Screen for Developmental Toxicity in Zebrafish- Study 1
Celia Quevedo, Mamta Behl, Kristen Ryan, Richard S. Paules, Aintzane Alday, Arantza Muriana, and Ainhoa Alzualde. 2019. Detection and Prioritization of Developmentally Neurotoxic and/or Neurotoxic Compounds Using Zebrafish. TOXICOLOGICAL SCIENCES, 168(1), 2019, 225–240. doi: 10.1093/toxsci/kfy291
DOI:
https://doi.org/10.22427/NTP-DATA-002-00077-0001-0000-7
Materials and Methods
Study Description
A full description of the materials and methods can be found in Quevedo et al., 2019. All experiments were conducted at Biobide (Donostia-San Sebastián, Spain). Eighty-seven chemicals tested were provided by the National Toxicology Program (Research Triangle Park, NC, USA) and are cited in Quevdo et al., 2019. An additional chemical, 2-((1-(4-Phenoxyphenoxy)propan-2-yl)oxy)pyridine: MPEP (CASRN: 95737-68-1) was evaluated in parallel and thus there are results for a total of 88 chemicals. In brief, the adult zebrafish line Tg(Cmlc2: copGFP) was used to evaluate embryo toxicity following chemical exposure. Eighty-eight compounds were evaluated in dose response with ~8 concentrations of each chemical. Fertilized embryos, at 4 hours post fertilization, were placed in 24-well plates with the corresponding chemical concentration or 0.5% DMSO as a negative control. The plates were covered and wrapped with aluminum foil to avoid degradation of light-sensitive compounds. Fifteen embryos were analyzed per condition after 2 and 4 days of incubation (i.e. 2 and 4 days post fertilization (dpf)) at 28.5°C. Detailed analysis of embryo morphology (including malformations in the head, heart and tail, deformed body shape, and the presence of edemas) and lethality was performed at 2 and 4 dpf. Embryo morphology was visualized under a stereo microscope (Olympus SXZ10) by experienced technicians. Morphologies found on study are recorded as present (1) or absent (0).
Data
Results
- Zebrafish 1-Ethyl-3-methylimidazolium diethylphosphate CASRN 848641-69-0 (20 KB)
- Zebrafish 1-Methyl-4-phenylpyridinium iodide CASRN 36913-39-0 (20 KB)
- Zebrafish 2,2',4,4',5,5'-Hexabromodiphenyl ether (BDE-153) CASRN 68631-49-2 (20 KB)
- Zebrafish 2,2',4,4',5-Pentabromodiphenyl ether (BDE-99) CASRN 60348-60-9 (21 KB)
- Zebrafish 2,2',4,4'-Tetrabromodiphenyl ether CASRN 5436-43-1 (27 KB)
- Zebrafish 2,3,7,8-Tetrachlorodibenzo-p-dioxin CASRN 1746-01-6 (27 KB)
- Zebrafish 2-Ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB) CASRN 183658-27-7 (20 KB)
- Zebrafish 2-Ethylhexyl diphenyl phosphate (EHDP) CASRN 1241-94-7 (28 KB)
- Zebrafish 2-Methoxyethanol CASRN 109-86-4 (22 KB)
- Zebrafish 3,3'-Iminodipropionitrile CASRN 111-94-4 (21 KB)
- Zebrafish 3,3’,5,5’-Tetrabromobisphenol A CASRN 79-94-7 (27 KB)
- Zebrafish 4-H-Cyclopenta(d,e,f)phenanthrene CASRN 203-64-5 (27 KB)
- Zebrafish 5-Fluorouracil CASRN 51-21-8 (20 KB)
- Zebrafish 5-nitro-2-furaldehyde semicarbazone CASRN 59-87-0 (23 KB)
- Zebrafish 6-Hydroxydopamine hydrochloride CASRN 28094-15-7 (20 KB)
- Zebrafish 6-Propyl-2-thiouracil CASRN 51-52-5 (20 KB)
- Zebrafish Acenaphthene CASRN 83-32-9 (27 KB)
- Zebrafish Acenaphthylene CASRN 208-96-8 (28 KB)
- Zebrafish Acetaminophen (4-hydroxyacetanilide) CASRN 103-90-2 (21 KB)
- Zebrafish Acetic acid, manganese (2+) salt CASRN 638-38-0 (21 KB)
- Zebrafish Acetylsalicylic acid CASRN 50-78-2 (20 KB)
- Zebrafish Acrylamide CASRN 79-06-1 (21 KB)
- Zebrafish Aldicarb CASRN 116-06-3 (27 KB)
- Zebrafish Amoxicillin CASRN 26787-78-0 (20 KB)
