COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02888-0002-0000-0
Chemical Name: Tetrakis(hydroxymethyl)phosphonium chloride
CASRN: 124-64-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 523400_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Positive 
No Activation  3  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 01/07/1986    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 0 0.000 0.0 3 0.015 1.5 1 0.005 1.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride 
9.99      
 200 5 0.025 2.0 0 0.000 0.0 5 0.025 2.0 0 0.000 0.0 
30         
 50 25 0.500 32.0 12 0.240 22.0 13 0.260 18.0 0 0.000 0.0 
40         
 100 44 0.440 25.0 19 0.190 15.0 15 0.150 13.0 10 0.100 1.0 
50         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 50 136 2.720 78.0 23 0.460 32.0 43 0.860 52.0 70 1.400 7.0
Mitomycin-C 
1         
 15 150 10.000 100.0 0 0.000 0.0 0 0.000 0.0 150 10.000 15.0
Trend: 8.644 7.881 5.652  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 01/31/1985    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride 
10         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
30         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
50         
 100 6 0.060 6.0 2 0.020 2.0 4 0.040 4.0 0 0.000 0.0 
150         
 15 5 0.333 27.0 2 0.133 13.0 3 0.200 20.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 56 1.120 44.0 22 0.440 30.0 23 0.460 32.0 11 0.220 2.0
Trend: 3.748 3.093 2.922  
Probability: 0.000 0.001 0.002  


Trial #:2   Activation: No Activation    Date: 02/22/1985    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride 
15         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
30         
 100 7 0.070 6.0 0 0.000 0.0 7 0.070 6.0 0 0.000 0.0 
45         
 99 20 0.202 19.0 3 0.030 3.0 17 0.172 16.0 0 0.000 0.0
Positive Control: Mitomycin-C 
5         
 50 48 0.960 56.0 15 0.300 26.0 33 0.660 40.0 0 0.000 0.0
Trend: 5.231 1.625 4.943  
Probability: 0.000 0.052 0.000  


Trial #:3   Activation: No Activation    Date: 01/31/1985    Harvest Time: 10.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetrakis(hydroxymethyl)phosphonium chloride 
10         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
30         
 100 10 0.100 10.0 1 0.010 1.0 9 0.090 9.0 0 0.000 0.0 
50         
 90 4 0.044 4.0 0 0.000 0.0 4 0.044 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
5         
 50 20 0.400 28.0 7 0.140 12.0 13 0.260 20.0 0 0.000 0.0
Trend: 2.297 0.698 2.186  
Probability: 0.011 0.243 0.014