Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03239-0001-0000-0
Chemical Name:	DL-menthol
CASRN:	89-78-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	979487
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	77	96	65	28	37
			96	94	134	47
			77	100	75	32
			78	110	95	41
Test Chemical	DL-menthol	12.5	68	91	83	41	36
			70	95	76	36
			72	99	68	31
		25	68	27	51	25	27
			83	108	83	33
			87	78	59	23
		50	74	71	74	33	38
			56	88	78	46
			69	56	73	35
		100	100	67	87	29	29
			74	65	62	28
			97	68	88	30
		150	68	25	59	29	33

			83	24	91	37
		200


Positive Control	Methyl Methane Sulfonate	5	40	34	480	400	488*
			33#	29	467	472
			37	16	659	591

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	61	82	45	24	27
			97#	105	80	27
			98	103	77	26
			94	110	86	30
Test Chemical	DL-menthol	25	73	69	73	33	30
			64	56	71	37
			76	84	44	19
		50	81	55	71	29	24
			83	73	43	17
			78	71	60	26
		75	102	54	64	21	19
			91	63	54	20
			82	28	39	16
		100	85	43	73	29	28
			81	34	73	30
			88	54	70	26
		150	91	21	95	35	29
			81	30	60	25
			89	29	76	29
Positive Control	Methyl Methane Sulfonate	5	65	38	602	310	274*
			67	43	523	260
			77	53	576	251

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	102	112	91	30	35
			81	108	88	36
			71	95	62	29
			63	84	88	47
Test Chemical	DL-menthol	25	73	92	74	34	42
			95	93	108	38
			58	65	93	53
		50	73	71	111	50	43
			71	76	88	41
			76	94	88	39
		75	76	68	109	48	45
			79	73	93	39
			72	80	102	47
		100	75	82	87	39	33
			89	81	93	35
			110	89	84	26
		150	75	71	78	35	33
			79	66	74	31
			107	144	104	32
		200					35
			95	52	122	43
			96	47	79	27
		300


Positive Control	3-Methylcholanthrene	2.5	105	71	390	124	145*
			84	63	315	125
			84	51	468	186

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	110	115	124	38	46
			122r	115	97	27
			84	96	136	54
			84	88	113	45
Test Chemical	DL-menthol	25	98	78	162	55	54
			100	62	159	53
			101	79	165	54
		50	109	56	174	53	58
			84	60	146	58
			104	66	197	63
		75	122r	65	194	53	53
			99	60	187	63
			83	52	106	43
		100	118	44	237	67	64
			104	70	160	51
			113	51	249	73
		150	115	46	125	36	37
			110	64	125	38
			111	46	126	38
		200


Positive Control	3-Methylcholanthrene	2.5	66	32	398	201	271*
			64	38	579	303
			73	33	676	310

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination