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Study Information
Study Attribute Value
CEBS Accession Number: 002-03239-0001-0000-0
Chemical Name: DL-menthol
CASRN: 89-78-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 979487
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 77 96 65 28 37
    96 94 134 47
    77 100 75 32
    78 110 95 41
Test Chemical DL-menthol 
12.5       
 68 91 83 41 36
    70 95 76 36
    72 99 68 31
  
25         
 68 27 51 25 27
    83 108 83 33
    87 78 59 23
  
50         
 74 71 74 33 38
    56 88 78 46
    69 56 73 35
  
100         
 100 67 87 29 29
    74 65 62 28
    97 68 88 30
  
150         
 68 25 59 29 33
   
    83 24 91 37
  
200         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 40 34 480 400 488*
    33# 29 467 472
    37 16 659 591

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 61 82 45 24 27
    97# 105 80 27
    98 103 77 26
    94 110 86 30
Test Chemical DL-menthol 
25         
 73 69 73 33 30
    64 56 71 37
    76 84 44 19
  
50         
 81 55 71 29 24
    83 73 43 17
    78 71 60 26
  
75         
 102 54 64 21 19
    91 63 54 20
    82 28 39 16
  
100         
 85 43 73 29 28
    81 34 73 30
    88 54 70 26
  
150         
 91 21 95 35 29
    81 30 60 25
    89 29 76 29
Positive Control Methyl Methane Sulfonate 
5         
 65 38 602 310 274*
    67 43 523 260
    77 53 576 251

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 102 112 91 30 35
    81 108 88 36
    71 95 62 29
    63 84 88 47
Test Chemical DL-menthol 
25         
 73 92 74 34 42
    95 93 108 38
    58 65 93 53
  
50         
 73 71 111 50 43
    71 76 88 41
    76 94 88 39
  
75         
 76 68 109 48 45
    79 73 93 39
    72 80 102 47
  
100         
 75 82 87 39 33
    89 81 93 35
    110 89 84 26
  
150         
 75 71 78 35 33
    79 66 74 31
    107 144 104 32
  
200         
 35
    95 52 122 43
    96 47 79 27
  
300         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 105 71 390 124 145*
    84 63 315 125
    84 51 468 186

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 110 115 124 38 46
    122r 115 97 27
    84 96 136 54
    84 88 113 45
Test Chemical DL-menthol 
25         
 98 78 162 55 54
    100 62 159 53
    101 79 165 54
  
50         
 109 56 174 53 58
    84 60 146 58
    104 66 197 63
  
75         
 122r 65 194 53 53
    99 60 187 63
    83 52 106 43
  
100         
 118 44 237 67 64
    104 70 160 51
    113 51 249 73
  
150         
 115 46 125 36 37
    110 64 125 38
    111 46 126 38
  
200         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 66 32 398 201 271*
    64 38 579 303
    73 33 676 310

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination