Background

… Regarding the data contained under “Please compare 400uM treatment group for each PFAS to 1% methanol 400uM group & please compare every other treatment group for each PFAS chemical to 1% methanol group”, could we also compare every other treatment group for each PFAS (in PDP groups) to the PFAS + 400uM (no PDP) group?

… Regarding the data contained under “Please compare 400uM treatment group for each PFAS to 1% methanol 400uM group & please compare every other treatment group for each PFAS chemical to 1% methanol group”, could we also compare every other treatment group for each PFAS (in PDP groups) to the PFAS + 400uM (no PDP) group?

Please compare 400uM treatment group for each PFAS to 1% methanol 400uM group & please compare every other treatment group for each PFAS chemical to 1% methanol group

Caov-3 PFAS BPD JC-1

Table 49 Exposure Group = 500nM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.4240558 0.0867033 18 4.890884 0.0003529
400µM - (PDP + 200µM) 0.3094259 0.0867033 18 3.568790 0.0065686
400µM - (PDP + 400µM) 0.4039052 0.0867033 18 4.658475 0.0005863
Table 49 Exposure Group = 2µM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1761703 0.0907683 18 1.940880 0.1906983
400µM - (PDP + 200µM) 0.0939821 0.0907683 18 1.035407 0.6774390
400µM - (PDP + 400µM) 0.1234923 0.0907683 18 1.360523 0.4694620
Table 49 Exposure Group = 500nM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2036904 0.1824512 18 1.1164103 0.6250832
400µM - (PDP + 200µM) 0.1150767 0.1824512 18 0.6307260 0.9001973
400µM - (PDP + 400µM) 0.1804363 0.1824512 18 0.9889562 0.7069713
Table 49 Exposure Group = 2µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.4341654 0.0905067 18 4.797052 0.0004329
400µM - (PDP + 200µM) 0.3148018 0.0905067 18 3.478214 0.0080286
400µM - (PDP + 400µM) 0.2949365 0.0905067 18 3.258725 0.0130253
Table 49 Exposure Group = 500nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2497773 0.0855414 18 2.919958 0.0271772
400µM - (PDP + 200µM) 0.2987212 0.0855414 18 3.492124 0.0077852
400µM - (PDP + 400µM) 0.3155662 0.0855414 18 3.689045 0.0050289
Table 49 Exposure Group = 2µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2121959 0.0559556 18 3.792217 0.0039977
400µM - (PDP + 200µM) 0.2528818 0.0559556 18 4.519328 0.0007961
400µM - (PDP + 400µM) 0.2467282 0.0559556 18 4.409354 0.0010148

[1] 50

Caov-3 PFAS ALA JC-1

Table 50 Exposure Group = 500nM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0997520 0.0433139 18 2.303002 0.0969590
400µM - (PDP + 200µM) 0.0834565 0.0433139 18 1.926785 0.1954976
400µM - (PDP + 400µM) 0.0610038 0.0433139 18 1.408413 0.4406210
Table 50 Exposure Group = 2µM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0970916 0.1148713 18 0.8452203 0.7936617
400µM - (PDP + 200µM) 0.0781766 0.1148713 18 0.6805585 0.8785741
400µM - (PDP + 400µM) -0.0513803 0.1148713 18 -0.4472858 0.9606976
Table 50 Exposure Group = 500nM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2182626 0.098479 18 2.2163366 0.1146836
400µM - (PDP + 200µM) 0.1114753 0.098479 18 1.1319699 0.6149698
400µM - (PDP + 400µM) 0.0438115 0.098479 18 0.4448815 0.9612856
Table 50 Exposure Group = 2µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1676686 0.1049636 18 1.597398 0.3359941
400µM - (PDP + 200µM) 0.1546944 0.1049636 18 1.473791 0.4026521
400µM - (PDP + 400µM) 0.1088639 0.1049636 18 1.037158 0.6763167
Table 50 Exposure Group = 500nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2564289 0.0634723 18 4.040015 0.0023029
400µM - (PDP + 200µM) 0.2760167 0.0634723 18 4.348619 0.0011608
400µM - (PDP + 400µM) 0.3234912 0.0634723 18 5.096576 0.0002261
Table 50 Exposure Group = 2µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1241074 0.0843376 18 1.4715537 0.4039222
400µM - (PDP + 200µM) 0.1151627 0.0843376 18 1.3654963 0.4664302
400µM - (PDP + 400µM) 0.0582280 0.0843376 18 0.6904154 0.8740524

[1] 51

Caov-3 Mix BPD JC-1

Table 51 Exposure Group = 1µM PFOA + 1µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0603742 0.0312739 18 1.9305012 0.1942230
400µM - (PDP + 200µM) 0.0209217 0.0312739 18 0.6689833 0.8837827
400µM - (PDP + 400µM) -0.0279003 0.0312739 18 -0.8921281 0.7663640
Table 51 Exposure Group = 1µM PFOA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.3117711 0.0787618 18 3.958405 0.0027615
400µM - (PDP + 200µM) 0.3070296 0.0787618 18 3.898205 0.0031575
400µM - (PDP + 400µM) 0.2050476 0.0787618 18 2.603389 0.0529495
Table 51 Exposure Group = 1µM PFHpA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2084158 0.0485454 18 4.293216 0.0013123
400µM - (PDP + 200µM) 0.2095300 0.0485454 18 4.316168 0.0012472
400µM - (PDP + 400µM) 0.1112362 0.0485454 18 2.291386 0.0991847
Table 51 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2530215 0.0495669 18 5.104650 0.0002222
400µM - (PDP + 200µM) 0.3332640 0.0495669 18 6.723523 0.0000080
400µM - (PDP + 400µM) 0.1733317 0.0495669 18 3.496927 0.0077028

Caov-3 Mix ALA JC-1

Table 52 Exposure Group = 1µM PFOA + 1µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0640151 0.0327962 18 1.9519033 0.1870112
400µM - (PDP + 200µM) -0.0028912 0.0327962 18 -0.0881564 0.9996676
400µM - (PDP + 400µM) -0.0001791 0.0327962 18 -0.0054597 0.9999999
Table 52 Exposure Group = 1µM PFOA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1608782 0.0542917 18 2.963220 0.0247664
400µM - (PDP + 200µM) 0.1484390 0.0542917 18 2.734102 0.0403249
400µM - (PDP + 400µM) 0.0855843 0.0542917 18 1.576381 0.3468196
Table 52 Exposure Group = 1µM PFHpA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.3094544 0.0627626 18 4.930555 0.0003237
400µM - (PDP + 200µM) 0.2637276 0.0627626 18 4.201988 0.0016067
400µM - (PDP + 400µM) 0.2497817 0.0627626 18 3.979786 0.0026332
Table 52 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2333357 0.0397375 18 5.871924 4.40e-05
400µM - (PDP + 200µM) 0.2717649 0.0397375 18 6.839001 6.40e-06
400µM - (PDP + 400µM) 0.2385095 0.0397375 18 6.002123 3.37e-05

OVCAR-3 PFAS BPD JC-1

Table 53 Exposure Group = 500nM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0349018 0.0453986 18 0.7687858 0.8354248
400µM - (PDP + 200µM) 0.0885079 0.0453986 18 1.9495712 0.1877865
400µM - (PDP + 400µM) 0.0125019 0.0453986 18 0.2753798 0.9902217
Table 53 Exposure Group = 2µM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2444571 0.0505905 18 4.832079 0.0004011
400µM - (PDP + 200µM) 0.2425476 0.0505905 18 4.794335 0.0004355
400µM - (PDP + 400µM) 0.2368486 0.0505905 18 4.681686 0.0005572
Table 53 Exposure Group = 500nM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.4062657 0.0512441 18 7.928044 8.00e-07
400µM - (PDP + 200µM) 0.3875144 0.0512441 18 7.562123 1.60e-06
400µM - (PDP + 400µM) 0.3033588 0.0512441 18 5.919874 3.99e-05
Table 53 Exposure Group = 2µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.6238421 0.074582 18 8.364514 4e-07
400µM - (PDP + 200µM) 0.6253209 0.074582 18 8.384343 4e-07
400µM - (PDP + 400µM) 0.6120187 0.074582 18 8.205985 5e-07
Table 53 Exposure Group = 500nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1524101 0.0502788 18 3.031298 0.0213835
400µM - (PDP + 200µM) 0.1421489 0.0502788 18 2.827212 0.0331239
400µM - (PDP + 400µM) 0.0527670 0.0502788 18 1.049488 0.6683975
Table 53 Exposure Group = 2µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.3214438 0.0755952 18 4.252170 0.0014374
400µM - (PDP + 200µM) 0.3678116 0.0755952 18 4.865540 0.0003729
400µM - (PDP + 400µM) 0.4053611 0.0755952 18 5.362257 0.0001280

OVCAR-3 PFAS ALA JC-1

Table 54 Exposure Group = 500nM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) -0.0049281 0.1150399 18 -0.0428383 0.9999617
400µM - (PDP + 200µM) -0.1534356 0.1150399 18 -1.3337598 0.4859137
400µM - (PDP + 400µM) -0.3452395 0.1150399 18 -3.0010406 0.0228282
Table 54 Exposure Group = 2µM PFOA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1894212 0.0474101 18 3.995379 0.0025434
400µM - (PDP + 200µM) 0.1923231 0.0474101 18 4.056588 0.0022196
400µM - (PDP + 400µM) 0.0756666 0.0474101 18 1.596002 0.3367063
Table 54 Exposure Group = 500nM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2452792 0.0793555 18 3.090890 0.0187920
400µM - (PDP + 200µM) 0.1277620 0.0793555 18 1.609995 0.3296091
400µM - (PDP + 400µM) 0.2466521 0.0793555 18 3.108191 0.0180984
Table 54 Exposure Group = 2µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2765781 0.0957513 18 2.888506 0.0290694
400µM - (PDP + 200µM) 0.2970665 0.0957513 18 3.102482 0.0183245
400µM - (PDP + 400µM) 0.2246273 0.0957513 18 2.345947 0.0891112
Table 54 Exposure Group = 500nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1078832 0.07604 18 1.418768 0.4344938
400µM - (PDP + 200µM) 0.1581810 0.07604 18 2.080232 0.1482030
400µM - (PDP + 400µM) 0.1272306 0.07604 18 1.673205 0.2987710
Table 54 Exposure Group = 2µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.3557679 0.0730301 18 4.871526 0.0003680
400µM - (PDP + 200µM) 0.3190538 0.0730301 18 4.368800 0.0011101
400µM - (PDP + 400µM) 0.2037932 0.0730301 18 2.790538 0.0358012

OVCAR-3 Mix BPD JC-1

Table 55 Exposure Group = 1µM PFOA + 1µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.1952629 0.0545781 18 3.577679 0.0064403
400µM - (PDP + 200µM) 0.1799426 0.0545781 18 3.296974 0.0119758
400µM - (PDP + 400µM) 0.1133262 0.0545781 18 2.076405 0.1492548
Table 55 Exposure Group = 1µM PFOA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.4073707 0.0450896 18 9.034697 1.0e-07
400µM - (PDP + 200µM) 0.3896068 0.0450896 18 8.640729 2.0e-07
400µM - (PDP + 400µM) 0.3187198 0.0450896 18 7.068591 4.1e-06
Table 55 Exposure Group = 1µM PFHpA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.5818892 0.0455659 18 12.77029 0
400µM - (PDP + 200µM) 0.5825011 0.0455659 18 12.78372 0
400µM - (PDP + 400µM) 0.5012934 0.0455659 18 11.00151 0
Table 55 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.8660313 0.0729195 18 11.87654 0
400µM - (PDP + 200µM) 0.8716574 0.0729195 18 11.95370 0
400µM - (PDP + 400µM) 0.8352314 0.0729195 18 11.45416 0

OVCAR-3 Mix ALA JC-1

Table 56 Exposure Group = 1µM PFOA + 1µM PFHpA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0466285 0.0758841 18 0.6144696 0.9067904
400µM - (PDP + 200µM) 0.0580172 0.0758841 18 0.7645504 0.8376277
400µM - (PDP + 400µM) -0.1672128 0.0758841 18 -2.2035287 0.1175304
Table 56 Exposure Group = 1µM PFOA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0005325 0.0837344 18 0.0063590 0.9999999
400µM - (PDP + 200µM) 0.0050649 0.0837344 18 0.0604881 0.9998924
400µM - (PDP + 400µM) 0.0295840 0.0837344 18 0.3533081 0.9798678
Table 56 Exposure Group = 1µM PFHpA + 1µM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.2588241 0.092625 18 2.794321 0.0355159
400µM - (PDP + 200µM) 0.2770016 0.092625 18 2.990569 0.0233497
400µM - (PDP + 400µM) 0.3107560 0.092625 18 3.354990 0.0105402
Table 56 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
contrast estimate SE df t.ratio p.value
400µM - (PDP + 100µM) 0.0518385 0.1490019 18 0.3479051 0.9807397
400µM - (PDP + 200µM) 0.2427964 0.1490019 18 1.6294851 0.3198863
400µM - (PDP + 400µM) 0.2987034 0.1490019 18 2.0046945 0.1701439