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Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT): Interlaboratory Study Dose Range Finding (DRF) data

DOI: https://doi.org/10.22427/NTP-DATA-002-00102-0001-000-6

General Information

The small size and rapid development of the zebrafish make it a useful vertebrate model for assessing the potential effects of substances on growth and development using high-throughput screening methods. The embryonic zebrafish model has been used for this purpose in pharmaceutical development and in high-throughput screening programs at NTP and the EPA. However, broader adoption of the zebrafish model for toxicological screening is hindered by deficits in:

• Consistent experimental protocol elements.
• Clear understanding of mechanisms of chemical absorption, distribution, metabolism, and excretion in zebrafish.
• Consistent informatics approaches used for classification of outcomes.

To enable the broader adoption of zebrafish for toxicological screening, the Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT) program was developed by the NIEHS Division of Translational Toxicology (formerly Division of the National Toxicology Program) and NICEATM scientists. As a result of several information gathering activities (SEAZIT), an Interlaboratory Study was designed to determine the effect of chorion removal and exposure media renewal on chemical potency by quantifying effects on mortality or abnormal phenotypes (i.e., developmental toxicity). Participating laboratories used in-house protocols to test a defined chemical set while varying the two protocol elements under investigation. The chemical set, which was designed to provide overlap with other studies from our organization, included chemicals with a range of physicochemical properties and literature suggesting their ability to cause developmental toxicity. Many of the chemicals have in vivo reference data available from rodent and other zebrafish studies. The Interlaboratory Study included was conducted in 2 phases: The dose range-finding (DRF) phase and the Definitive phase.

Data Release Information

The data release of the SEAZIT DRF data. The DRF data contains three datasets: 1) Dose Range Finding study, Lab A, Static Renewal-Chorion (SR-C) 2) Dose Range Finding study, Lab B, Static-Dechorion (S-DC) 3) Dose Range Finding study, Lab C, Static-Chorion (S-C). The three datasets were collected from three individual laboratories (Lab A, B, C), each of which used one of the three test conditions (SR-C, S-DC, S-C). The files in the data release include a high-level result summary file (specificity.txt), metadata files (ontology.txt and substance.txt), intermediate-level data process files (bmc_input.txt, bmc_output.txt), and a low-level data file (plate.txt).

Related Publications

Related Publications

Jui-Hua Hsieh, Sue Nolte, Jon T Hamm, Zicong Wang, Georgia K Roberts, Charles P Schmitt, Kristen R Ryan Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT): Developing a Data Analysis Pipeline for the Assessment of Developmental Toxicity with an Interlaboratory Study