Background
Attached are data files for your analysis. I have specified in each
sheet what the requested comparisons are. They are very similar to those
that we’ve sent you previously for our first paper together. I totally
understand if you can’t get through all data before the holidays, but if
you could prioritize the HEC-1 data file, that would be great. Please
feel free to reach out if you have any questions!
HEC-1 MeOH
Sheet 1: Please compare all groups to zero. Sheet 2-9: Please compare
all groups to NT.
Sheet 10: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
HEC-1 MeOH
Table 1
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
0 - 0.1%
|
-0.0240355
|
0.0256163
|
168
|
-0.9382919
|
0.9791273
|
|
0 - 0.25%
|
0.0057978
|
0.0256163
|
168
|
0.2263329
|
0.9999998
|
|
0 - 0.5%
|
0.0174395
|
0.0256163
|
168
|
0.6807990
|
0.9979365
|
|
0 - 0.75%
|
0.0178881
|
0.0256163
|
168
|
0.6983104
|
0.9974935
|
|
0 - 1%
|
0.0169727
|
0.0256163
|
168
|
0.6625758
|
0.9983265
|
|
0 - 2%
|
0.0941674
|
0.0256163
|
168
|
3.6760777
|
0.0028616
|
|
0 - 3%
|
0.2227383
|
0.0256163
|
168
|
8.6951864
|
0.0000000
|
|
0 - 4%
|
0.7655813
|
0.0256163
|
168
|
29.8865170
|
0.0000000
|
|
0 - 5%
|
0.9922888
|
0.0256163
|
168
|
38.7366532
|
0.0000000
|
[1] 2
HEC-1 PFOA
Table 2
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
100nM - NT
|
0.0443752
|
0.0268993
|
51
|
1.6496792
|
0.6321003
|
|
1μM - NT
|
0.0881378
|
0.0268993
|
51
|
3.2765818
|
0.0169169
|
|
250nM - NT
|
0.0129172
|
0.0268993
|
51
|
0.4802060
|
0.9998796
|
|
25nM - NT
|
-0.0026667
|
0.0268993
|
51
|
-0.0991347
|
1.0000000
|
|
2μM - NT
|
0.0673957
|
0.0268993
|
51
|
2.5054790
|
0.1308626
|
|
500nM - NT
|
0.0440564
|
0.0268993
|
51
|
1.6378256
|
0.6410996
|
|
50nM - NT
|
0.0072782
|
0.0268993
|
51
|
0.2705727
|
0.9999991
|
|
750nM - NT
|
-0.0185703
|
0.0268993
|
51
|
-0.6903642
|
0.9977903
|
|
75nM - NT
|
0.0273853
|
0.0268993
|
51
|
1.0180662
|
0.9661126
|
[1] 3
HEC-1 PFHpA
Table 3
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
100nM - NT
|
0.0474075
|
0.0284919
|
51
|
1.6638926
|
0.6212760
|
|
1μM - NT
|
0.0119397
|
0.0284919
|
51
|
0.4190569
|
0.9999617
|
|
250nM - NT
|
0.0523877
|
0.0284919
|
51
|
1.8386867
|
0.4885220
|
|
25nM - NT
|
0.0578581
|
0.0284919
|
51
|
2.0306824
|
0.3547668
|
|
2μM - NT
|
0.1031658
|
0.0284919
|
51
|
3.6208758
|
0.0060599
|
|
500nM - NT
|
0.0811441
|
0.0284919
|
51
|
2.8479689
|
0.0555276
|
|
50nM - NT
|
0.0840356
|
0.0284919
|
51
|
2.9494531
|
0.0423433
|
|
750nM - NT
|
0.0476043
|
0.0284919
|
51
|
1.6707982
|
0.6160063
|
|
75nM - NT
|
0.0707618
|
0.0284919
|
51
|
2.4835724
|
0.1377656
|
[1] 4
HEC-1 PFPA
Table 4
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
100nM - NT
|
-0.0078103
|
0.0287836
|
51
|
-0.2713464
|
0.9999991
|
|
1μM - NT
|
0.0941768
|
0.0287836
|
51
|
3.2718914
|
0.0171483
|
|
250nM - NT
|
0.0671793
|
0.0287836
|
51
|
2.3339434
|
0.1932422
|
|
25nM - NT
|
0.0598736
|
0.0287836
|
51
|
2.0801301
|
0.3238917
|
|
2μM - NT
|
0.0191803
|
0.0287836
|
51
|
0.6663623
|
0.9983164
|
|
500nM - NT
|
0.0883111
|
0.0287836
|
51
|
3.0681043
|
0.0305801
|
|
50nM - NT
|
0.0888516
|
0.0287836
|
51
|
3.0868851
|
0.0290215
|
|
750nM - NT
|
0.0809460
|
0.0287836
|
51
|
2.8122266
|
0.0609859
|
|
75nM - NT
|
0.0632863
|
0.0287836
|
51
|
2.1986944
|
0.2569579
|
[1] 5
HEC-1 PFOA + PFHpA
Table 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
(1uM + 1uM) - NT
|
0.0270827
|
0.0234019
|
44
|
1.1572859
|
0.6892918
|
|
(250nM + 250nM) - NT
|
0.0145707
|
0.0234019
|
44
|
0.6226280
|
0.9539437
|
|
(2uM + 2uM) - NT
|
-0.0336901
|
0.0234019
|
44
|
-1.4396295
|
0.4950964
|
|
(500nM + 500nM) - NT
|
-0.0103247
|
0.0234019
|
44
|
-0.4411918
|
0.9868297
|
[1] 6
HEC-1 PFOA + PFPA
Table 6
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
(1uM + 1uM) - NT
|
-0.0712046
|
0.0243265
|
44
|
-2.9270414
|
0.0214193
|
|
(250nM + 250nM) - NT
|
-0.0111460
|
0.0243265
|
44
|
-0.4581836
|
0.9848346
|
|
(2uM + 2uM) - NT
|
-0.0190775
|
0.0243265
|
44
|
-0.7842285
|
0.8996076
|
|
(500nM + 500nM) - NT
|
0.0105355
|
0.0243265
|
44
|
0.4330868
|
0.9877136
|
[1] 7
HEC-1 PFHpA + PFPA
Table 7
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
(1uM + 1uM) - NT
|
-0.0155967
|
0.0218523
|
44
|
-0.7137317
|
0.9264114
|
|
(250nM + 250nM) - NT
|
0.0029442
|
0.0218523
|
44
|
0.1347305
|
0.9998711
|
|
(2uM + 2uM) - NT
|
0.0029221
|
0.0218523
|
44
|
0.1337214
|
0.9998749
|
|
(500nM + 500nM) - NT
|
-0.0290584
|
0.0218523
|
44
|
-1.3297644
|
0.5704859
|
[1] 8
HEC-1 3 Mix
Table 8
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
(1uM + 1uM) - NT
|
-0.0223269
|
0.0230973
|
44
|
-0.9666442
|
0.8091061
|
|
(250nM + 250nM) - NT
|
0.0024900
|
0.0230973
|
44
|
0.1078056
|
0.9999469
|
|
(2uM + 2uM) - NT
|
-0.0182554
|
0.0230973
|
44
|
-0.7903701
|
0.8970460
|
|
(500nM + 500nM) - NT
|
-0.0492565
|
0.0230973
|
44
|
-2.1325610
|
0.1456120
|
[1] 9
HEC-1 Carboplatin
Table 9
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
100 - NT
|
-0.4073890
|
0.028899
|
112
|
-14.096996
|
0.000000
|
|
200 - NT
|
-0.9236936
|
0.028899
|
112
|
-31.962834
|
0.000000
|
|
400 - NT
|
-0.9703006
|
0.028899
|
112
|
-33.575586
|
0.000000
|
|
50 - NT
|
-0.0668354
|
0.028899
|
112
|
-2.312724
|
0.087249
|
[1] 10
HEC-1 PFAS + Carb
Sheet 10: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 10 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0138881
|
0.0356161
|
33
|
-0.3899396
|
0.9992576
|
|
1% Methanol - 2μM PFOA
|
0.0030996
|
0.0356161
|
33
|
0.0870283
|
0.9999999
|
|
1% Methanol - 2μM PFPA
|
-0.0742050
|
0.0356161
|
33
|
-2.0834660
|
0.2415378
|
|
1% Methanol - 500nM PFHpA
|
-0.0145022
|
0.0356161
|
33
|
-0.4071806
|
0.9990507
|
|
1% Methanol - 500nM PFOA
|
0.0033127
|
0.0356161
|
33
|
0.0930124
|
0.9999998
|
|
1% Methanol - 500nM PFPA
|
-0.1056174
|
0.0356161
|
33
|
-2.9654372
|
0.0330184
|
Table 10 Exposure Group = 50
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
0.0059489
|
0.0339832
|
33
|
0.1750532
|
0.9999931
|
|
1% Methanol - 2μM PFOA
|
0.0008673
|
0.0339832
|
33
|
0.0255207
|
1.0000000
|
|
1% Methanol - 2μM PFPA
|
-0.0272489
|
0.0339832
|
33
|
-0.8018336
|
0.9651166
|
|
1% Methanol - 500nM PFHpA
|
0.0100557
|
0.0339832
|
33
|
0.2959032
|
0.9998487
|
|
1% Methanol - 500nM PFOA
|
0.0105774
|
0.0339832
|
33
|
0.3112546
|
0.9997970
|
|
1% Methanol - 500nM PFPA
|
-0.0452692
|
0.0339832
|
33
|
-1.3321032
|
0.7216326
|
Table 10 Exposure Group = 100
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0588928
|
0.0384912
|
33
|
-1.5300354
|
0.5826756
|
|
1% Methanol - 2μM PFOA
|
-0.0342812
|
0.0384912
|
33
|
-0.8906250
|
0.9429674
|
|
1% Methanol - 2μM PFPA
|
-0.1350320
|
0.0384912
|
33
|
-3.5081321
|
0.0079279
|
|
1% Methanol - 500nM PFHpA
|
-0.0276630
|
0.0384912
|
33
|
-0.7186850
|
0.9796272
|
|
1% Methanol - 500nM PFOA
|
0.0018919
|
0.0384912
|
33
|
0.0491506
|
1.0000000
|
|
1% Methanol - 500nM PFPA
|
-0.1496065
|
0.0384912
|
33
|
-3.8867760
|
0.0027775
|
Table 10 Exposure Group = 200
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0263482
|
0.0989299
|
33
|
-0.2663323
|
0.9999182
|
|
1% Methanol - 2μM PFOA
|
-0.1077494
|
0.0989299
|
33
|
-1.0891496
|
0.8652409
|
|
1% Methanol - 2μM PFPA
|
-0.2791820
|
0.0989299
|
33
|
-2.8220185
|
0.0471744
|
|
1% Methanol - 500nM PFHpA
|
0.0574493
|
0.0989299
|
33
|
0.5807073
|
0.9932603
|
|
1% Methanol - 500nM PFOA
|
0.0443647
|
0.0989299
|
33
|
0.4484462
|
0.9983648
|
|
1% Methanol - 500nM PFPA
|
-0.0090068
|
0.0989299
|
33
|
-0.0910426
|
0.9999999
|
Table 10 Exposure Group = 400
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.4194997
|
0.1395665
|
33
|
-3.0057340
|
0.0298169
|
|
1% Methanol - 2μM PFOA
|
-0.3644085
|
0.1395665
|
33
|
-2.6110025
|
0.0781806
|
|
1% Methanol - 2μM PFPA
|
-0.1100201
|
0.1395665
|
33
|
-0.7882985
|
0.9678643
|
|
1% Methanol - 500nM PFHpA
|
-0.2617518
|
0.1395665
|
33
|
-1.8754634
|
0.3513389
|
|
1% Methanol - 500nM PFOA
|
-0.1646082
|
0.1395665
|
33
|
-1.1794250
|
0.8172160
|
|
1% Methanol - 500nM PFPA
|
-0.1817480
|
0.1395665
|
33
|
-1.3022323
|
0.7414783
|
[1] 11
HEC-1 Mix + Carb
Sheet 11: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 11 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - (2uM PFHpA + 2uM PFPA)
|
-0.0358629
|
0.0526024
|
23
|
-0.6817722
|
0.9385885
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA)
|
-0.0524828
|
0.0526024
|
23
|
-0.9977264
|
0.7970338
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA + 2uM PFPA)
|
-0.0016691
|
0.0526024
|
23
|
-0.0317313
|
0.9999996
|
|
1% Methanol - (2uM PFOA + 2uM PFPA)
|
-0.0896743
|
0.0526024
|
23
|
-1.7047558
|
0.3488811
|
Table 11 Exposure Group = 50
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - (2uM PFHpA + 2uM PFPA)
|
-0.0163053
|
0.0328953
|
23
|
-0.4956711
|
0.9801886
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA)
|
-0.0050457
|
0.0328953
|
23
|
-0.1533861
|
0.9997887
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA + 2uM PFPA)
|
-0.0328564
|
0.0328953
|
23
|
-0.9988173
|
0.7964070
|
|
1% Methanol - (2uM PFOA + 2uM PFPA)
|
-0.0302051
|
0.0328953
|
23
|
-0.9182184
|
0.8404947
|
Table 11 Exposure Group = 100
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - (2uM PFHpA + 2uM PFPA)
|
-0.0202939
|
0.0254637
|
23
|
-0.7969742
|
0.8970937
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA)
|
-0.0009414
|
0.0254637
|
23
|
-0.0369705
|
0.9999993
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA + 2uM PFPA)
|
-0.0088327
|
0.0254637
|
23
|
-0.3468730
|
0.9948290
|
|
1% Methanol - (2uM PFOA + 2uM PFPA)
|
0.0077255
|
0.0254637
|
23
|
0.3033936
|
0.9969146
|
Table 11 Exposure Group = 200
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - (2uM PFHpA + 2uM PFPA)
|
0.0905287
|
0.0633987
|
23
|
1.4279272
|
0.5179429
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA)
|
-0.0832979
|
0.0633987
|
23
|
-1.3138750
|
0.5941616
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA + 2uM PFPA)
|
0.1000160
|
0.0633987
|
23
|
1.5775727
|
0.4226641
|
|
1% Methanol - (2uM PFOA + 2uM PFPA)
|
-0.0202636
|
0.0633987
|
23
|
-0.3196219
|
0.9962258
|
Table 11 Exposure Group = 400
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - (2uM PFHpA + 2uM PFPA)
|
0.0540236
|
0.0861131
|
23
|
0.6273563
|
0.9538888
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA)
|
-0.0104245
|
0.0861131
|
23
|
-0.1210553
|
0.9999175
|
|
1% Methanol - (2uM PFOA + 2uM PFHpA + 2uM PFPA)
|
0.1414874
|
0.0861131
|
23
|
1.6430418
|
0.3837172
|
|
1% Methanol - (2uM PFOA + 2uM PFPA)
|
-0.0963341
|
0.0861131
|
23
|
-1.1186922
|
0.7234434
|
[1] 12
HEC-1 Cisplatin
Sheet 12 Please compare all groups to NT.
Table 12
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
0.1 - NT
|
-0.0023543
|
0.0362263
|
168
|
-0.0649883
|
1.0000000
|
|
0.25 - NT
|
-0.0563004
|
0.0362263
|
168
|
-1.5541285
|
0.6900495
|
|
0.5 - NT
|
-0.0382453
|
0.0362263
|
168
|
-1.0557326
|
0.9556441
|
|
1 - NT
|
-0.0626876
|
0.0362263
|
168
|
-1.7304426
|
0.5521484
|
|
10 - NT
|
-0.7276880
|
0.0362263
|
168
|
-20.0872744
|
0.0000000
|
|
2.5 - NT
|
-0.1739174
|
0.0362263
|
168
|
-4.8008571
|
0.0000313
|
|
25 - NT
|
-0.8996482
|
0.0362263
|
168
|
-24.8341053
|
0.0000000
|
|
5 - NT
|
-0.3910903
|
0.0362263
|
168
|
-10.7957498
|
0.0000000
|
|
50 - NT
|
-0.9578774
|
0.0362263
|
168
|
-26.4414796
|
0.0000000
|
[1] 13
HEC-1 PFAS + Cis
Sheet 13: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 13 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
0.0058141
|
0.0311425
|
44
|
0.1866923
|
0.9999898
|
|
1% Methanol - 2μM PFOA
|
0.0009543
|
0.0284951
|
44
|
0.0334911
|
1.0000000
|
|
1% Methanol - 2μM PFPA
|
-0.0250362
|
0.0284951
|
44
|
-0.8786133
|
0.9455673
|
|
1% Methanol - 500nM PFHpA
|
-0.0069544
|
0.0284951
|
44
|
-0.2440563
|
0.9999504
|
|
1% Methanol - 500nM PFOA
|
0.0097554
|
0.0284951
|
44
|
0.3423521
|
0.9996435
|
|
1% Methanol - 500nM PFPA
|
-0.0694288
|
0.0284951
|
44
|
-2.4365158
|
0.1084197
|
Table 13 Exposure Group = 2.5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0440890
|
0.0272906
|
46
|
-1.6155391
|
0.5130943
|
|
1% Methanol - 2μM PFOA
|
-0.0545977
|
0.0272906
|
46
|
-2.0006073
|
0.2711857
|
|
1% Methanol - 2μM PFPA
|
-0.0204011
|
0.0272906
|
46
|
-0.7475512
|
0.9747989
|
|
1% Methanol - 500nM PFHpA
|
-0.0289228
|
0.0272906
|
46
|
-1.0598115
|
0.8769708
|
|
1% Methanol - 500nM PFOA
|
0.0086279
|
0.0272906
|
46
|
0.3161512
|
0.9997747
|
|
1% Methanol - 500nM PFPA
|
-0.0368658
|
0.0272906
|
46
|
-1.3508624
|
0.7033635
|
Table 13 Exposure Group = 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0067547
|
0.0325639
|
40
|
-0.2074293
|
0.9999811
|
|
1% Methanol - 2μM PFOA
|
-0.0535377
|
0.0325639
|
40
|
-1.6440797
|
0.4962729
|
|
1% Methanol - 2μM PFPA
|
0.0221139
|
0.0356849
|
40
|
0.6196973
|
0.9903979
|
|
1% Methanol - 500nM PFHpA
|
-0.0332783
|
0.0356109
|
40
|
-0.9344961
|
0.9284336
|
|
1% Methanol - 500nM PFOA
|
0.0274417
|
0.0325639
|
40
|
0.8427031
|
0.9553620
|
|
1% Methanol - 500nM PFPA
|
-0.0019823
|
0.0356849
|
40
|
-0.0555505
|
1.0000000
|
Table 13 Exposure Group = 10
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.0156073
|
0.0566781
|
46
|
-0.2753669
|
0.9998992
|
|
1% Methanol - 2μM PFOA
|
-0.0041641
|
0.0566781
|
46
|
-0.0734693
|
1.0000000
|
|
1% Methanol - 2μM PFPA
|
-0.0306473
|
0.0566781
|
46
|
-0.5407258
|
0.9953399
|
|
1% Methanol - 500nM PFHpA
|
0.0106935
|
0.0566781
|
46
|
0.1886712
|
0.9999891
|
|
1% Methanol - 500nM PFOA
|
0.0343481
|
0.0566781
|
46
|
0.6060207
|
0.9914133
|
|
1% Methanol - 500nM PFPA
|
-0.0998881
|
0.0566781
|
46
|
-1.7623759
|
0.4118007
|
Table 13 Exposure Group = 25
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.1864192
|
0.0862553
|
33
|
-2.1612491
|
0.2075544
|
|
1% Methanol - 2μM PFOA
|
-0.0729312
|
0.0862553
|
33
|
-0.8455273
|
0.9551368
|
|
1% Methanol - 2μM PFPA
|
-0.0031281
|
0.0862553
|
33
|
-0.0362654
|
1.0000000
|
|
1% Methanol - 500nM PFHpA
|
-0.0633499
|
0.0862553
|
33
|
-0.7344461
|
0.9772942
|
|
1% Methanol - 500nM PFOA
|
-0.0466990
|
0.0862553
|
33
|
-0.5414048
|
0.9953781
|
|
1% Methanol - 500nM PFPA
|
0.0331995
|
0.0862553
|
33
|
0.3848982
|
0.9993106
|
[1] 14
HEC-1 Mix + Cis
Sheet 14: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 14 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
0.0244381
|
0.0376996
|
30
|
0.6482325
|
0.9476926
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.0002825
|
0.0342960
|
30
|
-0.0082371
|
1.0000000
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.0175792
|
0.0342960
|
30
|
-0.5125737
|
0.9773372
|
|
1% Methanol - (PFOA + PFPA)
|
-0.0039698
|
0.0342960
|
30
|
-0.1157521
|
0.9999303
|
Table 14 Exposure Group = 2.5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
0.0134387
|
0.0306790
|
30
|
0.4380439
|
0.9873291
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.0133542
|
0.0279092
|
30
|
-0.4784859
|
0.9824010
|
|
1% Methanol - (PFOA + PFHpA)
|
0.0124234
|
0.0279092
|
30
|
0.4451354
|
0.9865448
|
|
1% Methanol - (PFOA + PFPA)
|
0.0386638
|
0.0279092
|
30
|
1.3853410
|
0.5393564
|
Table 14 Exposure Group = 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
0.0045173
|
0.0459372
|
23
|
0.0983374
|
0.9999640
|
|
1% Methanol - (PFHpA + PFPA)
|
0.0328104
|
0.0459372
|
23
|
0.7142445
|
0.9281480
|
|
1% Methanol - (PFOA + PFHpA)
|
0.0554814
|
0.0459372
|
23
|
1.2077643
|
0.6653429
|
|
1% Methanol - (PFOA + PFPA)
|
0.0896236
|
0.0459372
|
23
|
1.9510006
|
0.2302978
|
Table 14 Exposure Group = 10
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.0099428
|
0.0786576
|
26
|
-0.1264061
|
0.9999015
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.0408908
|
0.0710723
|
26
|
-0.5753415
|
0.9658141
|
|
1% Methanol - (PFOA + PFHpA)
|
0.1502026
|
0.0786576
|
26
|
1.9095752
|
0.2431597
|
|
1% Methanol - (PFOA + PFPA)
|
0.0726358
|
0.0782526
|
26
|
0.9282216
|
0.8341388
|
Table 14 Exposure Group = 25
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.0257967
|
0.0738125
|
23
|
-0.3494891
|
0.9946779
|
|
1% Methanol - (PFHpA + PFPA)
|
0.0465919
|
0.0738125
|
23
|
0.6312195
|
0.9528920
|
|
1% Methanol - (PFOA + PFHpA)
|
0.0077677
|
0.0738125
|
23
|
0.1052350
|
0.9999528
|
|
1% Methanol - (PFOA + PFPA)
|
0.0619500
|
0.0738125
|
23
|
0.8392886
|
0.8787824
|
[1] 15
HEC-1 PFAS + Carb JC-1
Sheet 15: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 15 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.3312848
|
0.0720129
|
36
|
-4.600354
|
0.0003036
|
|
1% Methanol - 2μM PFOA
|
-0.2128260
|
0.0708819
|
36
|
-3.002544
|
0.0287186
|
|
1% Methanol - 2μM PFPA
|
-0.2345000
|
0.0720129
|
36
|
-3.256361
|
0.0146820
|
|
1% Methanol - 500nM PFHpA
|
-0.2645569
|
0.0715626
|
36
|
-3.696858
|
0.0043284
|
|
1% Methanol - 500nM PFOA
|
-0.0723389
|
0.0653197
|
36
|
-1.107458
|
0.8553084
|
|
1% Methanol - 500nM PFPA
|
-0.1629574
|
0.0720129
|
36
|
-2.262892
|
0.1658509
|
Table 15 Exposure Group = 50
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.4405388
|
0.0719125
|
31
|
-6.126036
|
0.0000051
|
|
1% Methanol - 2μM PFOA
|
-0.1701613
|
0.0719125
|
31
|
-2.366226
|
0.1377620
|
|
1% Methanol - 2μM PFPA
|
-0.2079080
|
0.0719125
|
31
|
-2.891123
|
0.0410117
|
|
1% Methanol - 500nM PFHpA
|
-0.2282878
|
0.0817297
|
31
|
-2.793206
|
0.0520258
|
|
1% Methanol - 500nM PFOA
|
-0.0820501
|
0.0719125
|
31
|
-1.140971
|
0.8392511
|
|
1% Methanol - 500nM PFPA
|
-0.1394695
|
0.0719125
|
31
|
-1.939433
|
0.3171276
|
Table 15 Exposure Group = 100
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.2570606
|
0.0879932
|
33
|
-2.921370
|
0.0368829
|
|
1% Methanol - 2μM PFOA
|
-0.0984905
|
0.0879932
|
33
|
-1.119298
|
0.8500219
|
|
1% Methanol - 2μM PFPA
|
-0.2848677
|
0.0879932
|
33
|
-3.237385
|
0.0163721
|
|
1% Methanol - 500nM PFHpA
|
-0.2659144
|
0.0879932
|
33
|
-3.021990
|
0.0286092
|
|
1% Methanol - 500nM PFOA
|
-0.1067377
|
0.0879932
|
33
|
-1.213023
|
0.7975675
|
|
1% Methanol - 500nM PFPA
|
-0.1549694
|
0.0879932
|
33
|
-1.761152
|
0.4226194
|
Table 15 Exposure Group = 200
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.2587417
|
0.0712918
|
32
|
-3.6293350
|
0.0058633
|
|
1% Methanol - 2μM PFOA
|
-0.1175582
|
0.0712918
|
32
|
-1.6489731
|
0.4994489
|
|
1% Methanol - 2μM PFPA
|
-0.1810169
|
0.0749771
|
32
|
-2.4142944
|
0.1231227
|
|
1% Methanol - 500nM PFHpA
|
-0.2825728
|
0.0712918
|
32
|
-3.9636106
|
0.0023251
|
|
1% Methanol - 500nM PFOA
|
-0.1349088
|
0.0712918
|
32
|
-1.8923483
|
0.3425957
|
|
1% Methanol - 500nM PFPA
|
-0.0582542
|
0.0712918
|
32
|
-0.8171238
|
0.9618919
|
Table 15 Exposure Group = 400
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.2437957
|
0.0576481
|
31
|
-4.2290348
|
0.0011547
|
|
1% Methanol - 2μM PFOA
|
-0.0461060
|
0.0576481
|
31
|
-0.7997831
|
0.9656756
|
|
1% Methanol - 2μM PFPA
|
-0.0345655
|
0.0576481
|
31
|
-0.5995945
|
0.9920372
|
|
1% Methanol - 500nM PFHpA
|
-0.2481961
|
0.0576481
|
31
|
-4.3053671
|
0.0009314
|
|
1% Methanol - 500nM PFOA
|
-0.0640718
|
0.0576481
|
31
|
-1.1114306
|
0.8546941
|
|
1% Methanol - 500nM PFPA
|
-0.0634579
|
0.0655179
|
31
|
-0.9685578
|
0.9175429
|
Table 15 Exposure Group = 2.5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.6242884
|
0.1400811
|
5
|
-4.456621
|
0.0263801
|
|
1% Methanol - 2μM PFOA
|
-0.4502676
|
0.1400811
|
5
|
-3.214335
|
0.0911637
|
|
1% Methanol - 500nM PFHpA
|
-0.2004353
|
0.1400811
|
5
|
-1.430852
|
0.6141936
|
|
1% Methanol - 500nM PFOA
|
-0.0402110
|
0.1400811
|
5
|
-0.287055
|
0.9978864
|
Table 15 Exposure Group = 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 500nM PFHpA
|
-0.3958188
|
0.1238922
|
4
|
-3.1948636
|
0.0959439
|
|
1% Methanol - 500nM PFOA
|
-0.2106237
|
0.1238922
|
4
|
-1.7000562
|
0.4164441
|
|
1% Methanol - 500nM PFPA
|
-0.0197903
|
0.1238922
|
4
|
-0.1597383
|
0.9983076
|
Table 15 Exposure Group = 10
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFPA
|
0.0990117
|
0.1893989
|
3
|
0.522768
|
0.9522815
|
|
1% Methanol - 500nM PFHpA
|
-0.2469722
|
0.1546436
|
3
|
-1.597041
|
0.5042207
|
|
1% Methanol - 500nM PFOA
|
-0.2132405
|
0.1546436
|
3
|
-1.378916
|
0.5976365
|
Table 15 Exposure Group = 25
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 500nM PFOA
|
-0.1437960
|
0.2144233
|
3
|
-0.6706173
|
0.7979270
|
|
1% Methanol - 500nM PFPA
|
-0.1279724
|
0.2144233
|
3
|
-0.5968213
|
0.8340791
|
[1] 16
HEC-1 Mix + Carb JC-1
Sheet 16: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 16 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1%. Methanol - 3 Mix
|
-0.3225043
|
0.0738533
|
32
|
-4.366825
|
0.0004947
|
|
1%. Methanol - (PFHpA + PFPA)
|
-0.1596181
|
0.0738533
|
32
|
-2.161287
|
0.1444859
|
|
1%. Methanol - (PFOA + PFHpA)
|
-0.1229052
|
0.0738533
|
32
|
-1.664181
|
0.3607647
|
|
1%. Methanol - (PFOA + PFPA)
|
-0.1730408
|
0.0738533
|
32
|
-2.343034
|
0.0981883
|
Table 16 Exposure Group = 50
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1%. Methanol - 3 Mix
|
-0.1799488
|
0.0679539
|
32
|
-2.648101
|
0.0489225
|
|
1%. Methanol - (PFHpA + PFPA)
|
-0.1075235
|
0.0679539
|
32
|
-1.582302
|
0.4095610
|
|
1%. Methanol - (PFOA + PFHpA)
|
-0.1165675
|
0.0679539
|
32
|
-1.715392
|
0.3319890
|
|
1%. Methanol - (PFOA + PFPA)
|
-0.1293302
|
0.0679539
|
32
|
-1.903206
|
0.2391336
|
Table 16 Exposure Group = 100
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1%. Methanol - 3 Mix
|
-0.3047886
|
0.0603869
|
23
|
-5.0472633
|
0.0001659
|
|
1%. Methanol - (PFHpA + PFPA)
|
-0.2591950
|
0.0603869
|
23
|
-4.2922380
|
0.0010867
|
|
1%. Methanol - (PFOA + PFHpA)
|
-0.0493981
|
0.0603869
|
23
|
-0.8180262
|
0.8881843
|
|
1%. Methanol - (PFOA + PFPA)
|
-0.1482424
|
0.0603869
|
23
|
-2.4548758
|
0.0854331
|
Table 16 Exposure Group = 200
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1%. Methanol - 3 Mix
|
-0.1814603
|
0.0703011
|
30
|
-2.5811888
|
0.0585816
|
|
1%. Methanol - (PFHpA + PFPA)
|
-0.1536157
|
0.0772779
|
30
|
-1.9878345
|
0.2059565
|
|
1%. Methanol - (PFOA + PFHpA)
|
-0.0699366
|
0.0703011
|
30
|
-0.9948162
|
0.7958077
|
|
1%. Methanol - (PFOA + PFPA)
|
-0.1877213
|
0.0703011
|
30
|
-2.6702477
|
0.0476067
|
Table 16 Exposure Group = 400
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1%. Methanol - 3 Mix
|
-0.2009115
|
0.0581869
|
26
|
-3.452865
|
0.0076245
|
|
1%. Methanol - (PFHpA + PFPA)
|
-0.1413840
|
0.0643969
|
26
|
-2.195508
|
0.1408781
|
|
1%. Methanol - (PFOA + PFHpA)
|
-0.0787800
|
0.0640654
|
26
|
-1.229680
|
0.6481569
|
|
1%. Methanol - (PFOA + PFPA)
|
-0.0878230
|
0.0643969
|
26
|
-1.363776
|
0.5573520
|
[1] 17
HEC-1 PFAS + Cis JC-1
Sheet 17: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 17 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.1716195
|
0.0664848
|
42
|
-2.5813334
|
0.0778855
|
|
1% Methanol - 2μM PFOA
|
-0.1007547
|
0.0664848
|
42
|
-1.5154543
|
0.5873159
|
|
1% Methanol - 2μM PFPA
|
-0.1010978
|
0.0728305
|
42
|
-1.3881250
|
0.6787345
|
|
1% Methanol - 500nM PFHpA
|
-0.0232003
|
0.0664848
|
42
|
-0.3489558
|
0.9996027
|
|
1% Methanol - 500nM PFOA
|
-0.0835774
|
0.0664848
|
42
|
-1.2570897
|
0.7671895
|
|
1% Methanol - 500nM PFPA
|
-0.0313102
|
0.0728305
|
42
|
-0.4299057
|
0.9986958
|
Table 17 Exposure Group = 2.5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.2693473
|
0.0891460
|
38
|
-3.0214195
|
0.0266064
|
|
1% Methanol - 2μM PFOA
|
-0.0956453
|
0.0806356
|
38
|
-1.1861428
|
0.8117141
|
|
1% Methanol - 2μM PFPA
|
-0.1783946
|
0.0891460
|
38
|
-2.0011514
|
0.2766933
|
|
1% Methanol - 500nM PFHpA
|
-0.1600108
|
0.0891460
|
38
|
-1.7949306
|
0.3961010
|
|
1% Methanol - 500nM PFOA
|
-0.0561793
|
0.0806356
|
38
|
-0.6967057
|
0.9824507
|
|
1% Methanol - 500nM PFPA
|
-0.0523762
|
0.0891460
|
38
|
-0.5875331
|
0.9927754
|
Table 17 Exposure Group = 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.1464649
|
0.0776241
|
33
|
-1.8868481
|
0.3446360
|
|
1% Methanol - 2μM PFOA
|
-0.1011991
|
0.0776241
|
33
|
-1.3037072
|
0.7405094
|
|
1% Methanol - 2μM PFPA
|
-0.1340580
|
0.0776241
|
33
|
-1.7270148
|
0.4451686
|
|
1% Methanol - 500nM PFHpA
|
-0.0592349
|
0.0776241
|
33
|
-0.7630991
|
0.9725677
|
|
1% Methanol - 500nM PFOA
|
-0.0487326
|
0.0776241
|
33
|
-0.6278028
|
0.9898193
|
|
1% Methanol - 500nM PFPA
|
0.0871076
|
0.0776241
|
33
|
1.1221724
|
0.8485265
|
Table 17 Exposure Group = 10
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.1776657
|
0.0674919
|
33
|
-2.6324000
|
0.0743644
|
|
1% Methanol - 2μM PFOA
|
-0.0305873
|
0.0674919
|
33
|
-0.4531992
|
0.9982654
|
|
1% Methanol - 2μM PFPA
|
-0.0300841
|
0.0674919
|
33
|
-0.4457440
|
0.9984192
|
|
1% Methanol - 500nM PFHpA
|
-0.0621340
|
0.0674919
|
33
|
-0.9206141
|
0.9337759
|
|
1% Methanol - 500nM PFOA
|
-0.0416335
|
0.0674919
|
33
|
-0.6168665
|
0.9907163
|
|
1% Methanol - 500nM PFPA
|
-0.0301288
|
0.0674919
|
33
|
-0.4464054
|
0.9984060
|
Table 17 Exposure Group = 25
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 2μM PFHpA
|
-0.1538523
|
0.0815506
|
33
|
-1.8865874
|
0.3447887
|
|
1% Methanol - 2μM PFOA
|
-0.1451057
|
0.0815506
|
33
|
-1.7793337
|
0.4108287
|
|
1% Methanol - 2μM PFPA
|
-0.0789266
|
0.0815506
|
33
|
-0.9678234
|
0.9174757
|
|
1% Methanol - 500nM PFHpA
|
-0.0615402
|
0.0815506
|
33
|
-0.7546259
|
0.9740325
|
|
1% Methanol - 500nM PFOA
|
0.0742982
|
0.0815506
|
33
|
0.9110688
|
0.9367985
|
|
1% Methanol - 500nM PFPA
|
-0.0330111
|
0.0815506
|
33
|
-0.4047933
|
0.9990819
|
[1] 18
HEC-1 Mix + Cis JC-1
Sheet 18: Please compare each PFAS exposure group to the methanol
group for each carboplatin dose.
Table 18 Exposure Group = 0
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.1974941
|
0.0649921
|
32
|
-3.0387385
|
0.0186849
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.1707606
|
0.0649921
|
32
|
-2.6274048
|
0.0513770
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.0586633
|
0.0649921
|
32
|
-0.9026209
|
0.8459141
|
|
1% Methanol - (PFOA + PFPA)
|
-0.0554503
|
0.0649921
|
32
|
-0.8531841
|
0.8703123
|
Table 18 Exposure Group = 2.5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.2052735
|
0.0904113
|
23
|
-2.270442
|
0.1251104
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.1463094
|
0.0904113
|
23
|
-1.618265
|
0.3982269
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.1432127
|
0.0904113
|
23
|
-1.584014
|
0.4187474
|
|
1% Methanol - (PFOA + PFPA)
|
-0.1126822
|
0.0904113
|
23
|
-1.246330
|
0.6396008
|
Table 18 Exposure Group = 5
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.2166618
|
0.0979404
|
32
|
-2.212181
|
0.1299860
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.2295020
|
0.0979404
|
32
|
-2.343283
|
0.0981349
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.1604607
|
0.0979404
|
32
|
-1.638351
|
0.3758015
|
|
1% Methanol - (PFOA + PFPA)
|
-0.1796710
|
0.0979404
|
32
|
-1.834493
|
0.2707302
|
Table 18 Exposure Group = 10
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.0630341
|
0.0758708
|
32
|
-0.8308081
|
0.8806547
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.0533005
|
0.0758708
|
32
|
-0.7025166
|
0.9309774
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.1065539
|
0.0758708
|
32
|
-1.4044131
|
0.5250238
|
|
1% Methanol - (PFOA + PFPA)
|
-0.0565857
|
0.0758708
|
32
|
-0.7458170
|
0.9157364
|
Table 18 Exposure Group = 25
|
contrast
|
estimate
|
SE
|
df
|
t.ratio
|
p.value
|
|
1% Methanol - 3 Mix
|
-0.0728180
|
0.0769863
|
32
|
-0.9458565
|
0.8229281
|
|
1% Methanol - (PFHpA + PFPA)
|
-0.0483934
|
0.0769863
|
32
|
-0.6285969
|
0.9528734
|
|
1% Methanol - (PFOA + PFHpA)
|
-0.0475797
|
0.0769863
|
32
|
-0.6180278
|
0.9555868
|
|
1% Methanol - (PFOA + PFPA)
|
-0.0072147
|
0.0769863
|
32
|
-0.0937143
|
0.9999699
|
[1] 19