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Study Information
Study Attribute Value
CEBS Accession Number: 002-02888-0002-0000-0
Chemical Name: Tetrakis(hydroxymethyl)phosphonium chloride
CASRN: 124-64-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 523400_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  Positive 
No Activation  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 01/07/1986    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
200 4 0.020 2.0 0 0.000 0.0 3 0.015 1.5 1 0.005 1.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride
9.99      
200 5 0.025 2.0 0 0.000 0.0 5 0.025 2.0 0 0.000 0.0
30         
50 25 0.500 32.0 12 0.240 22.0 13 0.260 18.0 0 0.000 0.0
40         
100 44 0.440 25.0 19 0.190 15.0 15 0.150 13.0 10 0.100 1.0
50         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.2       
50 136 2.720 78.0 23 0.460 32.0 43 0.860 52.0 70 1.400 7.0
Mitomycin-C
1         
15 150 10.000 100.0 0 0.000 0.0 0 0.000 0.0 150 10.000 15.0
Trend: 8.644 7.881 5.652  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 01/31/1985    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride
10         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
30         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
50         
100 6 0.060 6.0 2 0.020 2.0 4 0.040 4.0 0 0.000 0.0
150         
15 5 0.333 27.0 2 0.133 13.0 3 0.200 20.0 0 0.000 0.0
Positive Control: Cyclophosphamide
50         
50 56 1.120 44.0 22 0.440 30.0 23 0.460 32.0 11 0.220 2.0
Trend: 3.748 3.093 2.922  
Probability: 0.000 0.001 0.002  


Trial #:2   Activation: No Activation    Date: 02/22/1985    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride
15         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
30         
100 7 0.070 6.0 0 0.000 0.0 7 0.070 6.0 0 0.000 0.0
45         
99 20 0.202 19.0 3 0.030 3.0 17 0.172 16.0 0 0.000 0.0
Positive Control: Mitomycin-C
5         
50 48 0.960 56.0 15 0.300 26.0 33 0.660 40.0 0 0.000 0.0
Trend: 5.231 1.625 4.943  
Probability: 0.000 0.052 0.000  


Trial #:3   Activation: No Activation    Date: 01/31/1985    Harvest Time: 10.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride
10         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
30         
100 10 0.100 10.0 1 0.010 1.0 9 0.090 9.0 0 0.000 0.0
50         
90 4 0.044 4.0 0 0.000 0.0 4 0.044 4.0 0 0.000 0.0
Positive Control: Mitomycin-C
5         
50 20 0.400 28.0 7 0.140 12.0 13 0.260 20.0 0 0.000 0.0
Trend: 2.297 0.698 2.186  
Probability: 0.011 0.243 0.014