- Zebrafish Anthracene CASRN 120-12-7 (21 KB)
- Zebrafish Auramine O CASRN 2465-27-2 (27 KB)
- Zebrafish Benz(a)anthracene CASRN 56-55-3 (27 KB)
- Zebrafish Benzo(a)pyrene CASRN 50-32-8 (26 KB)
- Zebrafish Benzo(b)fluoranthene CASRN 205-99-2 (27 KB)
- Zebrafish Benzo(e)pyrene CASRN 192-97-2 (27 KB)
- Zebrafish Benzo(k)fluoranthene CASRN 207-08-9 (27 KB)
- Zebrafish Benzog,h,i perylene CASRN 191-24-2 (20 KB)
- Zebrafish Berberine chloride CASRN 633-65-8 (20 KB)
- Zebrafish Bis(2-ethylhexyl) 3,4,5,6- tetrabromophthalate (TBPH) CASRN 26040-51-7 (20 KB)
- Zebrafish Bis(tributyltin)oxide CASRN 56-35-9 (27 KB)
- Zebrafish Bisphenol A CASRN 80-05-7 (27 KB)
- Zebrafish Bisphenol AF CASRN 1478-61-1 (27 KB)
- Zebrafish Bisphenol S CASRN 80-09-1 (20 KB)
- Zebrafish Captan CASRN 133-06-2 (27 KB)
- Zebrafish Carbamic acid, butyl-, 3-iodo-2-propynyl ester CASRN 55406-53-6 (27 KB)
- Zebrafish Carbaryl CASRN 63-25-2 (27 KB)
- Zebrafish Chlorpyrifos (Dursban) CASRN 2921-88-2 (27 KB)
- Zebrafish Chrysene CASRN 218-01-9 (20 KB)
- Zebrafish Colchicine CASRN 64-86-8 (26 KB)
- Zebrafish D-Glucitol CASRN 50-70-4 (20 KB)
- Zebrafish Deltamethrin CASRN 52918-63-5 (44 KB)
- Zebrafish Di(2-ethylhexyl) phthalate CASRN 117-81-7 (21 KB)
- Zebrafish Diazepam CASRN 439-14-5 (27 KB)
- Zebrafish Dibenz(a,h)anthracene CASRN 53-70-3 (27 KB)
- Zebrafish Dibenz a,c anthracene CASRN 215-58-7 (20 KB)
- Zebrafish Dichlorodiphenyltrichloroethane (DDT) CASRN 50-29-3 (27 KB)
- Zebrafish Dieldrin CASRN 60-57-1 (27 KB)
- Zebrafish Diethylstilbestrol CASRN 56-53-1 (27 KB)
- Zebrafish Estradiol CASRN 50-28-2 (27 KB)
- Zebrafish Firemaster 550 CASRN 860302-33-6 (28 KB)
- Zebrafish Fluorene CASRN 86-73-7 (27 KB)
- Zebrafish Heptachlor CASRN 76-44-8 (27 KB)
- Zebrafish Hexachlorophene CASRN 70-30-4 (27 KB)
- Zebrafish Hydroxyurea CASRN 127-07-1 (20 KB)
- Zebrafish Isodecyl diphenyl phosphate CASRN 29761-21-5 (26 KB)
- Zebrafish L-Ascorbic acid CASRN 50-81-7 (20 KB)
- Zebrafish Lead (II) acetate trihydrate CASRN 6080-56-4 (26 KB)
- Zebrafish Lindane CASRN 58-89-9 (27 KB)
- Zebrafish Manganese, tricarbonyl (1,2,3,4,5-.eta.)-1-methyl-2,4-cyclopentadien-1-yl- CASRN 12108-13-3 (21 KB)
- Zebrafish Methyl mercuric (II) chloride CASRN 115-09-3 (45 KB)
- Zebrafish MPEP CASRN 95737-68-1 (28 KB)
- Zebrafish n-Hexane CASRN 110-54-3 (26 KB)
- Zebrafish Naphthalene CASRN 91-20-3 (26 KB)
- Zebrafish Parathion CASRN 56-38-2 (27 KB)
- Zebrafish Permethrin CASRN 52645-53-1 (27 KB)
- Zebrafish Phenanthrene CASRN 85-01-8 (27 KB)
- Zebrafish Phenobarbital CASRN 50-06-6 (20 KB)
- Zebrafish Phenobarbital sodium salt CASRN 57-30-7 (27 KB)
- Zebrafish Phenol, isopropylated, phosphate (3 to1) CASRN 68937-41-7 (28 KB)
- Zebrafish Pyrene CASRN 129-00-0 (27 KB)
- Zebrafish Rotenone CASRN 83-79-4 (28 KB)
- Zebrafish Saccharin Sodium Salt hydrate CASRN 82385-42-0 (32 KB)
- Zebrafish Tebuconazole CASRN 107534-96-3 (27 KB)
- Zebrafish tert-Butylphenyl diphenyl phosphate CASRN 56803-37-3 (28 KB)
- Zebrafish Tetraethylthiuram disulfide CASRN 97-77-8 (27 KB)
- Zebrafish Thalidomide CASRN 50-35-1 (26 KB)
- Zebrafish Toluene CASRN 108-88-3 (20 KB)
- Zebrafish Tricresyl phosphate CASRN 1330-78-5 (27 KB)
- Zebrafish Triphenyl phosphate CASRN 115-86-6 (45 KB)
- Zebrafish Tris(2-Chloroethyl) Phosphate CASRN 115-96-8 (20 KB)
- Zebrafish tris(Chloropropyl) phosphate, TCPP CASRN 13674-84-5 (26 KB)
- Zebrafish Valinomycin CASRN 2001-95-8 (28 KB)
- Zebrafish Valproic acid sodium salt CASRN 1069-66-5 (26 KB)