	tris(2-Ethylhexyl)phosphate	20	109	84	37	11	22
			110	63	74	22
			90	58	88	32
		30	114	66	71	21	23
			86	68	62	24
			110	68	78	24
		40	66	40	76	38	31
			111	57	104	31
			90	63	64	24
		50	88	74	76	29	28
			98	75	94	32
			108	71	78	24
		60	95	51	99	35	31
			109	53	86	26
			108	56	103	32
		80	108	63	67	21	25
			101	70	85	28
			109	73	81	25
Positive Control	Methyl Methane Sulfonate	5	54	20	612	380	377*
Positive Control			70	44	786	373	377*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	104	90	159	51	40
			109#	63	117	36
			116	158	108	31
			117	88	144	41
Test Chemical	tris(2-Ethylhexyl)phosphate	20	99	114	71	24	25
			93	89	52	19
			97	92	91	31
		30	91	78	97	36	31
			99	112	77	26
			100	88	94	31
		40	117	105	89	25	28
			100	79	95	32
			101	93	80	26
		50	107	101	96	30	31
			115	75	121	35
			118	79	95	27
		60	119	88	168	47	39
			104	28	117	38
			117	95	119	34
		80	113	27	98	29	35
			100	60	131	44
			118	100	118	33
Positive Control	Methyl Methane Sulfonate	5	33	12	676	686	610*
			43	22	906	700
			78	48	1040	443

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	111	119	140	42	49
			122r	140	116	32
			116	93	188	54
			100	88	157	52
Test Chemical	tris(2-Ethylhexyl)phosphate	20	97	107	116	40	37
			108	100	117	36
			115	111	125	36
		30	114	52	120	35	37
			85	64	91	36
			64	86	78	41
		40	112	91	113	34	32
			109	115	114	35
			105	108	85	27
		50	104	76	127	41	38
			107	103	135	42
			117	98	108	31
		60	107	95	124	39	41
			112	110	145	43
			111	86	139	42
		80	126r	121	125	33	33
			103#	85	95	31
			117	80	122	35
Positive Control	3-Methylcholanthrene	2.5	127r	59	788	207	165*
			100	46	494	165
			121r	48	756	209

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	114	59	220	65	48
			118	107	119	33
			115	116	150	43
			111	119	168	50
Test Chemical	tris(2-Ethylhexyl)phosphate	20	107	65	161	50	58
			79	59	139	59
			98	33	187	64
		30	104	19	205	66	56
			89	37	139	52
			114	55	176	51
		40	107	34	189	59	54
			107	41	182	57
			102	46	140	46
		50	105	33	188	60	58
			109	33	211	65
			101	27	152	50
		60	93	30	153	55	64
			106	34	219	69
			107	34	216	67
		80	111	36	144	43	53
			111	38	203	61
			104	29	167	54
Positive Control	3-Methylcholanthrene	2.5					348*
			42	3	355	281
			52	10	643	416

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination