Background
… Regarding the data contained under “Please compare 400uM treatment
group for each PFAS to 1% methanol 400uM group & please compare
every other treatment group for each PFAS chemical to 1% methanol
group”, could we also compare every other treatment group for each PFAS
(in PDP groups) to the PFAS + 400uM (no PDP) group?
… Regarding the data contained under “Please compare 400uM treatment
group for each PFAS to 1% methanol 400uM group & please compare
every other treatment group for each PFAS chemical to 1% methanol
group”, could we also compare every other treatment group for each PFAS
(in PDP groups) to the PFAS + 400uM (no PDP) group?
Please compare 400uM treatment group for each PFAS to 1% methanol
400uM group & please compare every other treatment group for each
PFAS chemical to 1% methanol group
Caov-3 PFAS BPD JC-1
Table 49 Exposure Group = 500nM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.4240558
|
0.0867033
|
18
|
4.890884
|
0.0003529
|
|
400µM - (PDP + 200µM)
|
0.3094259
|
0.0867033
|
18
|
3.568790
|
0.0065686
|
|
400µM - (PDP + 400µM)
|
0.4039052
|
0.0867033
|
18
|
4.658475
|
0.0005863
|
Table 49 Exposure Group = 2µM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1761703
|
0.0907683
|
18
|
1.940880
|
0.1906983
|
|
400µM - (PDP + 200µM)
|
0.0939821
|
0.0907683
|
18
|
1.035407
|
0.6774390
|
|
400µM - (PDP + 400µM)
|
0.1234923
|
0.0907683
|
18
|
1.360523
|
0.4694620
|
Table 49 Exposure Group = 500nM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2036904
|
0.1824512
|
18
|
1.1164103
|
0.6250832
|
|
400µM - (PDP + 200µM)
|
0.1150767
|
0.1824512
|
18
|
0.6307260
|
0.9001973
|
|
400µM - (PDP + 400µM)
|
0.1804363
|
0.1824512
|
18
|
0.9889562
|
0.7069713
|
Table 49 Exposure Group = 2µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.4341654
|
0.0905067
|
18
|
4.797052
|
0.0004329
|
|
400µM - (PDP + 200µM)
|
0.3148018
|
0.0905067
|
18
|
3.478214
|
0.0080286
|
|
400µM - (PDP + 400µM)
|
0.2949365
|
0.0905067
|
18
|
3.258725
|
0.0130253
|
Table 49 Exposure Group = 500nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2497773
|
0.0855414
|
18
|
2.919958
|
0.0271772
|
|
400µM - (PDP + 200µM)
|
0.2987212
|
0.0855414
|
18
|
3.492124
|
0.0077852
|
|
400µM - (PDP + 400µM)
|
0.3155662
|
0.0855414
|
18
|
3.689045
|
0.0050289
|
Table 49 Exposure Group = 2µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2121959
|
0.0559556
|
18
|
3.792217
|
0.0039977
|
|
400µM - (PDP + 200µM)
|
0.2528818
|
0.0559556
|
18
|
4.519328
|
0.0007961
|
|
400µM - (PDP + 400µM)
|
0.2467282
|
0.0559556
|
18
|
4.409354
|
0.0010148
|
[1] 50
Caov-3 PFAS ALA JC-1
Table 50 Exposure Group = 500nM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0997520
|
0.0433139
|
18
|
2.303002
|
0.0969590
|
|
400µM - (PDP + 200µM)
|
0.0834565
|
0.0433139
|
18
|
1.926785
|
0.1954976
|
|
400µM - (PDP + 400µM)
|
0.0610038
|
0.0433139
|
18
|
1.408413
|
0.4406210
|
Table 50 Exposure Group = 2µM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0970916
|
0.1148713
|
18
|
0.8452203
|
0.7936617
|
|
400µM - (PDP + 200µM)
|
0.0781766
|
0.1148713
|
18
|
0.6805585
|
0.8785741
|
|
400µM - (PDP + 400µM)
|
-0.0513803
|
0.1148713
|
18
|
-0.4472858
|
0.9606976
|
Table 50 Exposure Group = 500nM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2182626
|
0.098479
|
18
|
2.2163366
|
0.1146836
|
|
400µM - (PDP + 200µM)
|
0.1114753
|
0.098479
|
18
|
1.1319699
|
0.6149698
|
|
400µM - (PDP + 400µM)
|
0.0438115
|
0.098479
|
18
|
0.4448815
|
0.9612856
|
Table 50 Exposure Group = 2µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1676686
|
0.1049636
|
18
|
1.597398
|
0.3359941
|
|
400µM - (PDP + 200µM)
|
0.1546944
|
0.1049636
|
18
|
1.473791
|
0.4026521
|
|
400µM - (PDP + 400µM)
|
0.1088639
|
0.1049636
|
18
|
1.037158
|
0.6763167
|
Table 50 Exposure Group = 500nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2564289
|
0.0634723
|
18
|
4.040015
|
0.0023029
|
|
400µM - (PDP + 200µM)
|
0.2760167
|
0.0634723
|
18
|
4.348619
|
0.0011608
|
|
400µM - (PDP + 400µM)
|
0.3234912
|
0.0634723
|
18
|
5.096576
|
0.0002261
|
Table 50 Exposure Group = 2µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1241074
|
0.0843376
|
18
|
1.4715537
|
0.4039222
|
|
400µM - (PDP + 200µM)
|
0.1151627
|
0.0843376
|
18
|
1.3654963
|
0.4664302
|
|
400µM - (PDP + 400µM)
|
0.0582280
|
0.0843376
|
18
|
0.6904154
|
0.8740524
|
[1] 51
Caov-3 Mix BPD JC-1
Table 51 Exposure Group = 1µM PFOA + 1µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0603742
|
0.0312739
|
18
|
1.9305012
|
0.1942230
|
|
400µM - (PDP + 200µM)
|
0.0209217
|
0.0312739
|
18
|
0.6689833
|
0.8837827
|
|
400µM - (PDP + 400µM)
|
-0.0279003
|
0.0312739
|
18
|
-0.8921281
|
0.7663640
|
Table 51 Exposure Group = 1µM PFOA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.3117711
|
0.0787618
|
18
|
3.958405
|
0.0027615
|
|
400µM - (PDP + 200µM)
|
0.3070296
|
0.0787618
|
18
|
3.898205
|
0.0031575
|
|
400µM - (PDP + 400µM)
|
0.2050476
|
0.0787618
|
18
|
2.603389
|
0.0529495
|
Table 51 Exposure Group = 1µM PFHpA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2084158
|
0.0485454
|
18
|
4.293216
|
0.0013123
|
|
400µM - (PDP + 200µM)
|
0.2095300
|
0.0485454
|
18
|
4.316168
|
0.0012472
|
|
400µM - (PDP + 400µM)
|
0.1112362
|
0.0485454
|
18
|
2.291386
|
0.0991847
|
Table 51 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2530215
|
0.0495669
|
18
|
5.104650
|
0.0002222
|
|
400µM - (PDP + 200µM)
|
0.3332640
|
0.0495669
|
18
|
6.723523
|
0.0000080
|
|
400µM - (PDP + 400µM)
|
0.1733317
|
0.0495669
|
18
|
3.496927
|
0.0077028
|
Caov-3 Mix ALA JC-1
Table 52 Exposure Group = 1µM PFOA + 1µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0640151
|
0.0327962
|
18
|
1.9519033
|
0.1870112
|
|
400µM - (PDP + 200µM)
|
-0.0028912
|
0.0327962
|
18
|
-0.0881564
|
0.9996676
|
|
400µM - (PDP + 400µM)
|
-0.0001791
|
0.0327962
|
18
|
-0.0054597
|
0.9999999
|
Table 52 Exposure Group = 1µM PFOA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1608782
|
0.0542917
|
18
|
2.963220
|
0.0247664
|
|
400µM - (PDP + 200µM)
|
0.1484390
|
0.0542917
|
18
|
2.734102
|
0.0403249
|
|
400µM - (PDP + 400µM)
|
0.0855843
|
0.0542917
|
18
|
1.576381
|
0.3468196
|
Table 52 Exposure Group = 1µM PFHpA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.3094544
|
0.0627626
|
18
|
4.930555
|
0.0003237
|
|
400µM - (PDP + 200µM)
|
0.2637276
|
0.0627626
|
18
|
4.201988
|
0.0016067
|
|
400µM - (PDP + 400µM)
|
0.2497817
|
0.0627626
|
18
|
3.979786
|
0.0026332
|
Table 52 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2333357
|
0.0397375
|
18
|
5.871924
|
4.40e-05
|
|
400µM - (PDP + 200µM)
|
0.2717649
|
0.0397375
|
18
|
6.839001
|
6.40e-06
|
|
400µM - (PDP + 400µM)
|
0.2385095
|
0.0397375
|
18
|
6.002123
|
3.37e-05
|
OVCAR-3 PFAS BPD JC-1
Table 53 Exposure Group = 500nM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0349018
|
0.0453986
|
18
|
0.7687858
|
0.8354248
|
|
400µM - (PDP + 200µM)
|
0.0885079
|
0.0453986
|
18
|
1.9495712
|
0.1877865
|
|
400µM - (PDP + 400µM)
|
0.0125019
|
0.0453986
|
18
|
0.2753798
|
0.9902217
|
Table 53 Exposure Group = 2µM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2444571
|
0.0505905
|
18
|
4.832079
|
0.0004011
|
|
400µM - (PDP + 200µM)
|
0.2425476
|
0.0505905
|
18
|
4.794335
|
0.0004355
|
|
400µM - (PDP + 400µM)
|
0.2368486
|
0.0505905
|
18
|
4.681686
|
0.0005572
|
Table 53 Exposure Group = 500nM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.4062657
|
0.0512441
|
18
|
7.928044
|
8.00e-07
|
|
400µM - (PDP + 200µM)
|
0.3875144
|
0.0512441
|
18
|
7.562123
|
1.60e-06
|
|
400µM - (PDP + 400µM)
|
0.3033588
|
0.0512441
|
18
|
5.919874
|
3.99e-05
|
Table 53 Exposure Group = 2µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.6238421
|
0.074582
|
18
|
8.364514
|
4e-07
|
|
400µM - (PDP + 200µM)
|
0.6253209
|
0.074582
|
18
|
8.384343
|
4e-07
|
|
400µM - (PDP + 400µM)
|
0.6120187
|
0.074582
|
18
|
8.205985
|
5e-07
|
Table 53 Exposure Group = 500nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1524101
|
0.0502788
|
18
|
3.031298
|
0.0213835
|
|
400µM - (PDP + 200µM)
|
0.1421489
|
0.0502788
|
18
|
2.827212
|
0.0331239
|
|
400µM - (PDP + 400µM)
|
0.0527670
|
0.0502788
|
18
|
1.049488
|
0.6683975
|
Table 53 Exposure Group = 2µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.3214438
|
0.0755952
|
18
|
4.252170
|
0.0014374
|
|
400µM - (PDP + 200µM)
|
0.3678116
|
0.0755952
|
18
|
4.865540
|
0.0003729
|
|
400µM - (PDP + 400µM)
|
0.4053611
|
0.0755952
|
18
|
5.362257
|
0.0001280
|
OVCAR-3 PFAS ALA JC-1
Table 54 Exposure Group = 500nM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
-0.0049281
|
0.1150399
|
18
|
-0.0428383
|
0.9999617
|
|
400µM - (PDP + 200µM)
|
-0.1534356
|
0.1150399
|
18
|
-1.3337598
|
0.4859137
|
|
400µM - (PDP + 400µM)
|
-0.3452395
|
0.1150399
|
18
|
-3.0010406
|
0.0228282
|
Table 54 Exposure Group = 2µM PFOA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1894212
|
0.0474101
|
18
|
3.995379
|
0.0025434
|
|
400µM - (PDP + 200µM)
|
0.1923231
|
0.0474101
|
18
|
4.056588
|
0.0022196
|
|
400µM - (PDP + 400µM)
|
0.0756666
|
0.0474101
|
18
|
1.596002
|
0.3367063
|
Table 54 Exposure Group = 500nM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2452792
|
0.0793555
|
18
|
3.090890
|
0.0187920
|
|
400µM - (PDP + 200µM)
|
0.1277620
|
0.0793555
|
18
|
1.609995
|
0.3296091
|
|
400µM - (PDP + 400µM)
|
0.2466521
|
0.0793555
|
18
|
3.108191
|
0.0180984
|
Table 54 Exposure Group = 2µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2765781
|
0.0957513
|
18
|
2.888506
|
0.0290694
|
|
400µM - (PDP + 200µM)
|
0.2970665
|
0.0957513
|
18
|
3.102482
|
0.0183245
|
|
400µM - (PDP + 400µM)
|
0.2246273
|
0.0957513
|
18
|
2.345947
|
0.0891112
|
Table 54 Exposure Group = 500nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1078832
|
0.07604
|
18
|
1.418768
|
0.4344938
|
|
400µM - (PDP + 200µM)
|
0.1581810
|
0.07604
|
18
|
2.080232
|
0.1482030
|
|
400µM - (PDP + 400µM)
|
0.1272306
|
0.07604
|
18
|
1.673205
|
0.2987710
|
Table 54 Exposure Group = 2µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.3557679
|
0.0730301
|
18
|
4.871526
|
0.0003680
|
|
400µM - (PDP + 200µM)
|
0.3190538
|
0.0730301
|
18
|
4.368800
|
0.0011101
|
|
400µM - (PDP + 400µM)
|
0.2037932
|
0.0730301
|
18
|
2.790538
|
0.0358012
|
OVCAR-3 Mix BPD JC-1
Table 55 Exposure Group = 1µM PFOA + 1µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.1952629
|
0.0545781
|
18
|
3.577679
|
0.0064403
|
|
400µM - (PDP + 200µM)
|
0.1799426
|
0.0545781
|
18
|
3.296974
|
0.0119758
|
|
400µM - (PDP + 400µM)
|
0.1133262
|
0.0545781
|
18
|
2.076405
|
0.1492548
|
Table 55 Exposure Group = 1µM PFOA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.4073707
|
0.0450896
|
18
|
9.034697
|
1.0e-07
|
|
400µM - (PDP + 200µM)
|
0.3896068
|
0.0450896
|
18
|
8.640729
|
2.0e-07
|
|
400µM - (PDP + 400µM)
|
0.3187198
|
0.0450896
|
18
|
7.068591
|
4.1e-06
|
Table 55 Exposure Group = 1µM PFHpA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.5818892
|
0.0455659
|
18
|
12.77029
|
0
|
|
400µM - (PDP + 200µM)
|
0.5825011
|
0.0455659
|
18
|
12.78372
|
0
|
|
400µM - (PDP + 400µM)
|
0.5012934
|
0.0455659
|
18
|
11.00151
|
0
|
Table 55 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.8660313
|
0.0729195
|
18
|
11.87654
|
0
|
|
400µM - (PDP + 200µM)
|
0.8716574
|
0.0729195
|
18
|
11.95370
|
0
|
|
400µM - (PDP + 400µM)
|
0.8352314
|
0.0729195
|
18
|
11.45416
|
0
|
OVCAR-3 Mix ALA JC-1
Table 56 Exposure Group = 1µM PFOA + 1µM PFHpA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0466285
|
0.0758841
|
18
|
0.6144696
|
0.9067904
|
|
400µM - (PDP + 200µM)
|
0.0580172
|
0.0758841
|
18
|
0.7645504
|
0.8376277
|
|
400µM - (PDP + 400µM)
|
-0.1672128
|
0.0758841
|
18
|
-2.2035287
|
0.1175304
|
Table 56 Exposure Group = 1µM PFOA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0005325
|
0.0837344
|
18
|
0.0063590
|
0.9999999
|
|
400µM - (PDP + 200µM)
|
0.0050649
|
0.0837344
|
18
|
0.0604881
|
0.9998924
|
|
400µM - (PDP + 400µM)
|
0.0295840
|
0.0837344
|
18
|
0.3533081
|
0.9798678
|
Table 56 Exposure Group = 1µM PFHpA + 1µM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.2588241
|
0.092625
|
18
|
2.794321
|
0.0355159
|
|
400µM - (PDP + 200µM)
|
0.2770016
|
0.092625
|
18
|
2.990569
|
0.0233497
|
|
400µM - (PDP + 400µM)
|
0.3107560
|
0.092625
|
18
|
3.354990
|
0.0105402
|
Table 56 Exposure Group = 750nM PFOA + 750nM PFHpA + 750nM PFPA
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
400µM - (PDP + 100µM)
|
0.0518385
|
0.1490019
|
18
|
0.3479051
|
0.9807397
|
|
400µM - (PDP + 200µM)
|
0.2427964
|
0.1490019
|
18
|
1.6294851
|
0.3198863
|
|
400µM - (PDP + 400µM)
|
0.2987034
|
0.1490019
|
18
|
2.0046945
|
0.1701439
|