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Study Information
Study Attribute Value
CEBS Accession Number: 002-03222-0010-0000-2
Chemical Name: tris(2-Ethylhexyl)phosphate
CASRN: 78-42-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 094765
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 96 100 29 30
    113 87 106 31
    113 117 98 29
Test Chemical tris(2-Ethylhexyl)phosphate 
20         
 109 84 37 11 22
    110 63 74 22
    90 58 88 32
  
30         
 114 66 71 21 23
    86 68 62 24
    110 68 78 24
  
40         
 66 40 76 38 31
    111 57 104 31
    90 63 64 24
  
50         
 88 74 76 29 28
    98 75 94 32
    108 71 78 24
  
60         
 95 51 99 35 31
    109 53 86 26
    108 56 103 32
  
80         
 108 63 67 21 25
    101 70 85 28
    109 73 81 25
Positive Control Methyl Methane Sulfonate 
5         
 54 20 612 380 377*
    70 44 786 373

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 104 90 159 51 40
    109# 63 117 36
    116 158 108 31
    117 88 144 41
Test Chemical tris(2-Ethylhexyl)phosphate 
20         
 99 114 71 24 25
    93 89 52 19
    97 92 91 31
  
30         
 91 78 97 36 31
    99 112 77 26
    100 88 94 31
  
40         
 117 105 89 25 28
    100 79 95 32
    101 93 80 26
  
50         
 107 101 96 30 31
    115 75 121 35
    118 79 95 27
  
60         
 119 88 168 47 39
    104 28 117 38
    117 95 119 34
  
80         
 113 27 98 29 35
    100 60 131 44
    118 100 118 33
Positive Control Methyl Methane Sulfonate 
5         
 33 12 676 686 610*
    43 22 906 700
    78 48 1040 443

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 111 119 140 42 49
    122r 140 116 32
    116 93 188 54
    100 88 157 52
Test Chemical tris(2-Ethylhexyl)phosphate 
20         
 97 107 116 40 37
    108 100 117 36
    115 111 125 36
  
30         
 114 52 120 35 37
    85 64 91 36
    64 86 78 41
  
40         
 112 91 113 34 32
    109 115 114 35
    105 108 85 27
  
50         
 104 76 127 41 38
    107 103 135 42
    117 98 108 31
  
60         
 107 95 124 39 41
    112 110 145 43
    111 86 139 42
  
80         
 126r 121 125 33 33
    103# 85 95 31
    117 80 122 35
Positive Control 3-Methylcholanthrene 
2.5       
 127r 59 788 207 165*
    100 46 494 165
    121r 48 756 209

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 114 59 220 65 48
    118 107 119 33
    115 116 150 43
    111 119 168 50
Test Chemical tris(2-Ethylhexyl)phosphate 
20         
 107 65 161 50 58
    79 59 139 59
    98 33 187 64
  
30         
 104 19 205 66 56
    89 37 139 52
    114 55 176 51
  
40         
 107 34 189 59 54
    107 41 182 57
    102 46 140 46
  
50         
 105 33 188 60 58
    109 33 211 65
    101 27 152 50
  
60         
 93 30 153 55 64
    106 34 219 69
    107 34 216 67
  
80         
 111 36 144 43 53
    111 38 203 61
    104 29 167 54
Positive Control 3-Methylcholanthrene 
2.5       
 348*
    42 3 355 281
    52 10 643 416

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination