Background

Attached are data files for your analysis. I have specified in each sheet what the requested comparisons are. They are very similar to those that we’ve sent you previously for our first paper together. I totally understand if you can’t get through all data before the holidays, but if you could prioritize the HEC-1 data file, that would be great. Please feel free to reach out if you have any questions!

list of all files and sheets

4 excel files

  • File 1 (Ishikawa PFAS + Carboplatin.xlsx): with 1 sheet in total …
    • Sheet 1: Please compare each exposure group to the methanol group at each carboplatin dose.
  • File 2 (05.01.2023 HEC-1 Data for Xianming FINAL.xlsx): with 9 sheets in total …
    • Sheet 1: Please compare each treatment group to the 0 Carb group
  • File 3 (Ishikawa Data for Xianming.xlsx): with 12 sheets in total …
  • File 4 (OvCa mtDNA and ROS Data for Xianming.xlsx) with 12 sheet2 in total …

For file 3: “Ishikawa Data for Xianming.xlsx”

  • There are length(mysheets) sheets in this file
  • Sheet 1: *Please compare each exposure group to the no methanol control
    Table 1
    contrast estimate SE df t.ratio p.value
    Exposure0 - Exposure0.001 -0.0100323 0.0410747 227 -0.2442454 0.9999996
    Exposure0 - Exposure0.0025 0.0092482 0.0410747 227 0.2251565 0.9999998
    Exposure0 - Exposure0.005 0.0383667 0.0410747 227 0.9340731 0.9796463
    Exposure0 - Exposure0.0075 0.0500631 0.0410747 227 1.2188316 0.8981683
    Exposure0 - Exposure0.01 0.1092460 0.0410747 227 2.6596925 0.0729296
    Exposure0 - Exposure0.02 0.3906474 0.0410747 227 9.5106649 0.0000000
    Exposure0 - Exposure0.03 0.7902033 0.0410747 227 19.2382164 0.0000000
    Exposure0 - Exposure0.04 0.9271902 0.0410747 227 22.5732859 0.0000000
    Exposure0 - Exposure0.05 0.9613772 0.0410747 227 23.4056008 0.0000000

[1] 2

  • Sheet 2: *Please compare each exposure group to the methanol group
    Table 2
    contrast estimate SE df t.ratio p.value
    100nM PFHpA - Methanol 0.0276327 0.0449616 148 0.6145829 1.0000000
    100nM PFOA - Methanol -0.0334803 0.0449616 148 -0.7446420 1.0000000
    100nM PFPA - Methanol 0.0265712 0.0449616 148 0.5909740 1.0000000
    1uM PFHpA - Methanol 0.0807185 0.0449616 148 1.7952743 0.8945934
    1uM PFOA - Methanol 0.0380132 0.0449616 148 0.8454590 0.9999996
    1uM PFPA - Methanol 0.0690877 0.0449616 148 1.5365931 0.9802203
    250nM PFHpA - Methanol 0.1155155 0.0449616 148 2.5692021 0.2782738
    250nM PFOA - Methanol 0.0084402 0.0449616 148 0.1877201 1.0000000
    250nM PFPA - Methanol 0.0903482 0.0449616 148 2.0094513 0.7471589
    25nM PFHpA - Methanol 0.0537177 0.0449616 148 1.1947463 0.9995624
    25nM PFOA - Methanol -0.0828108 0.0449616 148 -1.8418112 0.8682479
    25nM PFPA - Methanol 0.0828830 0.0449616 148 1.8434168 0.8672773
    2uM PFHpA - Methanol 0.0100693 0.0521405 148 0.1931185 1.0000000
    2uM PFOA - Methanol 0.0657013 0.0449616 148 1.4612760 0.9897340
    2uM PFPA - Methanol 0.0241396 0.0449616 148 0.5368928 1.0000000
    2uM PFPFHpA - Methanol 0.1044711 0.0693534 148 1.5063599 0.9846371
    500nM PFHpA - Methanol 0.0626943 0.0449616 148 1.3943961 0.9946964
    500nM PFOA - Methanol 0.0321541 0.0449616 148 0.7151461 1.0000000
    500nM PFPA - Methanol -0.0051232 0.0449616 148 -0.1139455 1.0000000
    50nM PFHpA - Methanol 0.1151275 0.0449616 148 2.5605713 0.2839509
    50nM PFOA - Methanol -0.0623692 0.0449616 148 -1.3871655 0.9950853
    50nM PFPA - Methanol 0.1691863 0.0449616 148 3.7629041 0.0069794
    750nM PFHpA - Methanol 0.0546664 0.0449616 148 1.2158464 0.9994059
    750nM PFOA - Methanol 0.0106323 0.0449616 148 0.2364751 1.0000000
    750nM PFPA - Methanol 0.0726365 0.0449616 148 1.6155225 0.9640286
    75nM PFHpA - Methanol 0.0863021 0.0449616 148 1.9194610 0.8168364
    75nM PFOA - Methanol -0.0346726 0.0449616 148 -0.7711601 1.0000000
    75nM PFPA - Methanol 0.0590219 0.0449616 148 1.3127180 0.9978832
    Media - Methanol -0.0166364 0.0449616 148 -0.3700142 1.0000000

[1] 3

  • Sheet 3: *Please compare each exposure group to the methanol group
    Table 3
    contrast estimate SE df t.ratio p.value
    (100nM PFOA + 100nM PFHpA + 100nM PFPA) - Methanol -0.0670298 0.0367459 160 -1.8241422 0.7087674
    (1uM PFHpA + 1uM PFPA) - Methanol 0.0232806 0.0367459 160 0.6335575 0.9999971
    (1uM PFOA + 1uM PFHpA) - Methanol 0.0237249 0.0367459 160 0.6456468 0.9999961
    (1uM PFOA + 1uM PFPA) - Methanol 0.0211996 0.0367459 160 0.5769240 0.9999993
    (250nM PFHpA + 250nM PFPA) - Methanol -0.0628425 0.0367459 160 -1.7101895 0.7956187
    (250nM PFOA + 250nM PFHpA) - Methanol -0.0784374 0.0367459 160 -2.1345883 0.4476897
    (250nM PFOA + 250nM PFHpA + 250nM PFPA) - Methanol 0.0431390 0.0367459 160 1.1739824 0.9910268
    (250nM PFOA + 250nM PFPA) - Methanol -0.0731245 0.0367459 160 -1.9900032 0.5689316
    (500nM PFHpA + 500nM PFPA) - Methanol 0.0143309 0.0367459 160 0.3899998 1.0000000
    (500nM PFOA + 500nM PFHpA) - Methanol 0.0175279 0.0367459 160 0.4770023 1.0000000
    (500nM PFOA + 500nM PFHpA + 500nM PFPA) - Methanol -0.0641257 0.0367459 160 -1.7451123 0.7702779
    (500nM PFOA + 500nM PFPA) - Methanol 0.0279259 0.0367459 160 0.7599718 0.9999595
    (750nM PFHpA + 750nM PFPA) - Methanol -0.0511057 0.0367459 160 -1.3907853 0.9545159
    (750nM PFOA + 750nM PFHpA) - Methanol -0.0895712 0.0367459 160 -2.4375833 0.2382563
    (750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.0177302 0.0367459 160 0.4825088 1.0000000
    (750nM PFOA + 750nM PFPA) - Methanol -0.0809809 0.0367459 160 -2.2038071 0.3932863
    Media - Methanol -0.0169609 0.0300029 160 -0.5653077 0.9999995

[1] 4

  • Sheet 4: *Please compare each treatment group to the no carboplatin group
    Table 4
    contrast estimate SE df t.ratio p.value
    Exposure0 - Exposure1 0.0367565 0.0331278 226 1.1095358 0.9560594
    Exposure0 - Exposure2.5 -0.0176277 0.0265614 226 -0.6636589 0.9991619
    Exposure0 - Exposure5 -0.0160295 0.0265614 226 -0.6034884 0.9996344
    Exposure0 - Exposure10 0.0008766 0.0265614 226 0.0330013 1.0000000
    Exposure0 - Exposure25 -0.0001442 0.0265614 226 -0.0054293 1.0000000
    Exposure0 - Exposure50 0.0031642 0.0265614 226 0.1191271 1.0000000
    Exposure0 - Exposure100 0.0894951 0.0265614 226 3.3693694 0.0088223
    Exposure0 - Exposure200 0.3139493 0.0265614 226 11.8197707 0.0000000
    Exposure0 - Exposure400 0.8030669 0.0265614 226 30.2343957 0.0000000
    Exposure0 - Exposure800 0.9142212 0.0331278 226 27.5967593 0.0000000

[1] 5

  • Sheet 5: *Please compare each treatment group to the no cisplatin group
    Table 5
    contrast estimate SE df t.ratio p.value
    Exposure0 - Exposure0.1 -0.0326408 0.0195135 227 -1.6727342 0.5958288
    Exposure0 - Exposure0.25 -0.0538138 0.0195135 227 -2.7577780 0.0552360
    Exposure0 - Exposure0.5 -0.0382401 0.0195135 227 -1.9596790 0.3772171
    Exposure0 - Exposure1 -0.0247588 0.0195135 227 -1.2688037 0.8743054
    Exposure0 - Exposure2.5 0.0164299 0.0195135 227 0.8419788 0.9900255
    Exposure0 - Exposure5 0.1461859 0.0195135 227 7.4915388 0.0000000
    Exposure0 - Exposure10 0.4401243 0.0195135 227 22.5549057 0.0000000
    Exposure0 - Exposure25 0.8956105 0.0195135 227 45.8970564 0.0000000
    Exposure0 - Exposure50 0.9576908 0.0195135 227 49.0784623 0.0000000

[1] 6

  • Sheet 6: *Please compare each exposure group to the methanol group at each cisplatin dose
Table 6 Exposure = 0uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol -0.0595094 0.0376391 46 -1.5810529 0.5378658
2uM PFOA - Methanol -0.0013875 0.0376391 46 -0.0368620 1.0000000
2uM PFPA - Methanol 0.0302609 0.0376391 46 0.8039743 0.9640643
500nM PFHpA - Methanol 0.0035478 0.0376391 46 0.0942582 0.9999998
500nM PFOA - Methanol 0.0241410 0.0376391 46 0.6413807 0.9884354
500nM PFPA - Methanol 0.0133069 0.0376391 46 0.3535391 0.9995703
Table 6 Exposure = 2.5uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.0303238 0.0389556 46 0.7784190 0.9692606
2uM PFOA - Methanol 0.0140477 0.0389556 46 0.3606081 0.9995186
2uM PFPA - Methanol 0.0504158 0.0389556 46 1.2941855 0.7418825
500nM PFHpA - Methanol 0.0407771 0.0389556 46 1.0467569 0.8830360
500nM PFOA - Methanol 0.0471300 0.0389556 46 1.2098385 0.7956396
500nM PFPA - Methanol 0.0113984 0.0389556 46 0.2925994 0.9998563
Table 6 Exposure = 5uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.0484401 0.0407581 46 1.1884792 0.8084297
2uM PFOA - Methanol 0.0825431 0.0407581 46 2.0251946 0.2587259
2uM PFPA - Methanol 0.0288949 0.0407581 46 0.7089378 0.9806680
500nM PFHpA - Methanol 0.0540536 0.0407581 46 1.3262053 0.7203191
500nM PFOA - Methanol 0.0776343 0.0407581 46 1.9047584 0.3235612
500nM PFPA - Methanol -0.0258453 0.0407581 46 -0.6341143 0.9891029
Table 6 Exposure = 10uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.0030773 0.0591348 46 0.0520379 1.0000000
2uM PFOA - Methanol -0.0107052 0.0591348 46 -0.1810308 0.9999915
2uM PFPA - Methanol -0.0147509 0.0591348 46 -0.2494449 0.9999435
500nM PFHpA - Methanol 0.0749930 0.0591348 46 1.2681695 0.7589702
500nM PFOA - Methanol 0.0182600 0.0591348 46 0.3087868 0.9998035
500nM PFPA - Methanol -0.0266626 0.0591348 46 -0.4508776 0.9982901
Table 6 Exposure = 25uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol -0.0337596 0.0891251 46 -0.3787886 0.9993619
2uM PFOA - Methanol -0.0298878 0.0891251 46 -0.3353463 0.9996831
2uM PFPA - Methanol 0.0770337 0.0891251 46 0.8643319 0.9494303
500nM PFHpA - Methanol 0.0213053 0.0891251 46 0.2390493 0.9999560
500nM PFOA - Methanol -0.0630855 0.0891251 46 -0.7078308 0.9808199
500nM PFPA - Methanol -0.0350038 0.0891251 46 -0.3927491 0.9992157

[1] 7

  • Sheet 7: *Pleasecompare each exposure group to the methanol group at each carboplatin dose
    Table 7 Exposure = 0uM Carboplatin
    contrast estimate SE df t.ratio p.value
    (1uM PFHpA + 1uM PFPA) - Methanol 0.0691307 0.0420998 39 1.6420697 0.3686660
    (1uM PFOA + 1uM PFHpA) - Methanol 0.0239412 0.0420998 39 0.5686773 0.9667049
    (1uM PFOA + 1uM PFPA) - Methanol -0.0205864 0.0420998 39 -0.4889908 0.9807647
    (750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.0243304 0.0420998 39 -0.5779219 0.9647305
Table 7 Exposure = 50uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.0116741 0.0486644 41 0.2398891 0.9987404
(1uM PFOA + 1uM PFHpA) - Methanol 0.0868606 0.0486644 41 1.7848896 0.2888236
(1uM PFOA + 1uM PFPA) - Methanol -0.0005765 0.0486644 41 -0.0118468 1.0000000
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.0458390 0.0486644 41 -0.9419406 0.8233979
Table 7 Exposure = 100uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol -0.1751912 0.0700558 41 -2.5007382 0.0643196
(1uM PFOA + 1uM PFHpA) - Methanol -0.0017662 0.0700558 41 -0.0252118 0.9999998
(1uM PFOA + 1uM PFPA) - Methanol -0.1269909 0.0700558 41 -1.8127103 0.2748554
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.1606428 0.0700558 41 -2.2930695 0.1038691
Table 7 Exposure = 200uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.0289713 0.1085918 41 0.2667913 0.9980908
(1uM PFOA + 1uM PFHpA) - Methanol 0.0244600 0.1085918 41 0.2252472 0.9990164
(1uM PFOA + 1uM PFPA) - Methanol -0.1299630 0.1085918 41 -1.1968037 0.6633084
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.1396104 0.1085918 41 1.2856439 0.6021132
Table 7 Exposure = 400uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.1520717 0.1326753 41 1.1461947 0.6974677
(1uM PFOA + 1uM PFHpA) - Methanol -0.0015508 0.1326753 41 -0.0116887 1.0000000
(1uM PFOA + 1uM PFPA) - Methanol -0.0341789 0.1326753 41 -0.2576129 0.9983348
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.0934303 0.1326753 41 0.7042024 0.9298126

[1] 8

  • Sheet 8: *Please compare each exposure group to the methanol group at each cisplatin dose
    Table 8 Exposure = 0uM Cisplatin
    contrast estimate SE df t.ratio p.value
    (1uM PFHpA + 1uM PFPA) - Methanol -0.0708035 0.0330215 32 -2.1441645 0.1496527
    (1uM PFOA + 1uM PFHpA) - Methanol -0.0370369 0.0330215 32 -1.1215994 0.7166069
    (1uM PFOA + 1uM PFPA) - Methanol -0.0419004 0.0330215 32 -1.2688832 0.6176191
    (750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.0167814 0.0330215 32 -0.5081962 0.9779840
Table 8 Exposure = 2.5uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol -0.1099499 0.0383546 32 -2.8666706 0.0287940
(1uM PFOA + 1uM PFHpA) - Methanol -0.0335124 0.0383546 32 -0.8737518 0.8604156
(1uM PFOA + 1uM PFPA) - Methanol -0.0605810 0.0383546 32 -1.5794986 0.4112877
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.0645390 0.0383546 32 -1.6826935 0.3502004
Table 8 Exposure = 5uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol -0.1672758 0.0447348 32 -3.739278 0.0028934
(1uM PFOA + 1uM PFHpA) - Methanol -0.0580675 0.0447348 32 -1.298040 0.5976265
(1uM PFOA + 1uM PFPA) - Methanol -0.1429386 0.0447348 32 -3.195244 0.0124824
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.1763052 0.0447348 32 -3.941120 0.0016515
Table 8 Exposure = 10uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol -0.2130260 0.0651478 32 -3.269886 0.0102666
(1uM PFOA + 1uM PFHpA) - Methanol -0.0695802 0.0651478 32 -1.068036 0.7508508
(1uM PFOA + 1uM PFPA) - Methanol -0.1549157 0.0651478 32 -2.377910 0.0909338
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol -0.0938966 0.0651478 32 -1.441285 0.5002747
Table 8 Exposure = 25uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol -0.1753631 0.0826781 32 -2.1210346 0.1568695
(1uM PFOA + 1uM PFHpA) - Methanol -0.0635055 0.0826781 32 -0.7681052 0.9071932
(1uM PFOA + 1uM PFPA) - Methanol -0.1804552 0.0826781 32 -2.1826241 0.1382527
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.0712188 0.0826781 32 0.8613983 0.8664041

[1] 9

  • Sheet 9: *Pleasecompare each exposure group to the methanol group at each carboplatin dose
    Table 9 Exposure = 0uM Carboplatin
    contrast estimate SE df t.ratio p.value
    2uM PFHpA - Methanol 0.4128238 0.0794421 47 5.1965375 0.0000302
    2uM PFOA - Methanol 0.1474639 0.0794421 47 1.8562437 0.3969115
    2uM PFPA - Methanol 0.3050904 0.0794421 47 3.8404131 0.0025637
    500nM PFHpA - Methanol 0.2830914 0.0794421 47 3.5634935 0.0059503
    500nM PFOA - Methanol 0.0475019 0.0794421 47 0.5979443 0.9964201
    500nM PFPA - Methanol 0.3535444 0.0794421 47 4.4503413 0.0003671
    Media - Methanol 0.1298940 0.1308816 47 0.9924544 0.9368524
Table 9 Exposure = 50uM Carboplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.3778561 0.0836575 47 4.516704 0.0002953
2uM PFOA - Methanol 0.2189669 0.0836575 47 2.617422 0.0802643
2uM PFPA - Methanol 0.3407626 0.0836575 47 4.073307 0.0012367
500nM PFHpA - Methanol 0.3358016 0.0836575 47 4.014006 0.0014914
500nM PFOA - Methanol 0.1033013 0.0836575 47 1.234813 0.8290779
500nM PFPA - Methanol 0.2276381 0.0836575 47 2.721073 0.0619574
Media - Methanol 0.2463255 0.1378264 47 1.787215 0.4436470
Table 9 Exposure = 100uM Carboplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.3868181 0.0646176 47 5.986267 0.0000020
2uM PFOA - Methanol 0.2321084 0.0646176 47 3.592032 0.0054632
2uM PFPA - Methanol 0.2559041 0.0646176 47 3.960286 0.0017655
500nM PFHpA - Methanol 0.3323580 0.0646176 47 5.143461 0.0000362
500nM PFOA - Methanol 0.1652940 0.0646176 47 2.558035 0.0927584
500nM PFPA - Methanol 0.1531893 0.0646176 47 2.370706 0.1436299
Media - Methanol 0.2279473 0.1064580 47 2.141194 0.2346036
Table 9 Exposure = 200uM Carboplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.3930934 0.0809378 47 4.856732 0.0000954
2uM PFOA - Methanol 0.2185904 0.0809378 47 2.700719 0.0652286
2uM PFPA - Methanol 0.2737219 0.0809378 47 3.381878 0.0101644
500nM PFHpA - Methanol 0.2880968 0.0809378 47 3.559482 0.0060220
500nM PFOA - Methanol 0.1166508 0.0809378 47 1.441240 0.6953027
500nM PFPA - Methanol 0.1385331 0.0809378 47 1.711598 0.4972377
Media - Methanol 0.1709952 0.1333458 47 1.282344 0.8010693
Table 9 Exposure = 400uM Carboplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.4610356 0.0873195 47 5.279869 0.0000227
2uM PFOA - Methanol 0.1695753 0.0873195 47 1.942009 0.3425001
2uM PFPA - Methanol 0.2104549 0.0873195 47 2.410170 0.1313253
500nM PFHpA - Methanol 0.3583884 0.0873195 47 4.104334 0.0011207
500nM PFOA - Methanol 0.1023341 0.0873195 47 1.171949 0.8628915
500nM PFPA - Methanol 0.1846968 0.0873195 47 2.115183 0.2471464
Media - Methanol 0.3090203 0.1438597 47 2.148067 0.2313675

[1] 10

  • Sheet 10: *Please compare each exposure group to the methanol group at each cisplatin dose
    Table 10 Exposure = 0uM Cisplatin
    contrast estimate SE df t.ratio p.value
    2uM PFHpA - Methanol 0.3515951 0.0793683 46 4.4299186 0.0003469
    2uM PFOA - Methanol 0.1192770 0.0793683 46 1.5028296 0.5946352
    2uM PFPA - Methanol 0.0232883 0.0793683 46 0.2934205 0.9998540
    500nM PFHpA - Methanol 0.2681725 0.0793683 46 3.3788360 0.0089133
    500nM PFOA - Methanol 0.0722505 0.0793683 46 0.9103198 0.9359119
    500nM PFPA - Methanol -0.0208950 0.0793683 46 -0.2632668 0.9999225
Table 10 Exposure = 2.5uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.2158975 0.0628361 46 3.4358853 0.0075502
2uM PFOA - Methanol 0.1461892 0.0628361 46 2.3265174 0.1380218
2uM PFPA - Methanol -0.0349594 0.0628361 46 -0.5563592 0.9945627
500nM PFHpA - Methanol 0.2981169 0.0628361 46 4.7443594 0.0001237
500nM PFOA - Methanol 0.0833855 0.0628361 46 1.3270324 0.7197550
500nM PFPA - Methanol -0.0152265 0.0628361 46 -0.2423203 0.9999524
Table 10 Exposure = 5uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.3364875 0.0676293 46 4.9754674 0.0000573
2uM PFOA - Methanol 0.2056453 0.0676293 46 3.0407714 0.0230990
2uM PFPA - Methanol -0.0119654 0.0676293 46 -0.1769265 0.9999926
500nM PFHpA - Methanol 0.3224127 0.0676293 46 4.7673507 0.0001146
500nM PFOA - Methanol 0.1234740 0.0676293 46 1.8257471 0.3710945
500nM PFPA - Methanol -0.0310081 0.0676293 46 -0.4585010 0.9981220
Table 10 Exposure = 10uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.2715554 0.0705676 46 3.8481602 0.0021891
2uM PFOA - Methanol 0.1670716 0.0705676 46 2.3675405 0.1258513
2uM PFPA - Methanol 0.0335971 0.0705676 46 0.4760986 0.9976839
500nM PFHpA - Methanol 0.3585750 0.0705676 46 5.0812984 0.0000401
500nM PFOA - Methanol 0.1863466 0.0705676 46 2.6406823 0.0657048
500nM PFPA - Methanol 0.0394981 0.0705676 46 0.5597203 0.9943832
Table 10 Exposure = 25uM Cisplatin
contrast estimate SE df t.ratio p.value
2uM PFHpA - Methanol 0.3849417 0.0738754 46 5.210687 0.0000259
2uM PFOA - Methanol 0.2579887 0.0738754 46 3.492214 0.0064003
2uM PFPA - Methanol 0.0781715 0.0738754 46 1.058153 0.8777508
500nM PFHpA - Methanol 0.4626299 0.0738754 46 6.262299 0.0000007
500nM PFOA - Methanol 0.1897629 0.0738754 46 2.568687 0.0784203
500nM PFPA - Methanol 0.0452086 0.0738754 46 0.611957 0.9909595

[1] 11

  • Sheet 11: *Pleasecompare each exposure group to the methanol group at each carboplatin dose
    Table 11 Exposure = 0uM Carboplatin
    contrast estimate SE df t.ratio p.value
    (1uM PFHpA + 1uM PFPA) - Methanol 0.3140013 0.0719125 32 4.3664366 0.0004953
    (1uM PFOA + 1uM PFHpA) - Methanol 0.0306617 0.0719125 32 0.4263759 0.9885226
    (1uM PFOA + 1uM PFPA) - Methanol 0.1803882 0.0719125 32 2.5084407 0.0677623
    (750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.4535254 0.0719125 32 6.3066305 0.0000018
Table 11 Exposure = 50uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.2768588 0.0746126 32 3.710616 0.0031311
(1uM PFOA + 1uM PFHpA) - Methanol 0.0780964 0.0746126 32 1.046691 0.7641036
(1uM PFOA + 1uM PFPA) - Methanol 0.2137480 0.0746126 32 2.864771 0.0289297
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.3463117 0.0746126 32 4.641463 0.0002246
Table 11 Exposure = 100uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.2886991 0.0710674 32 4.062327 0.0011750
(1uM PFOA + 1uM PFHpA) - Methanol 0.1371292 0.0710674 32 1.929565 0.2277456
(1uM PFOA + 1uM PFPA) - Methanol 0.2343871 0.0710674 32 3.298095 0.0095311
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.3977075 0.0710674 32 5.596199 0.0000140
Table 11 Exposure = 200uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.3362988 0.0579478 32 5.803478 0.0000077
(1uM PFOA + 1uM PFHpA) - Methanol 0.0599172 0.0579478 32 1.033986 0.7718721
(1uM PFOA + 1uM PFPA) - Methanol 0.2138907 0.0579478 32 3.691093 0.0033037
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.3671876 0.0579478 32 6.336523 0.0000016
Table 11 Exposure = 400uM Carboplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.3800020 0.0653361 32 5.816112 0.0000074
(1uM PFOA + 1uM PFHpA) - Methanol 0.1148241 0.0653361 32 1.757438 0.3094382
(1uM PFOA + 1uM PFPA) - Methanol 0.2294164 0.0653361 32 3.511327 0.0053918
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.4227050 0.0653361 32 6.469702 0.0000011

[1] 12

  • Sheet 12: *Please compare each exposure group to the methanol group at each cisplatin dose
    Table 12 Exposure = 0uM Cisplatin
    contrast estimate SE df t.ratio p.value
    (1uM PFHpA + 1uM PFPA) - Methanol 0.1119336 0.0686556 32 1.6303632 0.3805200
    (1uM PFOA + 1uM PFHpA) - Methanol -0.0705542 0.0686556 32 -1.0276529 0.7757093
    (1uM PFOA + 1uM PFPA) - Methanol 0.0295624 0.0686556 32 0.4305894 0.9880909
    (750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.2060543 0.0686556 32 3.0012730 0.0205511
Table 12 Exposure = 2.5uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.1615684 0.0816781 32 1.9781124 0.2078268
(1uM PFOA + 1uM PFHpA) - Methanol 0.0373682 0.0816781 32 0.4575058 0.9850620
(1uM PFOA + 1uM PFPA) - Methanol 0.0898981 0.0816781 32 1.1006394 0.7301615
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.2326222 0.0816781 32 2.8480376 0.0301515
Table 12 Exposure = 5uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.2060090 0.0837157 32 2.460816 0.0755304
(1uM PFOA + 1uM PFHpA) - Methanol 0.0888579 0.0837157 32 1.061424 0.7549824
(1uM PFOA + 1uM PFPA) - Methanol 0.1119500 0.0837157 32 1.337263 0.5707334
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.2486246 0.0837157 32 2.969867 0.0222486
Table 12 Exposure = 10uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.1899030 0.0776021 32 2.4471383 0.0779020
(1uM PFOA + 1uM PFHpA) - Methanol 0.0343918 0.0776021 32 0.4431813 0.9867333
(1uM PFOA + 1uM PFPA) - Methanol 0.1254189 0.0776021 32 1.6161798 0.3889758
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.1999801 0.0776021 32 2.5769949 0.0578270
Table 12 Exposure = 25uM Cisplatin
contrast estimate SE df t.ratio p.value
(1uM PFHpA + 1uM PFPA) - Methanol 0.2915678 0.0764222 32 3.8152239 0.0023453
(1uM PFOA + 1uM PFHpA) - Methanol 0.0285555 0.0764222 32 0.3736538 0.9930364
(1uM PFOA + 1uM PFPA) - Methanol 0.1471300 0.0764222 32 1.9252262 0.2295923
(750nM PFOA + 750nM PFHpA + 750nM PFPA) - Methanol 0.2847267 0.0764222 32 3.7257057 0.0030037