RR-12: NTP Research Report on Synthetic Turf/Recycled Tire Crumb Rubber: Characterization of the Leachability and Cytotoxicity of Crumb Rubber In Vitro

Study Data

Study Data

• CellTiter-Glo (CTG) viability/cytotoxicity assay (16 KB)

Human Lung Cells

24 Hour Exposure

• 24 hr exposure of human lung cells to crumb rubber-conditioned media (37°C) was cytotoxic (46 KB)
• 24 hr exposure of human lung cells to crumb rubber-conditioned media (60°C) was cytotoxic (54 KB)
• 24 hr exposure of human lung cells to crumb rubber-conditioned media (RT) was cytotoxic (46 KB)
• 24 hr exposure of human lung cells to crumb rubber-conditioned PBS or ALF (60°C) was not cytotoxic (16 KB)

72 Hour Exposure

• 72 hr exposure of human lung cells to crumb rubber-conditioned media (37°C) was cytotoxic (40 KB)
• 72 hr exposure of human lung cells to crumb rubber-conditioned media (60°C) was cytotoxic (40 KB)
• 72 hr exposure of human lung cells to crumb rubber-conditioned media (RT) was cytotoxic (40 KB)

Human Skin Cells

24 Hour Exposure

• 24 hr exposure of human skin cells to crumb rubber-conditioned media (37°C) was cytotoxic (46 KB)
• 24 hr exposure of human skin cells to crumb rubber-conditioned media (60°C) was cytotoxic (46 KB)
• 24 hr exposure of human skin cells to crumb rubber-conditioned media (RT) was cytotoxic (46 KB)

72 Hour Exposure

• 72 hr exposure of human skin cells to crumb rubber-conditioned media (37°C) was cytotoxic (40 KB)
• 72 hr exposure of human skin cells to crumb rubber-conditioned media (60°C) was cytotoxic (46 KB)
• 72 hr exposure of human skin cells to crumb rubber-conditioned media (RT) was cytotoxic (39 KB)

Human Small Intestine Cells

24 Hour Exposure

• 24 hr exposure of human small intestinal cells but not HepaRG cells to crumb rubber-conditioned media (60°C) was cytotoxic (14 KB)